Rotation frequency of human bronchial and nasal epithelial spheroids as an indicator of mucociliary function

Respiration

Volumn 73, Issue 5, 2006, Pages 664-672

  Gamarra, Fernando ^a   Bergner, Albrecht ^a   Stauss, Elisabeth ^a   Stocker, Ingrid ^a   Grundler, Stefan ^a   Huber, Rudolf Maria ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

Functional assay; Isoproterenol; Mucociliary function; Mucus transport velocity; Rotation; Spheroids

Indexed keywords

ISOPRENALINE;

ADULT; AGED; ARTICLE; BRONCHUS MUCOSA; CELL SURFACE; CILIARY MOTILITY; CONCENTRATION (PARAMETERS); HUMAN; HUMAN CELL; ILLUMINATION; IN VITRO STUDY; NOSE MUCOSA; PHOTOLYSIS; PRIORITY JOURNAL; ROTATION; SPHEROID CELL; TEMPERATURE; VELOCITY;

BRONCHODILATOR AGENTS; HUMANS; ISOPROTERENOL; MUCOCILIARY CLEARANCE; NASAL MUCOSA; RESPIRATORY MUCOSA; ROTATION; SPHEROIDS, CELLULAR; TEMPERATURE; TIME FACTORS;

EID: 33749253321     PISSN: 00257931     EISSN: None     Source Type: Journal    
DOI: 10.1159/000092672     Document Type: Article

References (21)

1. Jorissen M: Correlations among mucociliary transport, ciliary function, and ciliary structure. Am J Rhinol 1998;12:53-58.
2. Corssen G, Allen CR: Acetylcholine: its significance in controlling ciliary activity of human respiratory epithelium in vitro. J Appl Physiol 1959;14:901-904.
3. Jorissen M, Van der SB, Tyberghein J, Van der BH, Cassiman JJ: Ciliogenesis and coordinated ciliary beating in human nasal epithelial cells cultured in vitro. Acta Otorhinolaryngol Belg 1989;43:67-73.
4. Sanderson MJ, Dirksen ER: Mechanosensitivity of cultured ciliated cells from the mammalian respiratory tract: implications for the regulation of mucociliary transport. Proc Natl Acad Sci USA 1986;83:7302-7306.
5. Al Batran SE, Astner ST, Supthut M, Gamarra F, Brueckner K, Welsch U, Knuechel R, Huber RM: Three-dimensional in vitro cocultivation of lung carcinoma cells with human bronchial organ culture as a model for bronchial carcinoma. Am J Respir Cell Mol Biol 1999;21:200-208.
6. Gamarra F, Wagner S, Al Batran S, Maier I, Castro M, Hautmann H, Bergner A, Baumgartner R, Huber RM: Kinetics of 5-aminolevulinic acid-induced fluorescence in organ cultures of bronchial epithelium and tumor. Respiration 2002;69:445-450.
7. Adams SR, Tsien RY: Controlling cell chemistry with caged compounds. Annu Rev Physiol 1993;55:755-784.
8. Bridges MA, Walker DC, Harris RA, Wilson BR, Davidson AG: Cultured human nasal epithelial multicellular spheroids: polar cyst-like model tissues. Biochem Cell Biol 1991;69:102-108.
9. Armengot M, Basterra J, Garcia-Bartual E: The influence of anesthetics and vasoconstrictors on nasal mucociliary transport. Acta Otorhinolaryngol Belg 1989;43:149-156.
10. Morgan L, Pearson M, de Iongh R, Mackey D, van der Wall H, Peters M, Rutland J: Scintigraphic measurement of tracheal mucus velocity in vivo. Eur Respir J 2004;23:518-522.
11. Wanner A, Salathe M, O'Riordan TG: Mucociliary clearance in the airways. Am J Respir Crit Care Med 1996;154:1868-1902.
12. Jorissen M, Bessems A: Influence of culture duration and ciliogenesis on the relationship between ciliary beat frequency and temperature in nasal epithelial cells. Eur Arch Otorhinolaryngol 1995;252:451-454.
13. Kurosawa H, Wang CG, Dandurand RJ, King M, Eidelman DH: Mucociliary function in the mouse measured in explanted lung tissue. J Appl Physiol 1995;79:41-46.
14. Mercke U, Hakansson CH, Toremalm NG: The influence of temperature on mucociliary activity. Temperature range 20 degrees C-40 degrees C. Acta Otolaryngol 1974;78:444-450.
15. Mercke U: The influence of temperature on mucociliary activity. Temperature range 40 degrees C to 50 degrees C. Acta Otolaryngol 1974;78:253-258.
16. Kilgour E, Rankin N, Ryan S, Pack R: Mucociliary function deteriorates in the clinical range of inspired air temperature and humidity. Intensive Care Med 2004;30:1491-1494.
17. Agu RU, Jorissen M, Willems T, Van den Mooter G, Kinget R, Augustijns P: Effects of pharmaceutical compounds on ciliary beating in human nasal epithelial cells: a comparative study of cell culture models. Pharm Res 1999;16:1380-1385.
18. Wyatt TA, Spurzem JR, May K, Sisson JH: Regulation of ciliary beat frequency by both PKA and PKG in bovine airway epithelial cells. Am J Physiol 1998;275:L827-L835.
19. Sanderson MJ, Dirksen ER: Mechanosensitive and beta-adrenergic control of the ciliary beat frequency of mammalian respiratory tract cells in culture. Am Rev Respir Dis 1989;139:432-440.
20. Muralidharan S, Nerbonne JM: Photolabile 'caged' adrenergic receptor agonists and related model compounds. J Photochem Photobiol B 1995;27:123-137.
21. Muralidharan S, Maher GM, Boyle WA, Nerbonne JM: 'Caged' phenylephrine: development and application to probe the mechanism of alpha-receptor-mediated vasoconstriction. Proc Natl Acad Sci USA 1993;90:5199-5203.