Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH

Infection and Immunity

Volumn 74, Issue 10, 2006, Pages 6001-6005

  Zhong, Yingbin ^a   Zhang, Xiao Hua ^a   Chen, Jixiang ^a   Chi, Zhenghao ^a   Sun, Boguang ^a   Li, Yun ^a   Austin, Brian ^b  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b HERIOT WATT UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

HEMOLYSIN; HEMOLYSIN VHH; PHOSPHOLIPASE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; CYTOTOXICITY; ENZYME ACTIVITY; ESCHERICHIA COLI; FISH; FLOUNDER; GENE OVEREXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; TEMPERATURE DEPENDENCE; VHHA GENE; VIBRIO HARVEYI;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; ESCHERICHIA COLI; FLOUNDER; HEMOLYSIN PROTEINS; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASES; TEMPERATURE; VIBRIO;

EID: 33749242440     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.00512-06     Document Type: Article

References (35)

1. Alvarez, J. D., B. Austin, A. M. Alvarez, and H. Reyes. 1998. Vibrio harveyi: a pathogen of penaeid shrimps and fish in Venezuela. J. Fish Dis. 21:313-316.
2. Austin, B., and D. A. Austin. 1989. Methods for the microbiological examination of fish and shellfish. Ellis Horwood, Chichester, United Kingdom.
3. Austin, B. A., C. Pride, and G. A. Rhodie. 2003. Association of a bacteriophage with virulence in Vibrio harveyi. J. Fish Dis. 26:55-58.
4. Crowe, J., B. S. Masone, and J. Ribbe. 1996. One-step purification of recombinant proteins with the 6×His tag and Ni-NTA resin. Methods Mol. Biol. 58:491-510.
5. Han, J.-H., J.-H. Lee, Y.-H. Choi, J.-H. Park, T.-J. Choi, and I.-S. Kong. 2002. Purification, characterization and molecular cloning of'Vibrio fluvialis hemolysin. Biochim. Biophys. Acta 1599:106-114.
6. Hispano, C., Y. Nebra, and A. R. Blanch. 1997. Isolation of Vibrio harveyi from an ocular lesion in the short sunfish (Mola mola). Bull. Eur. Assoc. Fish Pathol. 17:104-107.
7. Ishimaru, K., and K. Muroga. 1997. Taxonomical re-examination of two pathogenic Vibrio species isolated from milkfish and swimming crab. Fish Pathol. 32:59-64.
8. Ju, S.-A., and J.-W. Ju. 1996. Purification and immunological characteristical characterization of β-hemolysin produced by Vibrio mimicus. J. Korean Soc. Microbiol. 31:433-449.
9. Kothary, M. H., H. Lowman, B. A. McCardell, and B. D. Tall. 2003. Purification and characterization of enterotoxigenic E1 Tor-like hemolysin produced by Vibrio fluvialis. Infect. Immun. 71:3213-3220.
10. Kraxberger-Beatty, T., D. J. McGarey, H. J. Grier, and D. V. Lim. 1990. Vibrio harveyi, an opportunistic pathogen of common snook, Centropomus undecimalis (Bloch), held in captivity. J. Fish Dis. 13:557-560.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
12. Lee, J.-H., S.-H. Ahn, S.-H. Kim, Y.-H. Choi, K.-J. Park, and I.-S. Kong. 2002. Characterization of Vibrio mimicus phospholipase A (PhlA) and cytotoxicity on fish cell. Biochem. Biophys. Res. Commun. 298:269-276.
13. Lee, K. K., P. C. Liu, and S. N. Chen. 1996. Studies on the virulence factors of Vibrio for tiger prawn, Penaeus monodon and immunization trials. Rep. Fish Dis. Res. 17:1-13.
14. Lee, K. K., P. C. Liu, G. H. Kou, and S. N. Chen. 1997. Investigation on the major exotoxin of Vibrio harveyi 770527 isolated from diseased Penaeus monodon. Rep. Fish Dis. Res. 18:33-42.
15. Liu, P. C., K. K. Lee, K. C. Yii, G. H. Kou, and S. N. Chen. 1996. Isolation of Vibrio harveyi from diseased Kuruma prawns Penaeus japonicus. Curr. Microbiol. 33:129-132.
16. Liu, P. C., K. K. Lee, and S. N. Chen. 1996. Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon. Lett. Appl. Microbiol. 22:413-416.
17. Liu, P. C., K. K. Lee, C. C. Tu, and S. N. Chen. 1997. Purification and characterization of a cysteine protease produced by pathogenic luminous Vibrio harveyi. Curr. Microbiol. 35:32-39.
18. McCardell, B. A., A. H. Kothary, and J. M. Madden. 1999. Two-step purification and partial characterization of a variant of the Vibrio cholerae non-O1 hemolysin. FEMS Microbiol. Lett. 180:177-182.
19. Mergulhão, F. J. M., D. K. Summers, and G. A. Monteiro. 2005. Recombinant protein secretion in Escherichia coli. Biotechnol. Adv. 23:177-202.
20. Montero, A. B., and B. Austin. 1999. Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post-larval Penaeus vannamei (Bonne). J. Fish Dis. 22:377-386.
21. Niu, X., and M. J. Guiltinan. 1994. DNA binding specificity of the wheat bZIP protein EmBP21. Nucleic Acids Res. 22:4969-4978.
22. Pal, S., B. Guhathakurta, D. Sasmal, R. Mallick, and A. Datta. 1997. Purification and characterization of a hemolysin with phospholipase C activity from Vibrio cholerae O139. FEMS Microbiol. Lett. 147:115-120.
23. Ramesh, A., and V. K. Venugopalan. 1989. Response of enteric luminous bacteria to environmental conditions in the gut of the fish. J. Appl. Bacteriol. 66:529-533.
24. Ruby, E. G., and J. G. Morin. 1979. Luminous enteric bacteria of the marine fishes: a study of their distribution, densities, and dispersion. Appl. Environ. Microbiol. 38:406-411.
25. Shinoda, S., H. Matsuoka, T. Tsuchie, S.-I. Miyoshi, S. Yamamoto, H. Taniguchi, and Y. Mizuguchi. 1991. Purification and characterization of a lecithin-dependent hemolysin from Escherichia coli transformed by a Vibrio parahaemolyticus gene. J. Gen. Microbiol. 137:2705-2711.
26. Taniguchi, H., H. Hirano, S. Kubomura, K. Higashi, and Y. Mizuguchi. 1986. Comparison of the nucleotide sequences of the genes for the thermostable direct hemolysin and the thermolabile hemolysin from Vibrio parahaemolyticus. Microb. Pathog. 1:425-432.
27. Testa, J., L. W. Daniel, and A. S. Kreger. 1984. Extracellular phospholipase A2 and lysophospholipase produced by Vibrio vulnificus. Infect. Immun. 45:458-463.
28. Tong, S. L., H. Li, and H. Z. Miao. 1997. The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus. Aquaculture 156:327-333.
29. Tsunasawa, S., A. Suginara, T. Masaki, F. Sakiyama, Y. Takeda, T. Miwatani, and K. Narita. 1987. Amino acid sequence of thermostable direct hemolysin produced by Vibrio parahaemolyticus. J. Biochem. 101:111-121.
30. Vandenberghe, J., Y. Li, L. Verdonk, J. Li, P. Sorgeloos, H. S. Xu, and J. Swings. 1998. Vibrios associated with Penaeus chinensis (Crustacea: Decapoda) larvae in Chinese shrimp hatcheries. Aquaculture 169:121-132.
31. Wang, B., J. Yu, Y. Li, W. Ji, and H. Xu. 2002. Isolation and identification of pathogen (Vibrio harveyi) from sea perch, Lateolabrax japonicus. J. Ocean Univ. China 9:52-55.
32. Wardlaw, A. C. 1985. Practical statistics for experimental biologists. John Wiley & Sons, Chichester, United Kingdom.
33. Yamamoto, K., Y. Ichinose, H. Shinagawa, K. Makino, A. Nakata, M. Iwanaga, T. Honda, and T. Miwatani. 1990. Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (MyA) and characterization of the processed products. Infect. Immun. 58:4106-4116.
34. Zhang, X.-H., and B. Austin. 2000. Pathogenicity of Vibrio harveyi to salmonids. J. Fish Dis. 23:93-102.
35. Zhang, X.-H., P. G. Meaden, and B. Austin. 2001. Duplication of hemolysin genes in a virulent isolate of Vibrio harveyi. Appl. Environ. Microbiol. 67:3161-3167.