Okadaic acid overcomes the blocked cell cycle caused by depleting Cdc2-related kinases in Trypanosoma brucei

Experimental Cell Research

Volumn 312, Issue 18, 2006, Pages 3504-3516

  Li, Ziyin ^a   Tu, Xiaoming ^a   Wang, Ching C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cdc2 related kinase; Cell cycle; Cytokinesis; Kinetoplast; Okadaic acid; Trypanosoma brucei

Indexed keywords

AURORA B KINASE; CYCLIN B2; CYCLIN DEPENDENT KINASE 1; FOSTRIECIN; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE 1; PHOSPHOPROTEIN PHOSPHATASE 2A; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR;

ARTICLE; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE REGULATION; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME RELEASE; KINETOPLAST; MULTINUCLEAR CELL; NONHUMAN; NUCLEAR DIVISION; PRIORITY JOURNAL; RNA INTERFERENCE; TRYPANOSOMA BRUCEI; WILD TYPE;

EUKARYOTA; TRYPANOSOMA BRUCEI;

EID: 33749118461     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.yexcr.2006.07.022     Document Type: Article

References (36)

1. Maton G., Lorca T., Girault J.-A., Ozon R., and Jessus C. Differential regulation of Cdc2 and Aurora-A in Xenopus oocytes: a crucial role of phosphatase 2A. J. Cell Sci. 118 (2005) 2485-2494
2. Mendenhall M.D., and Hodge A.E. Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62 (1998) 1191-1243
3. Morgan D.O. Cyclin-dependent kinases: engines, clocks, and microprocessors. Annu. Rev. Cell Dev. Biol. 13 (1997) 261-291
4. Skibbens R.V., and Hieter P. Kinetochores and the checkpoint mechanism that monitors for defects in the chromosome segregation machinery. Annu. Rev. Genet. 32 (1998) 307-337
5. Vickerman K. The evolutionary expansion of the trypanosomatid flagellates. Int. J. Parasitol. 24 (1994) 1317-1331
6. Stevens J.R., Noyes H.A., Dover G.A., and Gibson W.C. The ancient and divergent origins of the human pathogenic trypanosomes, Trypanosoma brucei and T. cruzi. Parasitology 118 (1999) 107-116
7. Ploubidou A., Robinson D.R., Docherty R.C., Ogbadoyi E.O., and Gull K. Evidence for novel cell cycle checkpoints in trypanosomes: kinetoplast segregation and cytokinesis in the absence of mitosis. J. Cell Sci. 112 (1999) 4641-4650
8. Li Z., and Wang C.C. A PHO80-like cyclin and a B-type cyclin control the cell cycle of the procyclic form of Trypanosoma brucei. J. Biol. Chem. 278 (2003) 20652-20658
9. Hammarton T.C., Mottram J.C., and Doerig C.D. The cell cycle of parasitic protozoa: potential for chemotherapeutic exploitation. Prog. Cell Cycle Res. 5 (2003) 91-101
10. Mottram J.C., and Smith G. A family of trypanosome cdc2-related protein kinases. Gene 162 (1995) 147-152
11. Tu X., and Wang C.C. The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion. J. Biol. Chem. 279 (2004) 20519-20528
12. Tu X., and Wang C.C. Coupling of posterior cytoskeletal morphogenesis to the G1/S transition in the Trypanosoma brucei cell cycle. Mol. Biol. Cell 16 (2005) 97-105
13. Hammarton T.C., Clark J., Douglas F., Boshart M., and Mottram J.C. Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin. J. Biol. Chem. 278 (2003) 22877-22886
14. Tu X., Kumar P., Li Z., and Wang C.C. An aurora B kinase homologue is essential for mitosis and cytokinesis in Trypanosoma brucei. J. Biol. Chem. 281 (2006) 9677-9687
15. Tu X., and Wang C.C. Pairwise knockdowns of cdc2-related kinases (CRKs) in Trypanosoma brucei identified the CRKs for G1/S and G2/M transitions and demonstrated distinctive cytokinetic regulations between two developmental stages of the organism. Eukaryotic Cell. 4 (2005) 755-764
16. Cohen P. The structure and regulation of protein phosphatases. Annu. Rev. Biochem. 58 (1989) 453-508
17. Das A., Gale Jr. M., Carter V., and Parsons M. The protein phosphatase inhibitor okadaic acid induces defects in cytokinesis and organellar genome segregation in Trypanosoma brucei. J. Cell Sci. 107 (1994) 3477-3483
18. Wirtz E., Leal S., Ochatt C., and Cross G.A. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 99 (1999) 89-101
19. Cunningham I. New culture medium for maintenance of tsetse tissues and growth of trypanosomatids. J. Protozool. 21 (1977) 325-329
20. Wang Z., Morris J.C., Drew M.E., and Englund P.T. Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem. 275 (2000) 40174-40179
21. Carruthers V.B., and Cross G.A. High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates. Proc. Natl. Acad. Sci. U. S. A. 89 (1992) 8818-8821
22. Kilmartin J.V., Wright B., and Milstein C. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93 (1982) 576-582
23. Sherwin T., Schneider A., Sasse R., Seebeck T., and Gull K. Distinct localization and cell cycle dependence of COOH terminally tyrosinolated alpha-tubulin in the microtubules of Trypanosoma brucei brucei. J. Cell Biol. 104 (1987) 439-446
24. Wehland J., Willingham M.C., and Sandoval I.V. A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo. J. Cell Biol. 97 (1983) 1467-1475
25. Woods A., Sherwin T., Sasse R., MacRae T.H., Baines A.J., and Gull K. Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies. J. Cell Sci. 93 (1989) 491-500
26. Roberge M., Tudan C., Hung S.M., Harder K.W., Jirik F.R., and Anderson H. Antitumor drug fostriecin inhibits the mitotic entry checkpoint and protein phosphatases 1 and 2A. Cancer Res. 54 (1994) 6115-6121
27. Cheng A., Balczon R., Zuo Z., Koons J.S., Walsh A.H., and Honkanen R.E. Fostriecin-mediated G2-M-phase growth arrest correlates with abnormal centrosome replication, the formation of aberrant mitotic spindles, and the inhibition of serine/threonine protein phosphatase activity. Cancer Res. 58 (1998) 3611-3619
28. Felix M.A., Cohen P., and Karsenti E. Cdc2 H1 kinase is negatively regulated by a type 2A phosphatase in the Xenopus early embryonic cell cycle: evidence from the effects of okadaic acid. EMBO J. 9 (1990) 675-683
29. Goris J., Hermann J., Hendrix P., Ozon R., and Merlevede W. Okadaic acid, a specific protein phosphatase inhibitor, induces maturation and MPF formation in Xenopus laevis oocytes. FEBS Lett. 245 (1989) 91-94
30. Clarke P.R., Hoffmann I., Draetta G., and Karsenti E. Dephosphorylation of cdc25-C by a type-2A protein phosphatase: specific regulation during the cell cycle in Xenopus egg extracts. Mol. Biol. Cell 4 (1993) 397-411
31. Karaiskou A., Jessus C., Brassac T., and Ozon R. Phosphatase 2A and polo kinase, two antagonistic regulators of cdc25 activation and MPF auto-amplification. J. Cell Sci. 112 (1999) 3747-3756
32. Abrieu A., Brassac T., Galas S., Fisher D., Labbe J.C., and Doree M. The Polo-like kinase Plx1 is a component of the MPF amplification loop at the G2/M-phase transition of the cell cycle in Xenopus eggs. J. Cell Sci. 111 (1998) 1751-1757
33. Kinoshita N., Ohkura H., and Yanagida M. Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle. Cell. 63 (1990) 405-415
34. Cohen P. The role of protein phosphorylation in human health and disease. Eur. J. Biochem. 268 (2001) 5001-5010
35. Berndt N. Roles and regulation of serine/threonine-specific protein phosphatases in the cell cycle. Prog. Cell Cycle Res. 5 (2003) 497-510
36. Liu C.W., Wang R.H., Dohadwala M., Schonthal A.H., Villa-Moruzzi E., and Berndt N. Inhibitory phosphorylation of PP1alpha catalytic subunit during the G(1)/S transition. J. Biol. Chem. 274 (1999) 29470-29475