Immobilization of UDP-galactose 4-epimerase from Escherichia coli on the yeast cell surface

Bioscience, Biotechnology and Biochemistry

Volumn 70, Issue 9, 2006, Pages 2303-2306

  Zhang, Hou Cheng ^a   Bi, Jin Yan ^a   Chen, Chang ^a   Huang, Gang Liang ^a   Qi, Qing Sheng ^a   Xiao, Min ^a   Wang, Peng George ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Cell surface; Saccharomyces cerevisiae; UDP galactose 4 epimerase

Indexed keywords

CELL SURFACE; ENZYME ACTIVITY; SACCHAROMYCES CEREVISIAE; UDP-GALACTOSE 4-EPIMERASE;

BIOCONVERSION; CELLS; ESCHERICHIA COLI; GLUCOSE; YEAST;

ENZYME IMMOBILIZATION;

BACTERIAL DNA; IMMOBILIZED ENZYME; URIDINE DIPHOSPHATE GALACTOSE; URIDINE DIPHOSPHATE GLUCOSE; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE;

ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

CLONING, MOLECULAR; DNA, BACTERIAL; ELECTROPHORESIS, CAPILLARY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; UDPGLUCOSE 4-EPIMERASE; URIDINE DIPHOSPHATE GALACTOSE; URIDINE DIPHOSPHATE GLUCOSE;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 33748994262     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.60134     Document Type: Article

References (17)

1. McAuliffe, J. C., and Hindsgaul, O., Carbohydrate drugs: an ongoing challenge. Chem. Ind., 170-174 (1997).
2. Wong, C. H., Haynie, S. L., and Whitesides, G. M., Enzyme-catalyzed synthesis of N-acetyllactosamine with in situ regeneration of uridine 5′-diphosphate glucose and uridine 5′-diphosphate galactose. J. Org. Chem., 47, 5416-5418 (1982).
3. Wong, C. H., Wang, R., and Ichikawa, Y., Regeneration of sugar nucleotide for enzymatic oligosaccharide synthesis: use of Gal-1-phosphate uridyltransferase in the regeneration of UDP-galactose, UDP-2-deoxygalactose, and UDP-galactosamine. J. Org. Chem., 57, 4343-4344 (1992).
4. Auge, C., Mathieu, C., and Merienne, C., The use of an immobilized cyclic multi-enzyme system to synthesize branched penta- and hexasaccharides associated with blood-group I epitopes. Carbohyd. Res., 151, 147-156 (1986).
5. Thiem, J., and Wiemann, T., Synthesis of galactose-terminated oligosaccharides by use of galactosyltransferase. Synthesis, 141-145 (1992).
6. Chen, X., Zhang, J., Kowal, P., Liu, Z., Andreana, P. R., Lu, Y., and Wang, P. G., Transferring a biosynthetic cycle into a productive E. coli strain: large-scale synthesis of galactosides. J. Am. Chem. Soc., 123, 8866-8867 (2001).
7. Nahalka, J., Liu, Z. Y., Chen, X., and Wang, P. G., "Superbeads, " an immobilized preparation for "Sweet." Chem. Eur. J., 9, 372-377 (2003).
8. Kondo, A., and Ueda, M., Yeast cell-surface display: applications of molecular display. Appl. Microbiol. Biotechnol., 64, 28-40 (2004).
9. Frey, P. A., The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose. FASEB J., 10, 461-470 (1996).
10. Holton, J. B., Walter, J. H., and Tyfield, L. A., "Metabolic and Molecular Bases of Inherited Disease" 8th ed., eds. Scriver, C. R., Beaudet, A. L., Sly, S. W., and Valle, D., McGraw Hill, New York, pp. 1553-1587 (2000).
11. Holden, H. M., Rayment, I., and Thoden, J. B., Structure and function of enzymes of the Leloir pathway for galactose metabolism. J. Biol. Chem., 278, 43885-43888 (2003).
12. Chen, X., Kowal, P., Hamad, S., Fan, H., and Wang, P. G., Cloning, expression and characterization of a UDP-galactose 4-epimerase from Escherichia coli. Biotech. Lett., 21, 1131-1135 (1999).
13. Thoden, J. B., Frey, P. A., and Holden, H. M., Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli. Biochem., 35, 2557-2566 (1996).
14. Thoden, J. B., Frey, P. A., and Holden, H. M., Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: implications for the catalytic mechanism. Biochem., 35, 5137-5144 (1996).
15. Thoden, J. B., Frey, P. A., and Holden, H. M., High resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol. Protein Sci., 5, 2149-2161 (1996).
16. Brahma, A., and Bhattacharyya, D., UDP-galactose 4-epimerase from Kluyveromyces fragilis: existence of subunit independent functional site. FEBS Lett., 577, 27-34 (2004).
17. Nayar, S., and Bhattacharyya, D., UDP-galactose 4-epimerase from Escherichia coli: existence of a catalytic monomer. FEBS Lett., 409, 449-451 (1997).