Diagnostic immunohistochemistry: What can go wrong?

Advances in Anatomic Pathology

Volumn 13, Issue 5, 2006, Pages 238-246

  Yaziji, Hadi ^a   Barry, Todd ^b  

^a Ancillary Pathways   (United States)

^b PHENOPATH LABORATORIES   (United States)

Author keywords

Antibodies; Antigen retrieval; Antigens; Detection systems; Endogenous biotin; False negative; False positive; HIER; Immunohistochemistry; Immunophenotype; Immunoreactivity; Pitfalls

Indexed keywords

AVIDIN; BETA CATENIN; BIOTIN; CD20 ANTIGEN; CD23 ANTIGEN; CD34 ANTIGEN; CD99 ANTIGEN; COMPLEMENT COMPONENT C3B RECEPTOR; COMPLEMENT COMPONENT C3D RECEPTOR; CYCLIN D1; CYTOKERATIN 20; EPIDERMAL GROWTH FACTOR RECEPTOR; FORMALDEHYDE; GLYCOPROTEIN GP 100; INTERLEUKIN 2 RECEPTOR ALPHA; KERATIN; KI 67 ANTIGEN; LEUKOSIALIN; MYELOPEROXIDASE; PEROXIDASE; PROGESTERONE RECEPTOR; PROTEIN P53; PROTEIN P57; PROTEIN S 100; SMOOTH MUSCLE ACTIN; STEM CELL FACTOR RECEPTOR; SYNAPTOPHYSIN; TRANSCRIPTION FACTOR CDX2; UNINDEXED DRUG; VIMENTIN;

ALGORITHM; CARCINOMA; DIAGNOSTIC ERROR; DIAGNOSTIC PROCEDURE; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; HUMAN; IMMUNOHISTOCHEMISTRY; IMMUNOPHENOTYPING; IMMUNOREACTIVITY; INSTRUMENTATION; LYMPHOMA; MELANOMA; PATHOLOGICAL ANATOMY; PRIORITY JOURNAL; REVIEW; SARCOMA; SURGICAL PATHOLOGY; TISSUE FIXATION; TISSUE PREPARATION;

ALGORITHMS; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HUMANS; IMMUNOHISTOCHEMISTRY; PATHOLOGY, SURGICAL; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION;

EID: 33748956895     PISSN: 10724109     EISSN: 15334031     Source Type: Journal    
DOI: 10.1097/01.pap.0000213041.39070.2f     Document Type: Review

References (60)

1. Chen CC, Raikow RB, Sonmez-Alpan E, et al. Classification of small B-cell lymphoid neoplasms using a paraffin section immunohistochemical panel. Appl Immunohistochem Mol Morphol. 2000;8:1-11.
2. Tubbs RR, Hsi ED, Hicks D, et al. Molecular pathology testing of tissues fixed in prefer solution. Am J Surg Pathol. 2004;28:417-419.
3. Poston RN. A new look at the original cases of Hodgkin's disease. Cancer Treat Rev. 1999;25:151-155.
4. Fox CH, Johnson FB, Whiting J, et al. Formaldehyde fixation. J Histochem Cytochem. 1985;33:845-853.
5. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981;29:577-580.
6. Sabattini E, Bisgaard K, Ascani S, et al. The EnVision++ system: a new immunohistochemical method for diagnostics and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC techniques. J Clin Pathol. 1998;51:506-511.
7. Kammerer U, Kapp M, Gassel AM, et al. A new rapid immunohistochemical staining technique using the EnVision antibody complex. J Histochem Cytochem. 2001;49:623-630.
8. Adams JC. Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem. 1992;40:1457-1463.
9. Werner M, Chott A, Fabiano A, et al. Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol. 2000;24:1016-1019.
10. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 1991;39:741-748.
11. Cattoretti G, Becker MH, Key G, et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol. 1992;168:357-363.
12. Gown AM, de Wever N, Battifora H. Microwave-based antigenic unmasking: a revolutionary new technique for routine immunohistochemistry. Appl Immunohistochem. 1993;1:256-266.
13. Cattoretti G, Pileri S, Parravicini C, et al. Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections [see comments]. J Pathol. 1993;171:83-98.
14. Cuevas EC, Bateman AC, Wilkins BS, et al. Microwave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J Clin Pathol. 1994;47:448-452.
15. Bankfalvi A, Navabi H, Bier B, et al. Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry. J Pathol. 1994;174:223-228.
16. Norton AJ, Jordan S, Yeomans P. Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J Pathol. 1994;173:371-379.
17. Taylor CR, Shi SR, Chen C, et al. Comparative study of antigen retrieval heating methods: microwave, microwave and pressure cooker, autoclave, and steamer. Biotech Histochem. 1996;71:263-270.
18. Shi SR, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry: past, present, and future. J Histochem Cytochem. 1997;45:327-343.
19. Iyer PV, Leong AS. Poorly differentiated squamous cell carcinomas of the skin can express vimentin. J Cutan Pathol. 1992;19:34-39.
20. Domagala W, Lasota J, Bartkowiak J, et al. Vimentin is preferentially expressed in human breast carcinomas with low estrogen receptor and high Ki-67 growth fraction. Am J Pathol. 1990;136:219-227.
21. Gustafsson H, Virtanen I, Thornell LE. Expression of cytokeratins and vimentin in salivary gland carcinomas as revealed with monoclonal antibodies. Virchows Arch A Pathol Anat Histopathol. 1988;412:515-524.
22. Dabbs DJ, Geisinger KR, Norris HT. Intermediate filaments in endometrial and endocervical carcinomas. The diagnostic utility of vimentin patterns. Am J Surg Pathol. 1986;10:568-576.
23. Gustmann C, Altmannsberger M, Osborn M, et al. Cytokeratin expression and vimentin content in large cell anaplastic lymphomas and other non-Hodgkin's lymphomas. Am J Pathol. 1991;138:1413-1422.
24. Pinkus GS, Etheridge CL, O'Connor EM. Are keratin proteins a better tumor marker than epithelial membrane antigen? A comparative immunohistochemical study of various paraffin-embedded neoplasms using monoclonal and polyclonal antibodies. Am J Clin Pathol. 1986;85:269-277.
25. Eusebi V, Carcangiu ML, Dina R, et al. Keratin-positive epithelioid angiosarcoma of thyroid. A report of four cases. Am J Surg Pathol. 1990;14:737-747.
26. Miettinen M. Keratin subsets in spindle cell sarcomas. Keratins are widespread but synovial sarcoma contains a distinctive keratin polypeptide pattern and desmoplakins. Am J Pathol. 1991;138:505-513.
27. Corson JM. Keratin protein immunohistochemistry in surgical pathology practice. Pathol Annu. 1986;21:47-81.
28. Miettinen M, Virtanen I, Damjanov H. Coexpression of keratin and vimentin in epithelioid sarcoma. Am J Surg Pathol. 1985;9:460-463.
29. Miettinen M, Lehto VP, Virtanen I. Keratin in the epithelial-like cells of classical biphasic synovial sarcoma. Virchows Arch B Cell Pathol Incl Mol Pathol. 1982;40:157-161.
30. Miettinen M, Franssila K. Immunohistochemical spectrum of malignant melanoma. The common presence of keratins. Lab Invest. 1989;61:623-628.
31. Leong AS-Y, Vinyuvat S, Suthipintawong C, et al. Patterns of basal lamina immunostaining in soft-tissue and bony tumors. Appl Immunohistochem. 1997;5:1-7.
32. Menasce LP, Banerjee SS, Beckett E, et al. Extra-medullary myeloid tumour (granulocytic sarcoma) is often misdiagnosed: a study of 26 cases. Histopathology. 1999;34:391-398.
33. Trupiano JK, Geisinger KR, Willingham MC, et al. Diffuse malignant mesothelioma of the peritoneum and pleura, analysis of markers. Mod Pathol. 2004;17:476-481.
34. Ordonez NG. Podoplanin: a novel diagnostic immunohistochemical marker. Adv Anat Pathol. 2006;13:83-88.
35. Roberts F, Harper CM, Downie I, et al. Immunohistochemical analysis still has a limited role in the diagnosis of malignant mesothelioma. A study of thirteen antibodies. Am J Clin Pathol. 2001;116:253-262.
36. Hwang H, Quenneville L, Yaziji H, et al. Wilms tumor gene product: sensitive and contextually specific marker of serous carcinomas of ovarian surface epithelial origin. Appl Immunohistochem Mol Morphol. 2004;12:122-126.
37. Werling RW, Yaziji H, Bacchi CE, et al. CDX2, a highly sensitive and specific marker of adenocarcinomas of intestinal origin: an immunohistochemical survey of 476 primary and metastatic carcinomas. Am J Surg Pathol. 2003;27:303-310.
38. Goldstein NS, Thomas M. Mucinous and nonmucinous bronchioloalveolar adenocarcinomas have distinct staining patterns with thyroid transcription factor and cytokeratin 20 antibodies. Am J Clin Pathol. 2001;116:319-325.
39. Zarbo RJ, Gown AM, Nagle RB, et al. Anomalous cytokeratin expression in malignant melanoma: one- and two-dimensional western blot analysis and immunohistochemical survey of 100 melanomas. Mod Pathol. 1990;3:494-501.
40. Banks ER, Jansen JF, Oberle E, et al. Cytokeratin positivity in fine-needle aspirates of melanomas and sarcomas [see comments]. Diagn Cytopathol. 1995;12:230-233.
41. Battifora H. Misuse of the term 'expression' [letter; comment]. Am J Clin Pathol. 1989;92:708-709.
42. Swanson PE. Heffalumps, jagulars, and cheshire cats. A commentary on cytokeratins and soft tissue sarcomas [see comments]. Am J Clin Pathol. 1991;95:S2-S7.
43. Swanson PE. HIERanarchy: the state of the art in immunohistochemistry [editorial]. Am J Clin Pathol. 1997;107:139-140.
44. Bacchi CE, Zarbo RJ, Jiang JJ, et al. Do glioma cells express cytokeratin? Appl Immunohistochem. 1995;3:45-53.
45. Harvey JM, Clark GM, Osborne CK, et al. Estrogen receptor status by immunohistochemistry is superior to the ligand-binding assay for predicting response to adjuvant endocrine therapy in breast cancer. J Clin Oncol. 1999;17:1474-1481.
46. Bardou VJ, Arpino G, Elledge RM, et al. Progesterone receptor status significantly improves outcome prediction over estrogen receptor status alone for adjuvant endocrine therapy in two large breast cancer databases. J Clin Oncol. 2003;21:1973-1979.
47. Bonds LA, Barnes P, Foucar K, et al. Acetic Acid-zinc-formalin: a safe alternative to B-5 fixative. Am J Clin Pathol. 2005;124:205-211.
48. Matthews JB, Mason GI. Influence of decalcifying agents on immunoreactivity of formalin-fixed, paraffin-embedded tissue. Histochem J. 1984;16:771-787.
49. Athanasou NA, Quinn J, Heryet A, et al. Effect of decalcification agents on immunoreactivity of cellular antigens. J Clin Pathol. 1987;40:874-878.
50. Almasri NM, Duque RE, Iturraspe J, et al. Reduced expression of CD20 antigen as a characteristic marker for chronic lymphocytic leukemia. Am J Hematol. 1992;40:259-263.
51. Wiestner A, Rosenwald A, Barry TS, et al. ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood. 2003;101:4944-4951.
52. Cragg MS, Bayne MC, Illidge TM, et al. Apparent modulation of CD20 by rituximab: an alternative explanation. Blood. 2004;103:3989-3990. Author reply 3990-3991.
53. Barry TS, Jaffe ES, Sorbara L, et al. Peripheral T-cell lymphomas expressing CD30 and CD15. Am J Surg Pathol. 2003;27:1513-1522.
54. Yao X, Teruya-Feldstein J, Raffeld M, et al. Peripheral T-cell lymphoma with aberrant expression of CD79a and CD20: a diagnostic pitfall. Mod Pathol. 2001;14:105-110.
55. Lai R, Weiss LM, Chang KL, et al. Frequency of CD43 expression in non-Hodgkin lymphoma. A survey of 742 cases and further characterization of rare CD43+ follicular lymphomas. Am J Clin Pathol. 1999;111:488-494.
56. Marshall-Taylor CE, Cartun RW, Mandich D, et al. Immunohistochemical detection of immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas using formalin-fixed, paraffinembedded tissues and a heat-induced epitope retrieval technique. Appl Immunohistochem Mol Morphol. 2002;10:258-262.
57. Du MQ, Liu H, Diss TC, et al. Kaposi sarcoma-associated herpesvirus infects monotypic (IgM lambda) but polyclonal naive B cells in Castleman disease and associated lymphoproliferative disorders. Blood. 2001;97:2130-2136.
58. Attygalle AD, Liu H, Shirali S, et al. Atypical marginal zone hyperplasia of mucosa-associated lymphoid tissue: a reactive condition of childhood showing immunoglobulin lambda light-chain restriction. Blood. 2004;104:3343-3348.
59. Brousset P, Butet V, Chittal S, et al. Comparison of in situ hybridization using different nonisotopic probes for detection of Epstein-Barr virus in nasopharyngeal carcinoma and immunohistochemical correlation with anti-latent membrane protein antibody. Lab Invest. 1992;67:457-464.
60. Murono S, Yoshizaki T, Tanaka S, et al. Detection of Epstein-Barr virus in nasopharyngeal carcinoma by in situ hybridization and polymerase chain reaction. Laryngoscope. 1997;107:523-526.