Characterization and Sequence Verification of Thiolated Deoxyoligonucleotides Used for Microarray Construction

Journal of the American Society for Mass Spectrometry

Volumn 17, Issue 10, 2006, Pages 1397-1401

  Van Aerschot, Arthur ^a   Rozenski, Jef ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLICS; DNA; IMPURITIES; LIQUID CHROMATOGRAPHY; OPTIMIZATION; SYNTHESIS (CHEMICAL);

ACRYLAMIDE DERIVATIZATION; MICROCHIPS; SULFINIC ACID; SYNTHESIS PROTOCOLS;

OLIGOMERS;

ACRYLAMIDE; ION; OLIGODEOXYNUCLEOTIDE; OLIGONUCLEOTIDE; SULFINIC ACID DERIVATIVE; THIOL DERIVATIVE;

ARTICLE; DNA MICROARRAY; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICROARRAY ANALYSIS; MOLECULE; OXIDATION; REACTION ANALYSIS; SYNTHESIS;

EID: 33748955961     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2006.07.011     Document Type: Article

References (18)

1. Okamoto T., Suzuki T., and Yamamoto N. Microarray Fabrication with Covalent Attachment of DNA Using Bubble Jet Technology. Nature Biotech 18 (2000) 438-441
2. Yu F., Yao D., and Knoll W. Oligonucleotide Hybridization Studied by a Surface Plasmon Diffraction Sensor (SPDS). Nucleic Acids Res (2004) 32e75
3. Fixe F., Dufva M., Telleman P., and Christensen C.B.V. One-Step Immobilization of Aminated and Thiolated DNA Onto Poly(Methylmethacrylate) (PMMA) Substrates. Lab. Chip 4 (2004) 191-195
4. Tuukanen S., Virtanen J., Hytönen V.P., Kulomaa M.S., and Törma P. Fabrication of DNA Monolayers on Gold Substrates and Guiding of DNA with Electric Field. Rev. Adv. Mater. Sci 5 (2003) 228-231
5. Demers L.M., Ginger D.S., Park S.-J., Li Z., Chung S.-W., and Mirkindagger C.A. Direct Patterning of Modified Oligonucleotides on Metals and Insulators by Dip-Pen Nanolithography. Science 296 (2002) 1836-1838
6. Sproat B.S., Beijer B., Rider P., and Neuner P. The Synthesis of Protected 5′Mercapto-2′,5′-Dideoxyribonucleoside-3′-O-Phosphoramidites; Uses of 5′-Mercapto-Oligoribonucleosides. Nucleic Acids Res 15 (1987) 4837-4848
7. Connolly B.A., and Rider P. Chemical Synthesis of Oligonucleotide Containing a Free Sulphydryl Group and Subsequent Attachment of Thiol Specific Probes. Nucleic Acids Res 13 (1985) 4485-4502
8. Gaur R.K., Sharma P., and Gupta K.C. A Simple Method for the Introduction of Thiol Group at 5′-Termini of Oligodeoxynucleotides. Nucleic Acids Res 17 (1989) 4404
9. Gao H., Yang M., Patel R., and Cook A.F. Circularization of Oligonucleotides by Disulfide Bridge Formation. Nucleic Acids Res 23 (1995) 2025-2029
10. Gao H., Yang M., Patel R., and Cook A.F. Stabilization of Double-Stranded Oligonucleotides Using Backbone-Linked Disulfide Bridges. Nucleic Acids Res 23 (1995) 285-292
11. Apffel A., Chakel J.A., Fischer S., Lichtenwalter K., and Hancock W.S. Analysis of Oligonucleotides by HPLC-Electrospray Ionization Mass Spectrometry. Anal. Chem 69 (1997) 1320-1325
12. Sinha N.D., and Cook R.M. The Preparation and Application of Functionalized Synthetic Oligonucleotides. III. Use of H-Phosphonate Derivatives of Protected Amino-Hexanol and Mercapto-Propanol or -Hexanol. Nucleic Acids Res 16 (1988) 1659-1669
13. Kumar A., Advani S., Dawar H., and Talwar G.P. A Simple Method for Introducing a Thiol Group at the 5′-End of Synthetic Oligonucleotides. Nucleic Acids Res 19 (1991) 4561
14. Rozenski J., and McCloskey J.A. SOS: A Simple Interactive Program for ab Initio Oligonucleotide Sequencing by Mass Spectrometry. J. Am. Soc. Mass Spectrom 13 (2002) 200-203
15. McLuckey S.A., Van Berkel G.J., and Glish G.L. Tandem Mass Spectrometry of Small, Multiply-Charged Oligonucleotides. J. Am. Soc. Mass Spectrom 3 (1992) 60-70
16. Rozenski J., and McCloskey J.A. Determination of Nearest Neighbors in Nucleic Acids by Mass Spectrometry. Anal. Chem 71 (1999) 1454-1459
17. Garzitto M., Rovatto L., and Hamdan M. On-Line Liquid Chromatography/Electrospray Tandem Mass Spectrometry to Investigate Acrylamide Adducts with Cysteine Residues: Implications for Polyacrylamide Gel Electrophoresis Separations of Proteins. Rapid Commun. Mass Spectrom 12 (1998) 484-488
18. Houde D., Kauppinen P., Mhatre R., and Lyubarskaya Y. Determination of Protein Oxidation by Mass Spectrometry and Method Transfer to Quality Control. J. Chromatorgr 1123 (2006) 189-198