A novel method for estimating viable Salmonella cell counts using real-time PCR

Journal of the Food Hygienic Society of Japan

Volumn 47, Issue 4, 2006, Pages 151-156

  Fujikawa, Hiroshi ^a   Shimojima, Yukako ^a   Yano, Kazuyoshi ^a  

^a TOKYO METROPOLITAN INSTITUTE OF PUBLIC HEALTH   (Japan)

Author keywords

Estimation method; Real time PCR; Salmonella; Threshold cycle; Viable cell counts

Indexed keywords

NUCLEASE; PEPTONE; WATER;

ALGORITHM; ARTICLE; BACTERIAL CELL; CELL COUNT; CELL DEATH; CELL VIABILITY; CULTURE MEDIUM; FOOD; GENE TARGETING; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA ENTERITIDIS; TARGET CELL; TEMPERATURE;

COLONY COUNT, MICROBIAL; COMPUTER SYSTEMS; CULTURE MEDIA; DNA, BACTERIAL; FOOD CONTAMINATION; FOOD MICROBIOLOGY; POLYMERASE CHAIN REACTION; SALMONELLA ENTERITIDIS;

BACTERIA (MICROORGANISMS); SALMONELLA; SALMONELLA ENTERITIDIS;

EID: 33748931437     PISSN: 00156426     EISSN: None     Source Type: Journal    
DOI: 10.3358/shokueishi.47.151     Document Type: Article

References (13)

1. Hanna, E., Scott, C., Connor, J., Wang, H.H., Real-time polymerase chain reaction for the food microbiologist: technologies, applications, and limitations. J. Food Sci., 70, R49-R53 (2005).
2. Bassler, H. A., Flood, S. J. A., Livak, K. J., Marmaro, J., Knorr, R., Batt, C. A., Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Appl. Environ. Microbiol., 61, 3,24-3,728 (1995).
3. Lyon, W. J., TaqMan PCR for detection of Vibrio cholerae O1, O139, non-O1, and non-O139 in pure cultures, raw oysters, and synthetic seawater. Appl. Environ. Microbiol., 67, 4,685-4,693 (2001).
4. Malorny, B., Paccassoni, E., Fach, P., Bunge, C., Martin, A., Helmuth, R., Diagnostic real-time PCR for detection of Salmonella in food. Appl. Environ. Microbiol., 70, 7,046-7,052 (2004).
5. Hsu, C.-F., Tsai, T.-U., Pan, T.-M., Use of the duplex TaqMan PCR system for detection of Shiga-like toxin-producing Escherichia coli. J. Clin. Microbiol., 43, 2,668-2,673 (2005).
6. Josefsen, M. H., Jacobsen, N. R., Hoofar, J., Enrichment followed by quantitative PCR both for rapid detection and as a tool for quantitative risk assessment of foodborne thermotolerant Campylobacter. Appl. Environ. Microbiol., 70, 3,588-3,592 (2004).
7. Knutsson, R., Lofstrom, C., Grage, H., Hoorfar, J., Radstrom, P., Modeling of 5′-nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica. J. Clin. Microbiol., 40, 52-60 (2002).
8. Fujikawa, H., Morozumi, S., New estimation methods of bacterial concentration by measuring ATP changes during incubation. J. Food Hyg. Japan, 43, 155-159 (2002).
9. Fujikawa, H., Morozumi, S., Estimation of bacterial concentrations in commercial foods by measuring ATP changes during incubation. J. Food Hyg. Japan, 44, 83-88 (2003).
10. Rolfs, A., I., Schuller, I., Finckt, U., Weber-Rolfs, I., "PCR: Clinical Diagnostics and Research", Berlin, Springer-Verlag, 1992.
11. Hoorfar, J., Ahrens, P., Radstrom, P., Automated 5′-nuclease PCR assay for identification of Salmonella enterica. J. Clin. Microbiol., 38, 3,429-3,435 (2000).
12. Galan, J. E., Ginocchio, C., Costeas, P., Molecular and functional characterization of the Salmonella invasion gene invA: homology of InvA to members of a new protein family. J. Bacteriol., 174, 4,338-4,349 (1992).
13. Sakazaki, R. ed., "Shin Saikinbaitigakukoza Ge-II", 2nd Ed., Tokyo, Kindai Shuppan, 1990, p. 104-106. (ISBN-4-87402-468-8)