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Volumn 140, Issue 1, 2006, Pages 121-133

Mutational and functional analysis of the cryptic N-terminal targeting signal for both mitochondria and peroxisomes in yeast peroxisomal citrate synthase Cit2p

Author keywords

Citrate synthase (Cit2p); Cryptic targeting signal; Green fluorescent protein; Mitochondria; N terminal segment; Peroxisomes

Indexed keywords

ALANINE; AMINO ACID; ASPARAGINE; CITRATE SYNTHASE; GLUTAMIC ACID; LEUCINE; PROTEIN CIT 2P; UNCLASSIFIED DRUG; VALINE;

EID: 33748924293     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj136     Document Type: Article
Times cited : (7)

References (40)
  • 2
    • 0025320713 scopus 로고
    • Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver
    • Oda, T., Funai, T., and Ichiyama, A. (1990) Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver. J. Biol. Chem. 265, 7513-7519
    • (1990) J. Biol. Chem. , vol.265 , pp. 7513-7519
    • Oda, T.1    Funai, T.2    Ichiyama, A.3
  • 3
    • 0035813120 scopus 로고    scopus 로고
    • A single gene produces mitochondrial, cytoplasmic, and peroxisomal NADP-dependent isocitrate dehydrogenase in Aspergillus nidulans
    • Szewczyk, E., Andrianopoulos, A., Davis, M.A., and Hynes, M.J. (2001) A single gene produces mitochondrial, cytoplasmic, and peroxisomal NADP-dependent isocitrate dehydrogenase in Aspergillus nidulans. J. Biol. Chem. 276, 37722-37729
    • (2001) J. Biol. Chem. , vol.276 , pp. 37722-37729
    • Szewczyk, E.1    Andrianopoulos, A.2    Davis, M.A.3    Hynes, M.J.4
  • 4
    • 0021474827 scopus 로고
    • Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method
    • Suissa, M., Suda, K., and Schatz, G. (1984) Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method. EMBO J. 3, 1773-1781
    • (1984) EMBO J. , vol.3 , pp. 1773-1781
    • Suissa, M.1    Suda, K.2    Schatz, G.3
  • 5
    • 0023021130 scopus 로고
    • Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes
    • Rosenkrantz, M., Alam, T., Kim, K.S., Clark, B.J., Srere, P.A., and Guarente, L.P. (1986) Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes. Mol. Cell. Biol. 6, 4509-1515
    • (1986) Mol. Cell. Biol. , vol.6 , pp. 4509-11515
    • Rosenkrantz, M.1    Alam, T.2    Kim, K.S.3    Clark, B.J.4    Srere, P.A.5    Guarente, L.P.6
  • 6
    • 0030994770 scopus 로고    scopus 로고
    • The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase
    • Jia, Y.K., Becam, A.M., and Herbert, C.J. (1997) The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase. Mol. Microbiol. 24, 53-59
    • (1997) Mol. Microbiol. , vol.24 , pp. 53-59
    • Jia, Y.K.1    Becam, A.M.2    Herbert, C.J.3
  • 7
    • 0025247671 scopus 로고
    • Citrate synthase encoded by the CIT2 gene of Saccharomyces cerevisiae is peroxisomal
    • Lewin, A.S., Hines, V., and Small, G.M. (1990) Citrate synthase encoded by the CIT2 gene of Saccharomyces cerevisiae is peroxisomal. Mol. Cell. Biol. 10, 1399-1405
    • (1990) Mol. Cell. Biol. , vol.10 , pp. 1399-1405
    • Lewin, A.S.1    Hines, V.2    Small, G.M.3
  • 8
    • 0006694604 scopus 로고
    • Purification and characterization of nonmitochondrial citrate synthase from Saccharomyces cerevisiae
    • Cho, N.S., Kim, K.S., and Maeng, P.J. (1991) Purification and characterization of nonmitochondrial citrate synthase from Saccharomyces cerevisiae. Kor. J. Microbiol. 29, 230-237
    • (1991) Kor. J. Microbiol. , vol.29 , pp. 230-237
    • Cho, N.S.1    Kim, K.S.2    Maeng, P.J.3
  • 9
    • 0006741816 scopus 로고
    • Organelle targeting of citrate synthases in Saccharomyces cerevisiae
    • Lee, H.S., Choi, W.S., Lee, J.S., and Maeng, P.J. (1994) Organelle targeting of citrate synthases in Saccharomyces cerevisiae. Kor. J. Microbiol. 32, 558-564
    • (1994) Kor. J. Microbiol. , vol.32 , pp. 558-564
    • Lee, H.S.1    Choi, W.S.2    Lee, J.S.3    Maeng, P.J.4
  • 10
    • 0034533501 scopus 로고    scopus 로고
    • Identification of a cryptic N-terminal signal in Saccharomyces cerevisiae peroxisomal citrate synthase that functions in both peroxisomal and mitochondrial targeting
    • Lee, J.G., Cho, S.P., Lee, H.S., Lee, C.H., Bae, K.S., and Maeng, P.J. (2000) Identification of a cryptic N-terminal signal in Saccharomyces cerevisiae peroxisomal citrate synthase that functions in both peroxisomal and mitochondrial targeting. J. Biochem. 128, 1059-1072
    • (2000) J. Biochem. , vol.128 , pp. 1059-1072
    • Lee, J.G.1    Cho, S.P.2    Lee, H.S.3    Lee, C.H.4    Bae, K.S.5    Maeng, P.J.6
  • 11
    • 0020529962 scopus 로고
    • Transformation of intact yeast cells treated with alkali cations
    • Ito, H., Fukuda, Y., Murata, K., and Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168
    • (1983) J. Bacteriol. , vol.153 , pp. 163-168
    • Ito, H.1    Fukuda, Y.2    Murata, K.3    Kimura, A.4
  • 12
    • 0004136246 scopus 로고    scopus 로고
    • (Irwin, N. and Janssen, K.A., eds.) 3 vols., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
    • Sambrook, J. and Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual. 3 ed. (Irwin, N. and Janssen, K.A., eds.) 3 vols., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
    • (2001) Molecular Cloning: A Laboratory Manual. 3 Ed.
    • Sambrook, J.1    Russell, D.W.2
  • 13
    • 77957010982 scopus 로고
    • Citrate synthase
    • Srere, P.A. (1969) Citrate synthase. Methods Enzymol. 13, 3-26
    • (1969) Methods Enzymol. , vol.13 , pp. 3-26
    • Srere, P.A.1
  • 14
    • 0022731046 scopus 로고
    • Saccharomyces cerevisiae contains two functional citrate synthase genes
    • Kim, K.S., Rosenkrantz, M.S., and Guarente, L. (1986) Saccharomyces cerevisiae contains two functional citrate synthase genes. Mol. Cell. Biol. 6, 1936-1942
    • (1986) Mol. Cell. Biol. , vol.6 , pp. 1936-1942
    • Kim, K.S.1    Rosenkrantz, M.S.2    Guarente, L.3
  • 15
    • 0040884299 scopus 로고    scopus 로고
    • Metabolic effects of mislocalized mitochondrial and peroxisomal citrate syntheses in yeast Saccharomyces cerevisiae
    • Velot, C., Lebreton, S., Morgunov, I., Usher, K.C., and Srere, P.A. (1999) Metabolic effects of mislocalized mitochondrial and peroxisomal citrate syntheses in yeast Saccharomyces cerevisiae. Biochemistry 38, 16195-16204
    • (1999) Biochemistry , vol.38 , pp. 16195-16204
    • Velot, C.1    Lebreton, S.2    Morgunov, I.3    Usher, K.C.4    Srere, P.A.5
  • 16
    • 0023920458 scopus 로고
    • Mitochondrial presequences
    • Roise, D. and Schatz, G. (1988) Mitochondrial presequences. J. Biol. Chem. 263, 4509-4511
    • (1988) J. Biol. Chem. , vol.263 , pp. 4509-4511
    • Roise, D.1    Schatz, G.2
  • 18
    • 0022725788 scopus 로고
    • A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers
    • Roise, D., Horvath, S.J., Tomich, J.M., Richards, J.H., and Schatz, G. (1986) A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers. EMBO J. 5, 1327-1334
    • (1986) EMBO J. , vol.5 , pp. 1327-1334
    • Roise, D.1    Horvath, S.J.2    Tomich, J.M.3    Richards, J.H.4    Schatz, G.5
  • 19
    • 0030769419 scopus 로고    scopus 로고
    • Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70
    • Brix, J., Dietmeier, K., and Pfanner, N. (1997) Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70. J. Biol. Chem. 272, 20730-20735
    • (1997) J. Biol. Chem. , vol.272 , pp. 20730-20735
    • Brix, J.1    Dietmeier, K.2    Pfanner, N.3
  • 20
    • 0040610676 scopus 로고    scopus 로고
    • Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable pre-protein
    • Brix, J., Rudiger, S., Bukau, B., Schneider-Mergener, J., and Pfanner, N. (1999) Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable pre-protein. J. Biol. Chem. 274, 16522-16530
    • (1999) J. Biol. Chem. , vol.274 , pp. 16522-16530
    • Brix, J.1    Rudiger, S.2    Bukau, B.3    Schneider-Mergener, J.4    Pfanner, N.5
  • 21
    • 0029070886 scopus 로고
    • The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system
    • Berthold, J., Bauer, M.F., Schneider, H.C., Klaus, C., Dietmeier, K., Neupert, W., and Brunner, M. (1995) The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell 81, 1085-1093
    • (1995) Cell , vol.81 , pp. 1085-1093
    • Berthold, J.1    Bauer, M.F.2    Schneider, H.C.3    Klaus, C.4    Dietmeier, K.5    Neupert, W.6    Brunner, M.7
  • 22
    • 0029052947 scopus 로고
    • Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria
    • Horst, M., Hilfiker-Rothenfluh, S., Oppliger, W., and Schatz, G. (1995) Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria. EMBO J. 14, 2293-2297
    • (1995) EMBO J. , vol.14 , pp. 2293-2297
    • Horst, M.1    Hilfiker-Rothenfluh, S.2    Oppliger, W.3    Schatz, G.4
  • 23
    • 0028556615 scopus 로고
    • Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane
    • Kronidou, N.G., Oppliger, W., Bolliger, L., Hannavy, K., Glick, B.S., Schatz, G., and Horst, M. (1994) Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane. Proc. Natl. Acad. Sci. USA 91, 12818-12822
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 12818-12822
    • Kronidou, N.G.1    Oppliger, W.2    Bolliger, L.3    Hannavy, K.4    Glick, B.S.5    Schatz, G.6    Horst, M.7
  • 25
    • 0034598904 scopus 로고    scopus 로고
    • Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20
    • Abe, Y., Shodai, T., Muto, T., Mihara, K., Torii, H., Nishikawa, S., Endo, T., and Kohda, D. (2000) Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20. Cell 100, 551-560
    • (2000) Cell , vol.100 , pp. 551-560
    • Abe, Y.1    Shodai, T.2    Muto, T.3    Mihara, K.4    Torii, H.5    Nishikawa, S.6    Endo, T.7    Kohda, D.8
  • 26
    • 0035895431 scopus 로고    scopus 로고
    • NMR identification of the Tom20 binding segment in mitochondrial presequences
    • Muto, T., Obita, T., Abe, Y., Shodai, T., Endo, T., and Kohda, D. (2001) NMR identification of the Tom20 binding segment in mitochondrial presequences. J. Mol. Biol. 306, 137-143
    • (2001) J. Mol. Biol. , vol.306 , pp. 137-143
    • Muto, T.1    Obita, T.2    Abe, Y.3    Shodai, T.4    Endo, T.5    Kohda, D.6
  • 27
    • 0037466277 scopus 로고    scopus 로고
    • Peptide library approach with a disulfide tether to refine the Tom20 recognition motif in mitochondrial presequences
    • Obita, T., Muto, T., Endo, T., and Kohda, D. (2003) Peptide library approach with a disulfide tether to refine the Tom20 recognition motif in mitochondrial presequences. J. Mol. Biol. 328, 495-504
    • (2003) J. Mol. Biol. , vol.328 , pp. 495-504
    • Obita, T.1    Muto, T.2    Endo, T.3    Kohda, D.4
  • 28
    • 0032486364 scopus 로고    scopus 로고
    • Cloning and characterization of the gene encoding the human peroxisomal assembly protein Pex3p
    • Kammerer, S., Holzinger, A., Welsch, U., and Roscher, A.A. (1998) Cloning and characterization of the gene encoding the human peroxisomal assembly protein Pex3p. FEBS Lett. 429, 53-60
    • (1998) FEBS Lett. , vol.429 , pp. 53-60
    • Kammerer, S.1    Holzinger, A.2    Welsch, U.3    Roscher, A.A.4
  • 29
    • 0034122564 scopus 로고    scopus 로고
    • The peroxin pex3p initiates membrane assembly in peroxisome biogenesis
    • Ghaedi, K., Tamura, S., Okumoto, K., Matsuzono, Y., and Fujiki, Y. (2000) The peroxin pex3p initiates membrane assembly in peroxisome biogenesis. Mol. Biol. Cell 11, 2085-2102
    • (2000) Mol. Biol. Cell , vol.11 , pp. 2085-2102
    • Ghaedi, K.1    Tamura, S.2    Okumoto, K.3    Matsuzono, Y.4    Fujiki, Y.5
  • 30
    • 0034615921 scopus 로고    scopus 로고
    • A stretch of positively charged amino acids at the N terminus of Hansenula polymorpha Pex3p is involved in incorporation of the protein into the peroxisomal membrane
    • Baerends, R.J., Faber, K.N., Kram, A.M., Kiel, J.A., van der Klei, I.J., and Veenhuis, M. (2000) A stretch of positively charged amino acids at the N terminus of Hansenula polymorpha Pex3p is involved in incorporation of the protein into the peroxisomal membrane. J. Biol. Chem. 275, 9986-9995
    • (2000) J. Biol. Chem. , vol.275 , pp. 9986-9995
    • Baerends, R.J.1    Faber, K.N.2    Kram, A.M.3    Kiel, J.A.4    Van Der Klei, I.J.5    Veenhuis, M.6
  • 31
    • 0029879509 scopus 로고    scopus 로고
    • The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop
    • Dyer, J.M., McNew, J.A., and Goodman, J.M. (1996) The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop. J. Cell Biol. 133, 269-280
    • (1996) J. Cell Biol. , vol.133 , pp. 269-280
    • Dyer, J.M.1    McNew, J.A.2    Goodman, J.M.3
  • 32
    • 0035937842 scopus 로고    scopus 로고
    • Topogenesis of peroxisomal membrane protein requires a short, positively charged intervening-loop sequence and flanking hydrophobic segments, study using human membrane protein PMP34
    • Honsho, M. and Fujiki, Y. (2001) Topogenesis of peroxisomal membrane protein requires a short, positively charged intervening-loop sequence and flanking hydrophobic segments, study using human membrane protein PMP34. J. Biol. Chem. 276, 9375-9382.
    • (2001) J. Biol. Chem. , vol.276 , pp. 9375-9382
    • Honsho, M.1    Fujiki, Y.2
  • 33
    • 0026668020 scopus 로고
    • Signal peptide for peroxisomal targeting: Replacement of an essential histidine residue by certain amino acids converts the aminoterminal presequence of peroxisomal 3-ketoacyl-CoA thiolase to a mitochondrial signal peptide
    • Osumi, T., Tsukamoto, T., and Hata, S. (1992) Signal peptide for peroxisomal targeting: replacement of an essential histidine residue by certain amino acids converts the aminoterminal presequence of peroxisomal 3-ketoacyl-CoA thiolase to a mitochondrial signal peptide. Biochem. Biophys. Res. Commun. 186, 811-818
    • (1992) Biochem. Biophys. Res. Commun. , vol.186 , pp. 811-818
    • Osumi, T.1    Tsukamoto, T.2    Hata, S.3
  • 34
    • 0024319155 scopus 로고
    • Metabolic studies on citrate synthase mutants of yeast. A change in phenotype following transformation with an inactive enzyme
    • Kispal, G., Evans, C.T., Malloy, C., and Srere, P.A. (1989) Metabolic studies on citrate synthase mutants of yeast. A change in phenotype following transformation with an inactive enzyme. J. Biol. Chem. 264, 11204-11210
    • (1989) J. Biol. Chem. , vol.264 , pp. 11204-11210
    • Kispal, G.1    Evans, C.T.2    Malloy, C.3    Srere, P.A.4
  • 35
    • 0032491445 scopus 로고    scopus 로고
    • Interaction between citrate synthase and malate dehydrogenase. Substrate channeling of oxaloacetate
    • Morgunov, I. and Srere, P.A. (1998) Interaction between citrate synthase and malate dehydrogenase. Substrate channeling of oxaloacetate. J. Biol. Chem. 273, 29540-29544.
    • (1998) J. Biol. Chem. , vol.273 , pp. 29540-29544
    • Morgunov, I.1    Srere, P.A.2
  • 36
    • 0040777075 scopus 로고    scopus 로고
    • Model of a quinary structure between Krebs TCA cycle enzymes: A model for the metabolon
    • Velot, C., Mixon, M.B., Teige, M., and Srere, P.A. (1997) Model of a quinary structure between Krebs TCA cycle enzymes: a model for the metabolon. Biochemistry 36, 14271-14276.
    • (1997) Biochemistry , vol.36 , pp. 14271-14276
    • Velot, C.1    Mixon, M.B.2    Teige, M.3    Srere, P.A.4
  • 37
    • 0026459941 scopus 로고
    • Alternative topogenic signals in peroxisomal citrate synthase of Saccharomyces cerevisiae
    • Singh, K.K., Small, G.M., and Lewin, A.S. (1992) Alternative topogenic signals in peroxisomal citrate synthase of Saccharomyces cerevisiae. Mol. Cell. Biol. 12, 5593-5599
    • (1992) Mol. Cell. Biol. , vol.12 , pp. 5593-5599
    • Singh, K.K.1    Small, G.M.2    Lewin, A.S.3
  • 38
    • 0027507025 scopus 로고
    • Two independent peroxisomal targeting signals in catalase a of Saccharomyces cerevisiae
    • Kragler, F., Langeder, A., Raupachova, J., Binder, M., and Hartig, A. (1993) Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae. J. Cell Biol. 120, 665-673
    • (1993) J. Cell Biol. , vol.120 , pp. 665-673
    • Kragler, F.1    Langeder, A.2    Raupachova, J.3    Binder, M.4    Hartig, A.5
  • 39
    • 0028061714 scopus 로고
    • The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- And amino-terminal targeting signals
    • Waterham, H.R., Titorenko, V.I., Haima, P., Cregg, J.M., Harder, W., and Veenhuis, M. (1994) The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals. J. Cell Biol. 127, 737-749
    • (1994) J. Cell Biol. , vol.127 , pp. 737-749
    • Waterham, H.R.1    Titorenko, V.I.2    Haima, P.3    Cregg, J.M.4    Harder, W.5    Veenhuis, M.6
  • 40
    • 0028108279 scopus 로고
    • Characterization of the signal peptide at the amino terminus of the rat peroxisomal 3-ketoacyl-CoA thiolase precursor
    • Tsukamoto, T., Hata, S., Yokota, S., Miura, S., Fujiki, Y., Hijikata, M., Miyazawa, S., Hashimoto, T., and Osumi, T. (1994) Characterization of the signal peptide at the amino terminus of the rat peroxisomal 3-ketoacyl-CoA thiolase precursor. J. Biol. Chem. 269, 6001-6010
    • (1994) J. Biol. Chem. , vol.269 , pp. 6001-6010
    • Tsukamoto, T.1    Hata, S.2    Yokota, S.3    Miura, S.4    Fujiki, Y.5    Hijikata, M.6    Miyazawa, S.7    Hashimoto, T.8    Osumi, T.9


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