Expression and inhibitory activity analysis of a 25-kD Bowman-Birk protease inhibitor in rice

Chinese Science Bulletin

Volumn 51, Issue 1, 2006, Pages 54-62

  Chen, Jun ^a   Mao, Shengji ^a   Xie, Yang ^a   Cao, Zhongren ^a   Zhang, Yan ^a   Liu, Jing ^a   Chen, Zhangliang ^a,b   Qu, Lijia ^a,b   Gu, Hongya ^a,b  

^a PEKING UNIVERSITY   (China)

^b National Plant Gene Research Center   (China)

Author keywords

Bowman Birk inhibitor; Chymotrypsin inhibitory activity; Disulfide bond; Trypsin inhibitory activity

Indexed keywords

EID: 33748849594     PISSN: 10016538     EISSN: 18619541     Source Type: Journal    
DOI: 10.1007/s11434-005-0937-8     Document Type: Article

References (28)

1. Birk, Y., The Bowman-Birk inhibitor, Int. J. Pept. Protein Res., 1985, 25: 113-131.
2. Tashiro, M., Hashino, K., Shinozaki, M. et al., The complete amino acid sequence of rice bran trypsin inhibitor, J. Biochem., 1987, 102: 297-306.
3. Prakash, B., Selvaraj, S., Murthy, M.R.N. et al., Analysis of the amino acid sequences of plant Bowman-Birk inhibitors, J. Mol. Evol., 1996, 42: 560-569.
4. Werner, M. H., Wemmer, D. E., 3-Dimensional structure of soybean trypsin chymotrypsin Bowman-Birk inhibitor in solution, Biochemistry, 1992, 31: 999-1010.
5. Voss, R. H., Ermler, U., Essen, L. O. et al., Crystal structure of the bifunctional soybean Bowman-Birk inhibitor at 0.28 nm resolution - Structural peculiarities in a folded protein conformation, Eur. J. Biochem., 1996, 242: 122-131.
6. Li de la Sierra, I., Quillien, L., Flecker, P. et al., Dimeric crystal structure of a Bowman-Birk protcase inhibitor from pea seeds, J. Mol. Biol., 1999, 285: 1195-1207.
7. Murthy, H. M., Judge, K., DeLucas, L. et al., Crystal structure of Dengue virus NS3 protease in complex with a Bowman-Birk inhibitor: Implications for flaviviral polyprotcin processing and drug design, J. Mol. Biol., 2000, 301: 759-767.
8. Odani, S., Koide, T., Ono, T., Wheat germ trypsin inhibitors. Isolation and structural characterization of single-headed and double-headed inhibitors of the Bowman-Birk type, J. Biochem., 1986, 100: 975-983.
9. Tashiro, M., Asao, T., Hirata, C. et al., The complete amino acid sequence of a major trypsin inhibitor from seeds of foxtail millet (Setaria italica), J. Biochem. (Tokyo), 1990, 108: 669-672.
10. Nagasue, A., Fukamachi, H., Ikenaga, H. et al., The amino acid sequence of barley rootlet trypsin inhibitor, Agric. Biol. Chem., 1988, 52: 1505-1514.
11. Song, H. K., Kim, Y. S., Yang, J. K. et al., Crystal structure of a 16-kD double-headed Bowman-Birk trypsin inhibitor from barley seeds at 1.9Å resolution, J. Mol. Biol., 1999, 293: 1133-1144.
12. Bode, W., Huber, R., Natural protein proteinase inhibitors and their interactions with proteinases, Eur. J. Biochem., 1992, 204: 433 -451.
13. Domingo, G. J., Leatherbarrow, R. J., Freeman, N. et al., Synthesis of a mixture of cyclic peptides based on the Bowman-Birk reactive site loop to screen for serine protcase inhibitors, Tnt. J. Pept. Protein Res., 1995, 46:79-87.
14. Brauer, A. B., Kelly, G., McBride, J. D. et al., The Bowman-Birk inhibitor reactive site loop sequence represents an independent structural β-hairpinmotif, J. Mol. Biol., 2001, 306: 799-807.
15. McBride, J. D., Leatherbarrow, R. J., Synthetic peptide mimics of the Bowman-Birk inhibitor protein, Curr. Med. Chem., 2001, 8: 909-917.
16. Koiwa, H., Bressan, R. A., Hasegawa, P. M., Regulation of protease inhibitors and plant defense, Trends Plant Sci., 1997, 2: 379-384.
17. Qu, L. J., Chen, J., Liu, M. H. et al., Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice, Plant Physiol., 2003, 133: 560-570.
18. Chen, J., Liu, J., Guo, L. et al., Inducible expression pattern of rice Bowman-Birk inhibitor gone OsWIP1-2 and its protease inhibitory activity, Chinese Science Bulletin, 2004, 49: 895-899.
19. Li, N., Qu, L. J., Liu, Y. et al., The refolding, purification, and activity analysis of a rice Bowman-Birk inhibitor expressed in Escherichia coli, Protein Expr. Purif, 1999, 15: 99-104.
20. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 1976, 72: 248-254.
21. Hummel, B. C., A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin, Can. J. Biochem. Physiol., 1959, 37: 1394-1399.
22. Schwert, G. W., Takenaka, Y., A spectrophotometeric determination of trypsin and chymotrypsin, Biochem. Biophys. Acta, 1955, 16: 570-575.
23. Corey, D. R., Phillips, M. A., Cyclic peptides as proteases: A re-evaluation, Proc. Natl. Acad. Sci. USA, 1994, 91:4106-4109.
24. Wells, J. A., Fairbrother, W. J., Otlewski, J. et al., A reinvestigation of a synthetic peptide (TrPepz) designed to mimic trypsin, Proc. Natl. Acad. Sci. USA, 1994, 91: 4110-4114.
25. Chen, P. W., Chow, S. H., Chen, L. J., Nucleotide sequence of a cDNA (accession No. U76004) encoding rice Bowman-Birk proteinase inhibitor (PGR97-015), Plant Physiol., 1997, 113: 663.
26. Rakwal, R., Agrawal, G. K., Jwa, N. S., Characterization of a rice (Oryza sativa L.) Bowman-Birk proteinase inhibitor: tightly light regulated induction in response to cut, jasmonic acid, ethylene and proteinphosphatase 2Ainhibitors, Gene, 2001, 263: 189-198.
27. Laskowski, M. J., Kato, I., Protein inhibitors of proteinases, Ann. Rev. Biochem., 1980, 49: 593-626.
28. Philipp, S., Kim, Y.M., Dürr, I. et al., Mutational analysis of disulfide bonds in the trypsin-reactive subdomain of a Bowman-Birk-type inhibitor of trypsin and chymotrypsin - Cooperative versus autonomous refolding of subdomains, Eur. J. Biochem., 1998, 251: 854-862.