Expression of a part of the Potato virus A non-structural protein P3 in Escherichia coli for the purpose of antibody preparation and P3 immunodetection in plant material

Journal of Virological Methods

Volumn 137, Issue 2, 2006, Pages 229-235

  Novakova S ^a   Klaudiny, J ^b   Kollerova E ^a   Subr Z W ^a  

^a INSTITUTE OF VIROLOGY   (Slovakia)

^b INSTITUTE OF CHEMISTRY   (Slovakia)

Author keywords

Antibody preparation; Expression of proteins; Immunodetection; P3 protein; Transgenic plants

Indexed keywords

AMMONIUM SULFATE; ANTIBODY; PROTEIN P3; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; CLONE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; FEMALE; FILTRATION; IMMUNIZATION; IMMUNOBLOTTING; IMMUNODETECTION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POTATO VIRUS A; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INDUCTION; RABBIT; RNA VIRUS; SOLUBILITY; SUCROSE DENSITY GRADIENT CENTRIFUGATION; TEMPERATURE; TOBACCO; WILD TYPE;

ANTIBODIES, VIRAL; CELL FRACTIONATION; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FERMENTATION; IMMUNOBLOTTING; PLANT DISEASES; POTYVIRUS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TOBACCO; VIRAL NONSTRUCTURAL PROTEINS;

ESCHERICHIA COLI; NICOTIANA TABACUM; ORYCTOLAGUS CUNICULUS; POTATO VIRUS A;

EID: 33748687588     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.06.020     Document Type: Article

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