Expression, purification and anti-tumor activity of curcin

Acta Biochimica et Biophysica Sinica

Volumn 38, Issue 9, 2006, Pages 663-668

  Luo, Meng Jun ^a   Yang, Xin Yu ^a   Liu, Wei Xin ^b   Ying, X U ^a   Huang, Ping ^b   Yan, Fang ^a   Chen, Fang ^a  

^a SICHUAN UNIVERSITY   (China)

^b Chengdu Institute of Family Planning   (China)

Author keywords

Anti tumor activity; Curcin; Expression; Purification; Recombinant protein

Indexed keywords

ANTINEOPLASTIC AGENT; CURCIN PROTEIN, JATROPHA CURCAS; PLANT EXTRACT; RECOMBINANT PROTEIN; RIBOSOME INACTIVATING PROTEIN 1; VEGETABLE PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL PROLIFERATION; CELL SURVIVAL; CHEMISTRY; DOSE RESPONSE; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; JATROPHA; METABOLISM; NEOPLASM; PATHOLOGY; PLANT SEED; TUMOR CELL LINE;

ANIMALS; ANTINEOPLASTIC AGENTS, PHYTOGENIC; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; HUMANS; JATROPHA; NEOPLASMS; PLANT EXTRACTS; PLANT PROTEINS; RECOMBINANT PROTEINS; RIBOSOME INACTIVATING PROTEINS, TYPE 1; SEEDS;

ESCHERICHIA COLI; JATROPHA; JATROPHA CURCAS;

EID: 33748686346     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1111/j.1745-7270.2006.00208.x     Document Type: Article

References (23)

1. Girbes T, Ferreras JM, Arias FJ, Stirpe F. Description, distribution, activity and phylogenetic relationship of ribosome-inactivating proteins in plants, fungi and bacteria. Mini Rev Med Chem 2004, 4: 461-476
2. Nielsen K, Boston RS. Ribosome-inactivating proteins: A plant perspective. Annu Rev Plant Physiol Plant Mol Biol 2001, 52: 785-816
3. Park SW, Vepachedu R, Sharma N, Vivnco JM. Ribosome-inactivating proteins in plant biology. Planta 2004, 219: 1093-1096
4. Stirpe F. Ribosome-inactivating proteins. Toxicon 2004, 44: 371-383
5. Stirpe F, Pession-Brizzi A, Lorenzoni E, Strocchi P, Montanaro L, Sperti S. Studies on the proteins from the seeds of Croton tiglium and of Jatropha curcas. Toxic properties and inhibition of protein synthesis in vitro. Biochem J 1976, 156: 1-6
6. Zhang N, Wei Z, He J, Du L, Liang H. An efficient and economic method for preparation of high quality plant RNA. Prog Biochem Biophys 2004, 31: 947-950
7. Lin J, Chen Y, Xu Y, Yan F, Tang L, Chen F. Cloning and expression of curcin, a ribosome-inactivating protein from the seeds of Jatropha curcas. Acta Bot Sin 2003, 45: 858-863
8. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. New York: Cold Spring Harbor Laboratory Press 1989
9. Fang F, Zhou L. Modern Medical Experiment Protocol. Beijing: Chinese Academy Science & Pecking Union Medical College Press 1995
10. Du P. Medical Experimental Virus. Beijing: People's Military Medical Press 1985
11. Vepachedu R, Park SW, Sharma N, Vivanco JM. Bacterial expression and enzymatic activity analysis of ME1, a ribosome-inactivating protein from Mirabilis expansa. Protein Expr Purif 2005, 40: 142-151
12. Goto LS, Beltramini LM, de Moraes DI, Moreira RA, de Araujo AP. Abrus pulchellus type-2 RIP, pulchellin: Heterologous expression and refolding of the sugar-binding B chain. Protein Expr Purif 2003, 31: 12-18
13. Guo C, Li Z, Shi Y, Xu M, Wise JG, Trommer WE, Yuan J. Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin. Protein Expr Purif 2004, 37: 361-367
14. Rudolph R, Lilie H. In vitro folding of inclusion body proteins. FASEB J 1996, 10: 49-56
15. Ceciliani F, Caramori T, Ronchi S, Tedeschi G, Mortarino M, Galizzi A. Cloning, overexpression, and purification of Escherichia coli quinolinate synthetase. Protein Expr Purif 2000, 18: 64-70
16. Olsnes S, Fernandez-Puentes C, Carrasco L, Vazquez D. Ribosome inactivation by the toxic lectins abrin and ricin. Kinetics of the enzymic activity of the toxin A-chains. Eur J Biochem 1975, 60: 281-288
17. Zamboni M, Brigotti M, Rambelli F, Montanaro L, Sperti S. High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin. Biochem J 1989, 259: 639-643
18. Endo Y, Tsurugi K. RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes. J Biol Chem 1987, 262: 8128-8130
19. Brigotti M, Barbieri L, Valbonesi P, Stirpe F, Montanaro L, Sperti S. A rapid and sensitive method to measure the enzymatic activity of ribosome-inactivating proteins. Nucleic Acids Res 1998, 26: 4306-4307
20. Lin J, Yan F, Tang L, Chen F. Isolation, purification and functional investigation in the N-glycosidase activity of curcin from the seeds of Jatropha curcas. High Technology Letters 2002, 11: 36-40
21. Peumans WJ, Hao Q, Van Damme EJ. Ribosome-inactivating proteins from plants: More than RNA N-glycosidases? FASEB J 2001, 15: 1493-1506
22. Narayanan S, Surendranath K, Bora N, Surolia A, Karande AA. Ribosome inactivating proteins and apoptosis. FEES Lett 2005, 579: 1324-1331
23. Stirpe F, Barbieri L. Ribosome-inactivating proteins up to date. FEES Lett 1986, 195: 1-8