Cloning, expression and characterization of a thermostable exo-β-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii

Biotechnology Letters

Volumn 28, Issue 20, 2006, Pages 1655-1660

  Liu, Bo ^a   Li, Zhuo ^a   Hong, Ye ^a   Ni, Jinfeng ^a   Sheng, Duohong ^a   Shen, Yulong ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Denaturing agents; Exo D glucosaminidase; Pyrococcus horikoshii; Thermostability

Indexed keywords

DENATURING AGENTS; EXO-Β-D-GLUCOSAMINIDASE; GUANIDINE HYDROCHLORIDE; PYROCOCCUS HORIKOSHII;

CLONING; DIMERS; ENZYMES; ESCHERICHIA COLI; ETHANOL; THERMODYNAMIC STABILITY;

GENES;

BETA N ACETYLHEXOSAMINIDASE; DIMETHYL SULFOXIDE; DITHIOTHREITOL; DIVALENT CATION; DODECYL SULFATE SODIUM; EXO BETA D GLUCOSAMINIDASE; EXO-BETA-D-GLUCOSAMINIDASE; GUANIDINE; RECOMBINANT PROTEIN; UREA;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; DIMERIZATION; DRUG ANTAGONISM; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PH; PYROCOCCUS HORIKOSHII; TEMPERATURE;

CATIONS, DIVALENT; CLONING, MOLECULAR; DIMERIZATION; DIMETHYL SULFOXIDE; DITHIOTHREITOL; ENZYME STABILITY; ESCHERICHIA COLI; GUANIDINE; HEXOSAMINIDASES; HYDROGEN-ION CONCENTRATION; PYROCOCCUS HORIKOSHII; RECOMBINANT PROTEINS; SODIUM DODECYL SULFATE; TEMPERATURE; UREA;

ARCHAEA; ESCHERICHIA COLI; PYROCOCCUS HORIKOSHII;

EID: 33748582281     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9137-0     Document Type: Article

References (14)

1. Andronopoulou E, Vorgias C (2003) Purification and characterization of a new hyperthermostable, allosamidin-insensitive and denaturation-resistant chitinase from the hyperthermophilic archaeon Thermococcus chitonophagus. Extremophiles 7:43-53
2. Ando S, Ishida H, Kosugi Y, Ishikawa K (2002) Hyperthermostable endoglucanase from Pyrococcus horikoshii. Appl Environ Microbiol 68:430-433
3. Eom TK, Lee KM (2003) Characteristics of chitosanases from Aspergillus fumigatus KB-1. Arch Pharm Res 12:1036-1041
4. Egorova K, Antranikian G (2005) Industry relevance of thermophilic archaea. Curr Opin Microbiol 8:1-7
5. Jung WJ, Kuk JH, Kim KY, Jung KC, Park RD (2006) Purification and characterization of exo-β-D-glucosaminidase from Aspergillus fumigatus S-26. Protein Expr Purif 45:125-131
6. Kaper T, van Heusden HH, van Loo B, Vasella A, van der Oost J, de Vos WM (2002) Substrate specificity engineering of β-mannosidase and β-glucosidase from Pyrococcus horikoshii by exchange of unique active site residues. Biochemistry 41:4147-4155
7. Kawarabayasi Y, Sawada M, Horikawa H, Haikawa Y, Hino Y, Yamamoto S, Sekine M, Baba S, Kosugi H, Hosoyama A, Nagai Y, Sakai M, Ogura K, Otsuka R, Nakazawa H, Takamiya M, Ohfuku Y, Funahashi T, Tanaka T, Kudoh Y, Yamazaki J, Kushida N, Oguchi A, Aoki K, Kikuchi H (1998) Complete sequence and gene organization of the genome of a hyperthermophilic archaebacterium, Pyrococcus horikoshii OT3. DNA Res 5:55-76
8. Liu B, Ni JF, Shen YL (2006) Cloning, expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilic archaeon Pyrococcus horikoshii. Acta Microbiol Sinica (China) 46:255-258
9. Muzzarelli RA, Tanfani F, Scarpini G (1980) Chelating, filmforming, and coagulating ability of the chitosan-glucan complex from Aspergillus niger industrial wastes. Biotechnol Bioeng 22:885-896
10. Matsui I, Sakai Y, Matsui E, Kikuchi H, Kawarabayasi Y, Honda K (2000) Novel substrate specificity of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii. FEBS Lett 467:195-200
11. Nanjo F, Katsumi R, Sakai K (1990) Purification and characterization of an exo-β-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis. J Biol Chem 265:10088-10094
12. Shahidi F, Arachchi JKV, Jeon YJ (1999) Food applications of chitin and chitosans. Trends Food Sci Technol 10:37-51
13. Tanaka T, Fukui T, Fujiwara S, Atomi H, Imanaka T (2004) Concerted action of diacetylchitobiose deacetylase and exo-β-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Biol Chem 279:30021-30027
14. Vieille C, Zeikus GJ (2001) Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability. Microbiol Mol Biol Rev 65:1-43