Leishmania donovani: Effect of verapamil on in vitro susceptibility of promastigote and amastigote stages of Indian clinical isolates to sodium stibogluconate

Experimental Parasitology

Volumn 114, Issue 2, 2006, Pages 103-108

  Valiathan, Ranjini ^a   Dubey, M L ^a   Mahajan, R C ^a   Malla, Nancy ^a  

^a POSTGRADUATE INSTITUTE OF MEDICAL EDUCATION AND RESEARCH   (India)

Author keywords

acid phosphatase; AP; concentration at which 50 reduction in growth of organisms; Drug sensitivity; IC50; L. donovani; Leishmania donovani; p nitrophenyl phosphate; p NPP; SbV; sodium stibogluconate; V; verapamil; visceral leishmaniasis; VL

Indexed keywords

ACID PHOSPHATASE; CALCIUM CHANNEL BLOCKING AGENT; STIBOGLUCONATE SODIUM; VERAPAMIL;

AMASTIGOTE; ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; DRUG EFFECT; DRUG SENSITIVITY; IC 50; INDIA; LEISHMANIA DONOVANI; NONHUMAN; PRIORITY JOURNAL; PROMASTIGOTE; VISCERAL LEISHMANIASIS;

ANIMALS; ANTIMONY SODIUM GLUCONATE; ANTIPROTOZOAL AGENTS; CALCIUM CHANNEL BLOCKERS; DRUG RESISTANCE; DRUG SYNERGISM; INHIBITORY CONCENTRATION 50; LEISHMANIA DONOVANI; LIFE CYCLE STAGES; PARASITIC SENSITIVITY TESTS; VERAPAMIL;

LEISHMANIA DONOVANI;

EID: 33748538302     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2006.02.015     Document Type: Article

References (20)

1. Berman J.D., Edwards N., King M., and Grogl M. Biochemistry of pentostam resistant Leishmania. American Journal of Tropical Medicine and Hygiene 40 (1989) 159-164
2. Bhattacharyya A., Mukherjee M., and Duttagupta S. Studies on stibonate unresponsive isolates of Leishmania donovani. Journal of Biosciences 27 (2002) 503-508
3. Bora D. Epidemiology of visceral leishmaniasis in India. National Medical Journal of India 12 (1999) 62-68
4. Carrio M.C., Rieva C., Gallego M., Arboix M., and Portus M. Leishmania infantum stage specific activity of pentavalent antimony related with the assay conditions. Experimental Parasitology 95 (2000) 209-214
5. Chattopadhayay R., Kaur S., Ganguly N.K., and Mahajan R.C. Antigenic differences between axenic amastigotes and promastigotes of Leishmania donovani. Indian Journal Medical Research 104 (1996) 349-354
6. Dube A., Singh N., Sundar S., and Singh N. Refractoriness to the treatment of sodium stibogluconate in Indian kala-azar field isolates persist in in vitro and in vivo experimental models. Parasitology Research 96 4 (2005) 216-223
7. Fojo A., Akiyama S.I., Hottesman M.M., and Pastan I. Reduced drug accumulation in multiple drug resistant human KB carcinoma cell lines. Cancer Research 45 (1985) 3002-3007
8. Grogl M., Thomason T.N., and Franke E.D. Drug resistance in leishmaniasis: its implication in systemic chemotherapy of cutaneous and mucocutaneous disease. American Journal of Tropical Medicine and Hygiene 47 (1992) 117-126
9. Herwalt B.L. Leishmaniasis. Lancet 35 (1999) 1191-1199
10. Ibrahim M.E., Hag-Ali M., El-Hassan A.M., Theander T.G., and Kharazmi A. Leishmania resistant to Sodium stibogluconate: drug associated macrophage dependant killing. Parasitology Research 80 (1994) 569-574
11. Lira R., Sundar S., Makharia A., Kenney R., Gam A., Saraiva E., and Sakhs D. Evidence that the high incidence of treatment failure in Indian Kala azar is due to the emergence of antimony resistant strains of Leishmania donovani. The Journal of Infectious Diseases 180 (1999) 564-567
12. Martin S.K., Odula A.M.J., and Milhous W.K. Reversal of chloroquine resistance in P. falciparum by verapamil. Science 235 (1987) 899-901
13. Neal R.A., Bueren J.V., McCoy N.G., and Iwobi M. Reversal of drug resistance in T. cruzi and L. donovani by verapamil. Transactions of the Royal Society of Tropical Medicine and Hygiene 83 (1989) 197-198
14. Rao R.R., Mahajan R.C., and Ganguly N.K. Modified media for in-vitro cultivation of Leishmania donovani promastigotes. A comparative study. Bulletin PGI 18 (1984) 125-128
15. Sundar S. Drug resistance in Indian visceral leishmaniasis. Tropical Medicine and International Health 6 (2001) 849-854
16. Sundar S., Makharia A., and More D.K. Short-course oral miltefosine treatment for visceral leishmaniasis. Clinical Infectious Diseases 31 (2000) 1110-1113
17. Sundar S., More D.K., and Singh M.K. Failure of pentavalent antimony in visceral leishmaniasis in India report from the center of the Indian Epidemic. Clinical Infectious Diseases 31 (2000) 1104-1107
18. Thakur C.P., and Kumar M. Observations on the effect of Verapamil with Sodium stibogluconate in Kala Azar. Tropical and Geographical and Medicine 44 (1992) 15-18
19. Thakur C.P., Pandey A.K., Kumar M., Kumar P., Hassan S., Narain S., and Roy R.K. Do the diminishing efficacy and increasing toxicity of sodium stibogluconate in the treatment of VL in Bihar, India; justify its continued use as a first live drug? An observational study of 80 cases. Annals of Tropical Medicine and Parasitology 92 (1998) 561-569
20. WHO. Tropical disease research. 2005 .