Characterization of Human γ-glutamyl hydrolase in solution demonstrates that the enzyme is a non-dissociating homodimer

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1764, Issue 9, 2006, Pages 1479-1486

  Eisele, Leslie E ^a   Chave, Karen J ^a   Lehning, Andrew C ^a   Ryan, Thomas J ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

Antifolate; Folate metabolism; Glutamyl hydrolase; Homodimer; Polyglutamate

Indexed keywords

GAMMA GLUTAMYL HYDROLASE; HOMODIMER; HYBRID PROTEIN;

ANIMAL CELL; ARTICLE; CATALYSIS; CONCENTRATION (PARAMETERS); ENZYME ANALYSIS; IONIC STRENGTH; LIGHT SCATTERING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; RAT; ULTRACENTRIFUGATION; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; DIMERIZATION; GAMMA-GLUTAMYL HYDROLASE; HUMANS; LIGHT; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, QUATERNARY; RATS; SCATTERING, RADIATION; SEQUENCE ALIGNMENT; SOLUTIONS; ULTRACENTRIFUGATION;

MAMMALIA;

EID: 33748470343     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2006.06.008     Document Type: Article

References (16)

1. McGuire J.J., and Coward J.K. Pteroylpolyglutamates: biosynthesis, degradation, and function. In: Blakely R.L., and Benkovic S.L. (Eds). Folates and Pterins: Chemistry and Biochemistry of Folates vol. 1 (1984), John Wiley and Sons, Inc., New York 135-190
2. Yao R., Schneider E., Ryan T.J., and Galivan J. Human γ-glutamyl hydrolase: cloning and characterization of the enzyme expressed in vitro. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 10134-10138
3. Chave K.J., Galivan J., and Ryan T.J. Site-directed mutagenesis establishes cysteine 110 as essential for enzyme activity in human γ-glutamyl hydrolase. Biochem. J. 343 (1999) 551-555
4. Galivan J., Ryan T.J., Chave K.J., Rhee M., Yao R., and Yin D. Glutamyl hydrolase: pharmacological role and enzymatic characterization. Pharmacol. Ther. 85 (2000) 207-215
5. Chave K.J., Auger I.E., Galivan J., and Ryan T.J. Molecular modeling and site-directed mutagenesis define the catalytic motif in human γ-glutamyl hydrolase. J. Biol. Chem. 275 (2000) 40365-40370
6. Li H., Ryan T.J., Chave K.J., and Van Roey P. Three-dimensional structure of human γ-glutamyl hydrolase: a class I glutamine amidotransferase adapted for a complex substrate. J. Biol. Chem. 277 (2002) 24522-24529
7. Rhee M.S., Lindau-Shepard B., Chave K.J., Galivan J., and Ryan T.J. Characterization of human cellular γ-glutamyl hydrolase. Mol. Pharmacol. 53 (1998) 1040-1046
8. Yao R., Nimec Z., Ryan T.J., and Galivan J. Identification, cloning, and sequencing of a cDNA coding for rat γ-glutamyl hydrolase. J. Biol. Chem. 271 (1996) 8525-8528
9. Rhee M.S., Ryan T.J., and Galivan J.H. γ-Glutamyl hydrolase secreted from human tumor cell lines. Cell. Pharmacol. 2 (1995) 289-292
10. Schuck P. Size distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and Lamm equation modeling. Biophys. J. 78 (2000) 1606-1619
11. Philo J.S. A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions. Anal. Biochem. 279 (2000) 151-163
12. Schuck P. On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation. Anal. Biochem. 320 (2003) 104-124
13. O'Connor B.M., Rotundo R.F., Nimec Z., McGuire J.J., and Galivan J. Secretion of γ-glutamyl hydrolase in vitro. Cancer Res. 51 (1991) 3874-3881
14. Silink M., Reddel R., and Rowe P.B. Gamma-glutamyl hydrolase conjugase. Purification and properties of the bovine hepatic enzyme. J. Biol. Chem. 250 (1975) 5982-5994
15. Nooren I.M.A., and Thornton J.M. Diversity of protein-protein interactions. EMBO J. 22 (2003) 3486-3492
16. Orsomando G., Díaz de la Garza R., Green B.J., Peng M., Rea P.A., Ryan T.J., Gregory III J.F., and Hanson A.D. Plant γ-glutamyl hydrolases and folate polyglutamates: characterization, compartmentation, and co-occurrence in vacuoles. J. Biol. Chem. 280 (2005) 28877-28884