Microscopic Characterization of Follicular Structures in Letrozole-induced Polycystic Ovarian Syndrome in the Rat

Archives of Medical Research

Volumn 37, Issue 7, 2006, Pages 830-839

  Baravalle, Celina ^a   Salvetti, Natalia R ^a   Mira, Gustavo A ^a   Pezzone, Natalia ^a   Ortega, Hugo H ^a  

^a UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

Author keywords

Cadherins; Cellular proliferation; Intermediate filaments; Letrozole; Ovary; Polycystic ovarian syndrome; Rat

Indexed keywords

ESTRADIOL; FOLLITROPIN; LETROZOLE; LUTEINIZING HORMONE; PROGESTERONE; TESTOSTERONE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; ESTRADIOL BLOOD LEVEL; FEMALE; FOLLITROPIN BLOOD LEVEL; GRANULOSA CELL; HORMONE ACTION; IMMUNOHISTOCHEMISTRY; LUTEINIZING HORMONE BLOOD LEVEL; MICROSCOPY; NONHUMAN; OVARY FOLLICLE; OVARY POLYCYSTIC DISEASE; PROGESTERONE BLOOD LEVEL; RADIOIMMUNOASSAY; RAT;

ANIMALS; CADHERINS; DESMIN; FEMALE; HORMONES; IMMUNOHISTOCHEMISTRY; KERATINS; NITRILES; OVARIAN FOLLICLE; POLYCYSTIC OVARY SYNDROME; PROLIFERATING CELL NUCLEAR ANTIGEN; RATS; RATS, WISTAR; TRIAZOLES; VIMENTIN;

EID: 33748466507     PISSN: 01884409     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.arcmed.2006.04.006     Document Type: Article

References (84)

1. Xita N., Georgiou I., and Tsatsoulis A. The genetic basis of polycystic ovary syndrome. Eur J Endocrinol 147 (2002) 717-725
2. Mahesh V.B., Mills T.M., Bagnell C.A., and Conway B.A. Animal models for study of polycystic ovaries and ovarian atresia. Adv Exp Med Biol 219 (1987) 237-257
3. Mahajan D.K. Polycystic ovarian disease: animals models. Endocrinol Metab Clin 17 (1988) 705-732
4. Kalra S.P., and Kalra P.S. Neural regulation of luteinizing hormone secretion in the rat. Endocr Rev 4 (1983) 311-351
5. Jones H.M., Vernon M.W., and Rush M.E. Systematic studies invalidate the neonatally androgenized rat as a model for polycystic ovary disease. Biol Reprod 36 (1987) 1253-1265
6. Critchlow B.V. The role of light in the neuroendocrine system. In: Nalbandov A.V. (Ed). Advances in Neuroendocrinology (1989), University of Illinois Press, Urbana, IL
7. Salvetti N.R., Gimeno J.E., Canal A.M., Lorente J., and Ortega H.H. Histochemical study of the extracellular matrix components in the follicular wall of induced polycystic ovaries. Braz J Morphol Soc 20 (2003) 3-10
8. Salvetti N., Canal A.M., Gimeno E.J., and Ortega H.H. Polycystic ovarian sindrome: temporal characterization of the induction and reversion process in an experimental model. Braz J Vet Res Anim Sci 41 (2004) 389-395
9. Salvetti N.R., Gimeno E.J., Lorente J.A., and Ortega H.H. Expression of cytoskeletal proteins in the follicular wall of induced ovarian cysts. Cells Tissues Organs 178 (2004) 117-125
10. Singh K.B. Induction of polycystic ovarian disease in rats by continuous light. Am J Obstet Gynecol 104 (1969) 1079-1083
11. Kafali H., Iriadam M., Ozardali I., and Demir N. Letrozole-induced polycystic ovaries in the rat: a new model for cystic ovarian disease. Arch Med Res 35 (2004) 103-108
12. Barberi R.L. Polycystic ovarian disease. Annu Rev Med 42 (1991) 199-204
13. Agarwal S.K., and Shankar U. Clinical response, related progesterone profile and subsequent fertility after gonadotropin releasing hormone administration in crossbred cows with ovarian cyst. Ind Vet J 75 (1998) 127-130
14. Wiltbank M.C., Gumen A., and Sartori R. Physiological classification of anovulatory conditions in cattle. Theriogenology 57 (2003) 21-52
15. Hoo P., and Shaw R. The patient with polycystic ovary syndrome. Practitioner 249 (2005) 555-557
16. Raja A., Hashmi S.N., Sultana N., and Rashid H. Presentation of polycystic ovary syndrome and its management with clomiphene alone and in combination with metformin. J Ayub Med Coll Abbottabad 17 (2005) 50-53
17. Lopez-Díaz M.C., and Bosu W.T.K. A review and an update of cystic ovarian degeneration in ruminants. Theriogenology 37 (1992) 1163-1183
18. Hamilton S.A., Garverick H.A., Keisler D.H., Xu Z.Z., Loos K., Youngquist R.S., et al. Characterization of ovarian follicular cysts and associated endocrine profiles in dairy cows. Biol Reprod 53 (1995) 890-898
19. Voss A.K., and Fortune J.E. Estradiol-17b has a biphasic effect on oxytocin secretion by bovine granulosa cells. Biol Reprod 48 (1993) 1404-1409
20. Isobe N., Nakao T., and Yoshimura Y. Immunohistochemical localization of 3 B hydroxysteroid dehydrogenase in the granulosa and theca interna layers of bovine cystic follicles. J Reprod Dev 49 (2003) 227-233
21. Perrone M.S., Peter A.T., and Asem E.K. Fibronectins: role in reproduction and future applications. Assist Reprod Technol Androl 7 (1995) 103-125
22. Peter A.T., Perrone M.S., and Asem E.K. Bovine ovarian follicular fluid vitronectin content is influenced by follicle size. Theriogenology 43 (1995) 1239-1247
23. Santini D., Ceccarelli C., Mazzoleni G., Pasquinelli G., Jasonni V.M., and Martinelli G.N. Demonstration of cytokeratin intermediate filaments in oocytes of the developing and adult human ovary. Histochemistry 99 (1993) 311-319
24. Selstam G., Nilsson I., and Mattson M.O. Changes in the ovarian intermediate filament desmin during the luteal phase of the adult pseudopregnant rat. Acta Physiol Scand 147 (1993) 123-129
25. van Wely M., Bayram N., van der Veen F., and Bossuy P.M. Predictors for treatment failure after laparoscopic electrocautery of the ovaries in women with clomiphene citrate resistant polycystic ovary syndrome. Obstet Gynecol Surv 60 (2005) 591-593
26. Khan-Dawood F.S., Dawood M.Y., and Tabibzadeh S. Immunohistochemical analysis of the microanatomy of primate ovary. Biol Reprod 54 (1996) 734-742
27. Khan-Dawood F.S., Yang J., and Dawood Y. Immunohistochemical localization and expression of α-actin in the baboon (Papio anubis) corpus luteum. J Histochem Cytochem 45 (1997) 71-77
28. Pan J., and Auersperg N. Spatiotemporal changes in cytokeratin expression in the neonatal rat ovary. Biochem Cell Biol 76 (1998) 27-35
29. Löffler S., Horn L.C., Weber W., and Spanel-Borowski K. The transient disappearance of cytokeratin in human fetal and adult ovaries. Anat Embryol 201 (2000) 207-215
30. Marettova E., and Maretta M. Demonstration of intermediate filaments in sheep ovary. Acta Histochem 104 (2002) 431-434
31. Farookhi R., and Blaschuk O.W. Cadherins and ovarian follicular development. In: Gibori G. (Ed). Signalling Mechanisms and Gene Expression in the Ovary. Serono Symposia (1991), Springer Verlag, New York 254-260
32. Rowlands T., Symonds J.M., Farookhi R., and Blaschuk O.W. Cadherins: crucial regulators of structure and function in reproductive tissues. Rev Reprod 5 (2000) 53-61
33. Kirkup K.M., Mallin A.M., and Bagnell C.A. Inhibition of pig granulosa cell adhesion and growth in vitro by immunoneutralization of epithelial cadherin. J Reprod Fertil 120 (2000) 275-281
34. Ryan P.L., Valentine A.F., and Bagnell C.A. Expression of epithelial cadherin in the developing and adult pig ovary. Biol Reprod 55 (1996) 1091-1097
35. Farookhi R., and Desjardins J. Luteinizing hormone receptor induction in dispersed granulosa cells requires estrogen. Mol Cell Endocrinol 47 (1986) 13-24
36. Peluso J.J., Pappalardo A., and Trolice M.P. N-cadherin-mediated cell contact inhibits granulosa cell apoptosis in a progesterone-independent manner. Endocrinology 137 (1996) 1196-1203
37. Makrigiannakis A., Coukos G., Christofidou-Slolomidou M., Gour B.J., Radice G.L., Blaschuk O., et al. N-cadherin-mediated human granulosa cell adhesion prevents apoptosis: a role in follicular atresia and luteolysis?. Am J Pathol 154 (1999) 1391-1406
38. Guadagno T.M., Ohtsubo M., Roberts J.M., and Assoian R.K. A link between cyclin A expression and adhesion-dependent cell cycle progression. Science 262 (1993) 1572-1575
39. Ruoslahti E., and Reed J.C. Anchorage dependence, integrins and apoptosis. Cell 77 (1994) 477-478
40. Isobe N., and Yoshimura Y. Localization of apoptotic cells in the cystic ovarian follicles of cows: a DNA-end labeling histochemical study. Theriogenology 53 (2000) 897-904
41. Isobe N., and Yoshimura Y. Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows. Theriogenology 54 (2000) 1159-1169
42. National Research Council. Guide for the Care and Use of Laboratory Animals (1996), National Academy Press, Washington, DC
43. Ortega H.H., Lorente J.A., Mira G.A., Baravalle C., and Salvetti N.R. Constant light exposure causes dissociation in gonadotrophins secretion and inhibits partially neuroendocrine differentiation of Leydig cells in adult rats. Reprod Domest Anim 39 (2004) 417-423
44. Ranefall P., Wester K., and Bengtsson E. Automatic quantification of immunohistochemically stained cell nuclei using unsupervised image analysis. Anal Cell Pathol 16 (1998) 29-43
45. Gimeno E.J., Massone A.R., and Portiansky E.L. Preembedding epitope retrieval: an ultrasound-based method for unmasking desmin in tissue blocks. Appl Immunohistochem Mol Methods 6 (1998) 35-41
46. Ellis C.M., Dyson M.J., Stephenson T.J., and Maltby E.L. HER2 amplification status in breast cancer: a comparison between immunohistochemical staining and fluorescence in situ hybridisation using manual and automated quantitative image analysis scoring techniques. J Clin Pathol 58 (2005) 710-714
47. Witkiewicz A.K., Varambally S., Shen R., Mehra R., Sabel M.S., Ghosh D., et al. Alpha-methylacyl-CoA racemase protein expression is associated with the degree of differentiation in breast cancer using quantitative image analysis. Cancer Epidemiol Biomarkers 14 (2005) 1418-1423
48. Chamson-Reig A., Bianchi M.S., Rey-Roldan E., Sorianello E., Libertun C., and Lux-Lantos V. Development of an experimental ovarian tumor over a year in the rat. Life Sci 65 (1999) 1275-1285
49. Bianchi M.S., Catalano P.N., Bonaventura M.M., Silveyra P., Bettler B., Libertun C., et al. Effect of androgens on sexual differentiation of pituitary gamma-aminobutyricacid receptor subunit GABA(B) expression. Neuroendocrinology 80 (2004) 129-142
50. Abbott D.H., Dumesic D.A., and Franks S. Developmental origin of polycystic ovary syndrome-a hypothesis. J Endocrinol 17 (2002) 41-45
51. Ambrose D.J., Schmitt E.J., Lopes F.L., Mattos R.C., and Thatcher W.W. Ovarian and endocrine responses associated with the treatment of cystic ovarian follicles in dairy cows with gonadotropin releasing hormone and prostaglandin F2alpha, with or without exogenous progesterone. Can Vet J 45 (2004) 931-937
52. Ashmawy A.A., Vogt D.W., Youngquist R.S., and Garverick H.A. Heritability of liability to cystic ovary development in Holstein cattle. J Hered 81 (1990) 165-166
53. Garverick H.A. Ovarian follicular cysts in dairy cows. J Dairy Sci 80 (1997) 995-1004
54. Peter A.T. An update on cystic ovarian degeneration in cattle. Reprod Domest Anim 39 (2004) 1-7
55. Silvia W.J., Hatler T.B., Nugent A.M., and Laranja da Fonseca L.F. Ovarian follicular cysts in dairy cows: an abnormality in folliculogenesis. Domest Anim Endocrinol 23 (2002) 167-177
56. Legro R.S., Urbanek M., Kunselman A.R., Leiby B.E., and Dunaif A. Self-selected women with polycystic ovary syndrome are reproductively and metabolically abnormal and undertreated. Fertil Steril 78 (2002) 51-57
57. Kumar A., Woods K.S., Bartolucci A.A., and Azziz R. Prevalence of adrenal androgen excess in patients with the polycystic ovary syndrome (PCOS). Clin Endocrinol 62 (2005) 644-649
58. Ben-Ze'ev A., and Amsterdam A. In vitro regulation of granulosa cell differentiation. J Biol Chem 262 (1987) 5366-5376
59. Anderson E., and Lee G.Y. The polycystic ovarian (PCOD) condition: apoptosis and epithelization of the ovarian antral follicles are aspects of cystogenesis in the dehydroepiandrosterone (DHEA)-treated rat model. Tissue Cell 29 (1997) 171-189
60. Irving-Rodgers H.F., Harland M.L., and Rodgers R.J. A novel basal lamina matrix of the stratified epithelium of the ovarian follicle. Matrix Biol 23 (2004) 207-217
61. Raymond W.A., and Leong A.S. Vimentin, a new prognostic parameter in breast carcinoma. J Pathol 158 (1989) 107-114
62. Domagala W., Lasota J., Duckowicz A., Markiewski M., Striker G., Weber K., et al. Vimentin expression appears to be associated with poor prognosis in node-negative ductal NOS breast carcinomas. Am J Pathol 137 (1990) 1299-1304
63. van den Hurk R., Dijkstra G., Van Mil F.N., Hulshof S.C., and Van den Ingh T.S. Distribution of the intermediate filament proteins vimentin, keratin, and desmin in the bovine ovary. Mol Reprod Dev 41 (1995) 459-467
64. Ghadially F.N., Janzen H.K., and Mehta P.N. Synovial membrane in experimental lipoarthrosis. Arch Pathol 89 (1970) 291-301
65. Pieraggi M.T., Julian M., and Bouissou H. Fibroblast changes in cutaneous ageing. Virchows Arch A Pathol Anat Histopathol 402 (1984) 275-287
66. Paranko J., and Virtanen I. Epithelial and mesenchymal cell differentiation in the fetal rat genital ducts: changes in the expression of cytokeratin and vimentin type of intermediate filaments and desmosomal plaque proteins. Dev Biol 117 (1986) 135-145
67. Frojdman K., Paranko J., Virtanen I., and Pelliniemi L.J. Intermediate filaments and epithelial differentiation of male rat embryonic gonad. Differentiation 50 (1992) 113-123
68. Ylagan L.R., Middleton W.D., and Dehner L.P. Fine-needle aspiration cytology of recurrent granulosa cell tumor: case report with differential diagnosis and immunocytochemistry. Diagn Cytopathol 27 (2002) 38-41
69. Ozbey C., Erdogan G., Pestereli H.E., Simsek T., and Karaveli S. Serous papillary adenocarcinoma and adult granulosa cell tumor in the same ovary. An unusual case. APMIS 113 (2005) 713-715
70. Connell N.D., and Rheinwald J.G. Regulation of the cytoskeleton in mesothelial cells: reversible loss of keratin and increase in vimentin during rapid growth in culture. Cell 34 (1983) 245-253
71. LaRocca P.J., and Rheinwald J.G. Coexpression of simple epithelial keratins and vimentin by human mesothelium and mesothelioma in vivo and in culture. Cancer Res 44 (1984) 2991-2999
72. Merchant-Larios H. Origin of the somatic cells in the rat gonad: an autoradiographic approach. Ann Biol Animale Biochim Biophys 19 (1979) 1219-1229
73. Czernobilsky B., Moll R., Levy R., and Franke W.W. Co-expression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of the human ovary. Eur J Cell Biol 37 (1985) 175-190
74. Benjamin E., Law S., and Bobrow L.G. Intermediate filaments cytokeratin and vimentin in ovarian sex cord-stromal tumours with correlative studies in adult and fetal ovaries. J Pathol 152 (1987) 253-263
75. Gall L., De Smedt V., and Rufini S. Co-expression of cytokeratins and vimentin in sheep cumulus-oocyte complexes. Alteration of intermediate filament distribution by acrylamide. Dev Growth Differ 34 (1992) 579-587
76. Lane E.B., and Alexander C.M. Use of keratin antibodies in tumor diagnosis. Semin Cancer Biol 1 (1990) 165-179
77. Erickson G.F., Magoffin D.A., Garzo V.G., Cheung A.P., and Chang R.J. Granulosa cells of polycystic ovaries: are they normal or abnormal?. Hum Reprod 7 (1992) 293-299
78. Blaschuk O.W., and Farookhi R. Estradiol stimulates cadherin expression in rat granulosa cells. Dev Biol 136 (1989) 564-567
79. Harandian F., and Farookhi R. Contact-dependent cell interactions determine hormone responsiveness and desensitization in rat granulose cells. Endocrinology 39 (1998) 1700-1707
80. Machell N.H., Blaschuk O.W., and Farookhi R. Developmental expression and distribution of N- and E-cadherin in the rat ovary. Biol Reprod 63 (2000) 797-804
81. McNeill H., Ozawa M., Kemler R., and Nelson W.J. Novel function of the cell adhesion molecule uvomorulin as an inducer of cell surface polarity. Cell 62 (1990) 309-316
82. Jolly P.D., Tisdall D.J., Heath D.A., Lun S., and McNatty K.P. Apoptosis in bovine granulosa cells in relation to steroid synthesis, cyclic adenosine 3′, 5′-monophosphate response to follicle-stimulating hormone and luteinizing hormone, and follicular atresia. Biol Reprod 51 (1994) 934-944
83. Jolly P.D., Smith P.R., Heath D.A., Hudson N.L., Lun S., Still L.A., et al. Morphological evidence of apoptosis and the prevalence of apoptotic versus mitotic cells in the membrana granulosa of ovarian follicles during spontaneous and induced atresia in ewes. Biol Reprod 56 (1997) 837-846
84. Shao R., Markstrom E., Friberg P.A., Johansson M., and Billig H. Expression of progesterone receptor (PR) A and B isoforms in mouse granulosa cells: stage-dependent PR-mediated regulation of apoptosis and cell proliferation. Biol Reprod 68 (2003) 914-921