Preembedding epitope retrieval: An ultrasound-based method for unmasking desmin in tissue blocks

Applied Immunohistochemistry

Volumn 6, Issue 1, 1998, Pages 35-41

  Gimeno, Eduardo Juan ^a,b   Massone, Adriana Raquel ^a   Portiansky, Enrique Leo ^a  

^a UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^b FACULTAD DE CIENCIAS VETERINARIAS   (Argentina)

Author keywords

Desmin; Epitope retrieval; Tissue blocks; Ultrasound

Indexed keywords

DESMIN; EPITOPE;

ANIMAL TISSUE; ANTIGEN RECOGNITION; ANTIGEN RETRIEVAL; ARTICLE; EMBEDDING; NONHUMAN; PRIORITY JOURNAL; SKELETAL MUSCLE; STAINING; SWINE; ULTRASOUND;

EID: 0031943005     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-199803000-00007     Document Type: Article

References (25)

1. Puchtler H, Meloan SN. On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions. Histochemistry 1985;82:201-4.
2. Leong ASY, Gilham PN. The effects of progressive formaldehyde fixation on the preservation of tissue antigens. Pathology 1989;21: 266-8.
3. Fox CH, Johnson FB, Whiting J, Roller PP. Formaldehyde fixation. J Histochem Cytochem 1985;33:845-53.
4. Masson JT, O'Leary TJ. Effects of formaldehyde fixation on protein structure: a colorimetric and infrared spectroscopic investigation. J Histochem Cytochem 1991;39:225-9.
5. Hopwood D. Cell and tissue fixation. Histochem J 1985;17:389-442.
6. Boon EN, Gerrits PO, Moorlag HE, Nieuwenhuis P, Kok LP. Formaldehyde fixation and microwave irradiation. Histochem J 1988;20:313-22.
7. Dürgun-Yücel B, Hopwood D, Yücel AH. The effects of mercaptoethanol-formaldehyde on tissue fixation and protein retention. Histochem J 1996;28:375-383.
8. Portiansky EL, Massone AR, Gimeno EJ. Kinetics of epitope retrieval technique for unmasking cytokeratins in bovine prostatic tissue after different formaldehyde fixation times. Appl Immunohistochem 1997;5:194-201.
9. Huang SN, Minassian H, More JD. Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab Invest 1976;35:383.
10. Battifora H, Kopinski M. The influence of protease digestion and duration of fixation on the immunostaining of keratins. J Histochem Cytochem 1986;34:1095-1100.
11. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39:741-8.
12. Cattoretti G, Suurmeijer AJH. Antigen unmasking on formalinfixed paraffin-embedded tissues using microwaves: a review. Adv Anal Pathol 1995;2:2-9.
13. Taylor CR, Shi SR, Cote RJ. Review: antigen retrieval for immunohistochemistry. Appl Immunohistochem 1996;4:144-66.
14. Portiansky EL, Gimeno EJ. A new epitope retrieval method for the detection of structural cytokeratins in the bovine prostatic tissue. Appl Immunohistochem 1996;4:208-14.
15. Battifora H. HIER here! [editorial]. Appl Immunohistochem 1996; 4:143.
16. Cattoretti G, Pileri S, Parravicini C, et al. Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J Pathol 1993; 171:83-98.
17. Bankfalvi A, Navabi H, Bier B, Böcker W, Jasani B, Schmid KW. Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry. J Pathol 1994;174:223-8.
18. Norton AJ, Jordan S, Yeomans P. Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J Pathol 1994; 173:371-9.
19. Wells WA, Rainer RO, Memoli VA. Equipment, standardization, and application of image processing. Am J Clin Pathol 1993;99: 48-56.
20. Barbareschi M, Weidner N, Gasparini G, et al. Microvessel density quantification in breast carcinomas. Appl Immunohistochem 1995; 3:75-84.
21. Zar JH. Multiple comparisons. In Zar JH, ed. Biostatistical analysis. Englewood Cliffs, NJ: Prentice Hall, 1984:185-205.
22. Evers P, Uylings HM. Effects of microwave pretreatment of immunocytochemistry staining of vibratome sections and tissue blocks of human cerebral cortex stored in formaldehyde for long periods. J Neurosci Methods 1994;55:163-72.
23. Horder MM, Barnett SB, Edwards MJ. Diagnostic ultrasound un veterinary practice: how safe is it?. Austr Vet J 1996;73:10-15.
24. Morgan JM, Navabi H, Schmidt KW, Jasani B. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J Pathol 1994;174:301-7.
25. Brynes RK, McCourty A, Tamayo R, Jenkins K, Battifora H. Demonstration of cyclin D1 (Bcl-1) in mantle cell lymphoma. Enhance staining using heat and ultrasound epitope retrieval. Appl Immunohistochem 1997;5:45-8.