Novel nontoxic mitochondrial probe for confocal fluorescence microscopy

Journal of Biomedical Optics

Volumn 11, Issue 3, 2006, Pages

  Versari, Silvia ^a   Villa, Anna Maria ^b   Villa, Alessandro ^a   Doglia, Silvia Maria ^b   Pagani, Giorgio A ^b   Bradamante, Silvia ^a  

^a CNR   (Italy)

^b UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Chromophore; Confocal fluorescence microscopy; Donor acceptor system; Fluorescence; Mitochondrial stain

Indexed keywords

CELL CULTURE; CHROMOPHORES; DNA; DYES; FLUORESCENCE; PROBES; TOXICITY;

CONFOCAL FLUORESCENCE MICROSCOPY; DONOR ACCEPTOR SYSTEM; MITOCHONDRIAL STAIN;

OPTICAL MICROSCOPY;

MAMMALIA;

1 METHYL 2,5 BIS(2 (1 METHYLPYRIDINIUM 4 YL)ETHENYL)PYRROLE; 1-METHYL-2,5-BIS(2-(1-METHYLPYRIDINIUM-4-YL)ETHENYL)PYRROLE; DIAGNOSTIC AGENT; FLUORESCENT DYE; MITOCHONDRIAL DNA; PYRIDINIUM DERIVATIVE; PYRROLE DERIVATIVE;

ANIMAL; ARTICLE; CONFOCAL MICROSCOPY; DRUG EFFECT; FLUORESCENCE MICROSCOPY; HAPLORHINI; HUMAN; METABOLISM; METHODOLOGY; MITOCHONDRION; ULTRASTRUCTURE;

ANIMALS; DNA, MITOCHONDRIAL; FLUORESCENT DYES; HAPLORHINI; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; PYRIDINIUM COMPOUNDS; PYRROLES;

EID: 33748459469     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.220617     Document Type: Article

References (12)

1. A. Abbotto, L. Beverina, R. Bozio, A. Facchetti, C. Ferrante, G. A. Pagani, D. Pedron, and R. Signorini, "Novel heterocycle-based twophoton absorbing dyes", Org. Lett. 4, 1495-1498 (2002).
2. G. Pagani, A. Abbotto, and R. Bozio, "Heteroaromatic compounds having two-photon absorption activity", PCT Inl. Appl. (2001).
3. A. Abbotto, G. Baldini, L. Beverina, G. Chirico, M. Collini, L. D'Alfonso, A. Diaspro, R. Magrassi, L. Nardo, and G. A. Pagani, "Dimethyl-pepep: a DNA probe in two-photon excitation cellular imaging", Biophys. Chem. 114, 35-41 (2005). (Pubitemid 40403528)
4. S. M. Doglia, L. Bianchi, R. Colombo, N. Allam, H. Morjani, M. Manfait, and A. M. Villa, "Confocal fluorescence microscopy of living cells", Proc. SPIE 1922, 126-134 (1993).
5. A. M. Villa and S. M. Doglia, "Mitochondria in tumor cells studied by laser scanning confocal microscopy", J. Biomed. Opt. 9(2), 385-394 (2004).
6. J. Frederic, "Cytological research on the normal chondriome or the chondriome subjected to experimentation in living cells cultivated in vitro", Arch. Biol. (Liege) 69, 167-349 (1958).
7. V. P. Skulachev, "Mitochondrial filaments and clusters as intracellular powertransmitting cables", Trends Biochem. Sci. 26, 23-29 (2001). (Pubitemid 32124706)
8. M. Dellinger and M. Geze, "Detection of mitochondrial DNA in living animal cells with fluorescence microscopy", J. Microsc. 204, 196-202 (2001). (Pubitemid 34075117)
9. M. R. Duchen, "Mitochondria in health and disease: perspectives on a new mitochondrial biology", Mol. Aspects Med. 25, 365-451 (2004). (Pubitemid 39070333)
10. L. V. Johnson, M. L. Walsh, and L. B. Chen, "Localization of mitochondria in living cells with rhodamine 123", Proc. Natl. Acad. Sci. U. S. A. 77, 990-994 (1980). (Pubitemid 10105043)
11. L. V. Johnson, M. L. Walsh, B. J. Bockus, and L. B. Chen, "Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy", J. Cell Biol. 88, 526-535 (1981). (Pubitemid 11097944)
12. M. Coppey-Moisan, A. Brunet, R. Morais, and J. Coppey, "Dynamical change of mitochondrial DNA induced in the living cell by perturbing the electrochemical gradient", Biophys. J. 71, 2319-2328 (1996). (Pubitemid 26367698)