Cloning, embryonic expression, and functional characterization of two novel connexins from Xenopus laevis

Biochemical and Biophysical Research Communications

Volumn 349, Issue 2, 2006, Pages 855-862

  de Boer, Teun P ^a   Kok, Bart ^a   Roël, Giulietta ^b   van Veen, Toon A B ^a   Destrée, Olivier H J ^b   Rook, Martin B ^a   Vos, Marc A ^a   de Bakker, Jacques M T ^a   van der Heyden, Marcel A G ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b HUBRECHT INSTITUTE   (Netherlands)

Author keywords

Cement gland; Connexin; Electrophysiology; Endoderm; Expression; Gap junction; Liver anlage; Mesoderm; Organizer; Phylogeny; Pronephros; Xenopus

Indexed keywords

GAP JUNCTION PROTEIN; MESSENGER RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL COMMUNICATION; CELL STRAIN COS7; CELL STRAIN HEK293; CONTROLLED STUDY; ELECTROPHYSIOLOGY; EMBRYO; EMBRYO DEVELOPMENT; ENDODERM; FLUORESCENCE MICROSCOPY; GAP JUNCTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; LIVER; MESODERM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OSMOREGULATION; PHYLOGENY; PRIMORDIUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; WESTERN BLOTTING; XENOPUS LAEVIS;

AMINO ACID SEQUENCE; ANIMALS; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; CONNEXINS; COS CELLS; GAP JUNCTIONS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HUMANS; MESODERM; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; XENOPUS LAEVIS; XENOPUS PROTEINS;

VERTEBRATA; XENOPUS LAEVIS;

EID: 33748436044     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.08.121     Document Type: Article

References (25)

1. Phelan P. Innexins: members of an evolutionarily conserved family of gap-junction proteins. Biochim. Biophys. Acta 1711 (2005) 225-245
2. Sáez J.C., Berthoud V.M., Brañes M.C., Martínez A.D., and Beyer E.C. Plasma membrane channels formed by connexins: their regulation and functions. Physiol. Rev. 83 (2003) 1359-1400
3. Panchin Y.V. Evolution of gap junction proteins-the pannexin alternative. J. Exp. Biol. 208 (2005) 1415-1419
4. Sosinsky G.E., and Nicholson B.J. Structural organization of gap junction channels. Biochim. Biophys. Acta 1711 (2005) 99-125
5. Sáez J.C., Retamal M.A., Basilio D., Bukauskas F.F., and Bennett M.V. Connexin-based gap junction hemichannels: gating mechanisms. Biochim. Biophys. Acta 1711 (2005) 215-224
6. de Boer T.P., and van der Heyden M.A.G. Xenopus connexins: how frogs bridge the gap. Differentiation 73 (2005) 330-340
7. Cruciani V., and Mikalsen S.-O. The vertebrate connexin family. Cell. Mol. Life Sci. 63 (2006) 1125-1140
8. Kumar S., Tamura K., and Nei M. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformat. 5 (2004) 150-163
9. Nieuwkoop P.D., and Faber J. Normal table of Xenopus laevis (Daudin). second ed. (1967), North Holland, Amsterdam, The Netherlands
10. Niehrs C., Steinbeiser H., and de Robertis E.M. Mesodermal patterning by a gradient of the vertebrate homeobox gene goosecoid. Science 263 (1994) 817-820
11. Harland R.M. In situ hybridization: an improved whole-mount method for Xenopus embryos. Methods Cell Biol. 36 (1991) 685-695
12. de Boer T.P., Kok B., Neuteboom K.I.E., Spieker N., de Graaf J., Destrée O.H.J., Rook M.B., van Veen T.A.B., Jongsma H.J., Vos M.A., de Bakker J.M.T., and van der Heyden M.A.G. Cloning and functional characterization of a novel connexin expressed in somites of Xenopus laevis. Dev. Dyn. 233 (2005) 864-871
13. Willecke K., Eiberger J., Degen J., Eckardt D., Romualdi A., Güldenagel M., Deutsch U., and Söhl G. Structural and functional diversity of connexin genes in the mouse and human genomes. Biol. Chem. 383 (2002) 725-737
14. Söhl G., and Willecke K. An update on connexin genes and their nomenclature in mouse and man. Cell Commun. Adhes. 10 (2003) 173-180
15. Cruciani V., and Mikalsen S.-O. The connexin gene family in mammals. Biol. Chem. 386 (2005) 325-332
16. Bondarev I., Vine A., and Bertram J.S. Cloning and functional expression of a novel human connexin-25 gene. Cell Commun. Adhes. 8 (2001) 167-171
17. Levin M., and Mercola M. Expression of connexin 30 in Xenopus embryos and its involvement in hatching gland function. Dev. Dyn. 219 (2000) 96-101
18. Landesman Y., Postma F.R., Goodenough D.A., and Paul D.L. Multiple connexins contribute to intercellular communication in the Xenopus embryo. J. Cell Sci. 116 (2003) 29-38
19. Pollet N., Muncke N., Verbeek B., Li Y., Fenger U., Delius H., and Niehrs C. An atlas of differential gene expression during early Xenopus embryogenesis. Mech. Dev. 122 (2005) 365-439
20. Bruzzone R., White T.W., and Paul D.L. Connections with connexins: the molecular basis of direct intercellular signaling. Eur. J. Biochem. 238 (1996) 1-27
21. Barrio L.C., Suchyna T., Bargiello T., Xu L.X., Roginski R.S., Bennett M.V.L., and Nicholson B.J. Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage. Proc. Natl. Acad. Sci. USA 88 (1991) 8410-8414
22. Valiunas V., Niessen H., Willecke K., and Weingart R. Electrophysiological properties of gap junction channels in hepatocytes isolated from connexin32-deficient and wild-type mice. Pflügers Arch. 437 (1999) 846-856
23. Rubin J.B., Verselis V.K., Bennett M.V.L., and Bargiello T.A. A domain substitution procedure and its use to analyze voltage dependence of homotypic gap junctions formed by connexins 26 and 32. Proc. Natl. Acad. Sci. USA 89 (1992) 3820-3824
24. Valiunas V., Manthey D., Vogel R., Willecke K., and Weingart R. Biophysical properties of mouse connexin30 gap junction channels studied in transfected human HeLa cells. J. Physiol. 519 (1999) 631-644
25. Krogh A., Larsson B., von Heijne G., and Sonnhammer E.L.L. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 (2001) 567-580