A selective chromogenic agar that distinguishes Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis

Journal of Food Protection

Volumn 69, Issue 8, 2006, Pages 2002-2006

  Juergensmeyer, Margaret A ^a   Gingras, Bruce A ^a   Restaino, Lawrence ^b   Frampton, Elon W ^b  

^a IIT RESEARCH INSTITUTE   (United States)

^b R and F Laboratories   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS (BACTERIUM); BACILLUS ANTHRACIS; BACILLUS CEREUS; BACILLUS CEREUS GROUP; BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS);

AGAR; PHOSPHATIDYLCHOLINE SPECIFIC PHOSPHOLIPASE C; PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE C; PHOSPHOLIPASE C;

ARTICLE; BACILLUS ANTHRACIS; BACILLUS CEREUS; BACILLUS THURINGIENSIS; BACTERIAL COUNT; CHEMISTRY; ENZYMOLOGY; FOOD CONTAMINATION; FOOD CONTROL; HEMOLYSIS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; SPECIES DIFFERENCE;

AGAR; BACILLUS ANTHRACIS; BACILLUS CEREUS; BACILLUS THURINGIENSIS; COLONY COUNT, MICROBIAL; FOOD CONTAMINATION; FOOD MICROBIOLOGY; HEMOLYSIS; PHOSPHOLIPASE C; SPECIES SPECIFICITY;

EID: 33748344465     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-69.8.2002     Document Type: Article

References (16)

1. Agaisse, H., M. Gominet, O. A. Okstad, A. B. Kolsto, and D. Lereclus. 1999. PlcR is a pliotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis. Mol. Microbiol. 32:1043-1053.
2. Centers for Disease Control and Prevention. 2001. Basic laboratory protocols for the presumptive identification of anthrax. U.S. Department of Health and Human Services, Atlanta.
3. Dragon, D. C., and R. P. Rennie. 2001. Evaluation of spore extraction and purification methods for selective recovery of viable Bacillus anthracis spores. Lett. Appl. Microbiol. 33:100-105.
4. Guttmann, D. M., and D. J. Ellar. 2000. Phenotypic and genotypic comparisons of 23 strains form the Bacillus cereus complex for a selection of known and putative B. thuringiensis virulence factors. FEMS Microbiol. Lett. 188:7-13.
5. Hanes, D. E., M. L. Saylor, B. D. Tall, M. H. Kothary, E. A. Tall, D. H. Burr, and L. Restaino. 2004. Optimizing conditions for the isolation and identification of Bacillus anthracis from food, p. 490. Abstr. 104th Gen. Meet. Am. Soc. Microbiol. 2004. American Society for Microbiology, Washington, D.C.
6. Higgins, J. A., M. Cooper, L. Schroeder-Tucker, S. Black, D. Miller, J. S. Karns, E. Manthey, R. Breeze, and M. L. Perdue. 2003. A field investigation of Bacillus anthracis contamination of U.S. Department of Agriculture and other Washington, D.C., buildings during the anthrax attack of October 2001. Appl. Environ. Microbiol. 69:593-599.
7. Kolstø, A.-B. 2000. Personal communication.
8. Luna, V. A., K. K. Peak, W. O. Veguilla, F. Reeves, L. Heberlein-Larson, A. C. Cannons, P. Amuso, and J. Catanni. 2005. Use of two selective media and a broth motility test can aid in identification or exclusion of Bacillus anthracis. J. Clin. Microbiol. 43:4336-4341.
9. Mollby, R. 1978. Bacterial phospholipases, p. 367-424. In J. Jelgaszwicz and T. Wadstrom (ed.). Bacterial toxins and cell membranes. Academic Press, New York.
10. Morse, S. A., R. B. Kellogg, S. Perry, R. F. Meyer, D. Bray, D. Nichelson, and J. M. Miller. 2003. Detecting biothreat agents: the laboratory response network. Am. Soc. Microbiol. News 69:433-437.
11. Peng, H., V. Ford, E. W. Frampton, L. Restaino, L. A. Shelef, and H. Spitz. 2001. Isolation and enumeration of Bacillus cereus from foods on a novel chromogenic plating medium. Food Microbiol. 18:231-238.
12. Perdue, M. L., J. Karns, J. Higgins, and J. A. van Kessel. 2003. Detection and fate of Bacillus anthracis (Sterne) vegetative cells and spores added to bulk tank milk. J. Food Prot. 66:2349-2354.
13. Popovic, T., A. Hoffmaster, J. W. Ezzell, T. G. Abshire, and J. E. Brown. 2005. Validation of methods for confirmatory identification of presumptive isolates of Bacillus anthracis. J. AOAC Int. 88:175-177.
14. Sasser, M., C. Kunitsky, G. Jackoway, J. W. Ezzell, J. D. Teska, B. Harper, and S. Parker. 2005. Identification of Bacillus anthracis from culture using gas chromatographic analysis of fatty acid methyl esters. J. AOAC Int. 88:178-181.
15. Schabert, G., and L. Restaino. July 2002. Potentially fluorogenic compounds. U.S. patent 6,416,970.
16. Slamti, L., S. Perchat, M. Gominet, G. Vilas-Bôas, A. Fouet, M. Mock, V. Sanchis, J. Chaufaux, M. Gohar, and D. Lereclus. 2004. Distinct mutations in PlcR explain why some strains of the Bacillus cereus group are nonhemolytic. J. Bacteriol. 186:3531-3538.