A chemiluminescence-based reporter system to monitor nonsense-mediated mRNA decay

Biochemical and Biophysical Research Communications

Volumn 349, Issue 1, 2006, Pages 186-191

  Boelz, Stephanie ^a,b   Neu Yilik, Gabriele ^a,c   Gehring, Niels H ^a,c   Hentze, Matthias W ^a,b   Kulozik, Andreas E ^a,c  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

Chemical biology; Luciferase; Nonsense mediated mRNA decay; Reporter assay; UPF1 phosphorylation; Wortmannin

Indexed keywords

LUCIFERASE; MESSENGER RNA; PHOSPHATIDYLINOSITOL 3 KINASE INHIBITOR; PHOSPHOPROTEIN; UNCLASSIFIED DRUG; UPF1 PROTEIN; WORTMANNIN;

ARTICLE; BIOLOGY; CARBOXY TERMINAL SEQUENCE; CHEMICAL BIOLOGY; CHEMOLUMINESCENCE; CONTROLLED STUDY; DOSE RESPONSE; GENE EXPRESSION; GENETIC DISORDER; HUMAN; HUMAN CELL; MOLECULAR SIZE; NONHUMAN; NONSENSE MEDIATED MESSENGER RNA DECAY; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RENILLA; REPORTER GENE; RNA DEGRADATION; RNA INTERFERENCE; RNA PROCESSING; RNA TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; STOP CODON;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANDROSTADIENES; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; GENE EXPRESSION REGULATION; GENES, REPORTER; GENETIC TECHNIQUES; HELA CELLS; HUMANS; LUMINESCENCE; PROTEIN STRUCTURE, TERTIARY; RNA INTERFERENCE; RNA, MESSENGER; TRANS-ACTIVATORS; TRANSFECTION;

MAMMALIA; RENILLA;

EID: 33748324657     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.08.017     Document Type: Article

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