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Volumn 125, Issue 8, 2006, Pages

Fluorescence correlation spectroscopy analysis of segmental dynamics in actin filaments

Author keywords

[No Author keywords available]

Indexed keywords

COMPUTATION THEORY; FLUORESCENCE; IMAGE SEGMENTATION; MATHEMATICAL MODELS; POLYMERS; SPECTROSCOPIC ANALYSIS;

EID: 33748267883     PISSN: 00219606     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.2244550     Document Type: Article
Times cited : (21)

References (40)
  • 33
    • 33748252362 scopus 로고    scopus 로고
    • note
    • For fluorescence microscopy observations the whole filament has to be visualized. Thus the samples are polymerized from totally fluorescent seeds as in sample 1 procedure. However, the polymerization mixture had 1:9 fluorescent to nonfluorescent G-actin. This preparation results in overall labeled filaments with bright spots marking the places of former seeds. For microscopy visualization and length measurements the solutions were diluted to 0.1 μM actin concentration (ten times smaller than for FCS measurements).
  • 37
  • 39
    • 33748281443 scopus 로고    scopus 로고
    • note
    • We note here that for the weakly entangled solutions such as our samples, it is might be more appropriate to use mesh size ξ as an upper cutoff when evaluating hydrodynamic interactions and to use polymer length l for the calculation of eigenmodes in the theory of Harnau et al. However, there is almost no difference in the net result for monomers' MSD when separating ξ and l length scales as compared to the calculation with a single parameter l as put forward in the original theory (Ref. 12).
  • 40
    • 33748262708 scopus 로고    scopus 로고
    • note
    • 2(t)〉. While we are ambivalent on this issue, both types of comparison lead to essentially the same conclusions.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.