Molecular and functional characterization of a cDNA encoding fructan:fructan 6G-fructosyltransferase (6G-FFT)/fructan:fructan 1-fructosyltransferase (1-FFT) from perennial ryegrass (Lolium perenne L.)

Journal of Experimental Botany

Volumn 57, Issue 11, 2006, Pages 2719-2734

  Lasseur, Bertrand ^a   Lothier, Jérémy ^a   Djoumad, Abdelmadjid ^b   De Coninck, Barbara ^d   Smeekens, Sjef ^c   Van Laere, André ^d   Morvan Bertrand, Annette ^a   Van Den Ende, Wim ^d   Prud'homme, Marie Pascale ^a  

^a UNIVERSITÉ DE CAEN   (France)

^b UNIVERSITÉ DE SHERBROOKE   (Canada)

^c UTRECHT UNIVERSITY   (Netherlands)

^d UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Fructan; Fructan:fructan 6G fructosyltransferase; Gene expression; Heterologous expression; Lolium perenne; Pichia pastoris; Sucrose:sucrose 1 fructosyltransferase

Indexed keywords

FRUCTAN 6G-FRUCTOSYLTRANSFERASE; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; LOLIUM PERENNE; PICHIA PASTORIS; SUCROSE 1 FRUCTOSYLTRANSFERASE;

ENZYME KINETICS; ENZYMES; GENES; MOLECULAR BIOLOGY; POLYSACCHARIDES; SUGAR (SUCROSE); TISSUE;

DNA;

COMPLEMENTARY DNA; FRUCTAN; FRUCTAN - FRUCTAN 6G-FRUCTOSYLTRANSFERASE; FRUCTAN FRUCTAN 6G FRUCTOSYLTRANSFERASE; GLYCOSYLTRANSFERASE; HYBRID PROTEIN; INULIN; SUCROSE; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOLOGICAL MODEL; BIOSYNTHESIS; CARBOHYDRATE METABOLISM; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETICS; GROWTH, DEVELOPMENT AND AGING; LOLIUM; METABOLISM; MOLECULAR GENETICS; PHYLOGENY; PICHIA; PLANT LEAF; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; CARBOHYDRATE METABOLISM; DNA, COMPLEMENTARY; FRUCTANS; GENE EXPRESSION REGULATION, PLANT; GENE LIBRARY; HEXOSYLTRANSFERASES; INULIN; LOLIUM; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PHYLOGENY; PICHIA; PLANT LEAVES; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SUCROSE;

ALLIUM CEPA; LOLIUM PERENNE; PICHIA PASTORIS;

EID: 33747873013     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erl034     Document Type: Article

References (55)

1. Amiard V, Morvan-Bertrand A, Billard JP, Huault C, Keller F, Prud'homme MP. 2003. Fructans, but not the sucrosyl-galactosides, raffinose and loliose, are affected by drought stress in perennial ryegrass. Plant Physiology 132, 2218-2229.
2. Bancal P, Carpita NC, Gaudillère JP. 1992. Differences in fructan accumulated in induced and field-grown wheat plants: an elongation trimming pathway for their synthesis. New Phytologist 120, 313-321.
3. Chalmers J, Johnson X, Lidgett A, Spangenberg G. 2003. Isolation and characterisation of a sucrose:sucrose 1-fructosyltransferase gene from perennial ryegrass (Lolium perenne). Journal of Plant Physiology 160, 1385-1391.
4. Chatterton NJ, Harrison PA, Thornley WR, Bennett JH. 1993a. Structure of fructan oligomers in orchardgrass (Dactylis glomerata L.). Journal of Plant Physiology 142, 552-556.
5. Chatterton NJ, Harrison PA, Thornley WR, Bennett JH. 1993b. Structure of fructan oligomers in cheatgrass (Bromus tectorum L.). New Phytologist 124, 389-396.
6. De Coninck B, Le Roy K, Francis I, Clerens S, Vergauwen R, Halliday AM, Smith SM, Van Laere A, Van den Ende W. 2005. Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities. Plant and Cell Environment 28, 432-443.
7. Duchateau N, Bortlik K, Simmen U, Wiemken A, Bancal P. 1995. Sucrose:fructan 6-fructosyltransferase, a key enzyme for diverting sucrose to fructan in barley leaves. Plant Physiology 107, 1249-1255.
8. Edelman J, Jefford TG. 1968. The mechanism of fructosan metabolism in higher plants as exemplified in Helianthus tuberosus L. New Phytologist 67, 517-531.
9. Ernst M, Chatterton NJ, Harrison PA, Matitschka G. 1998. Characterization of fructan oligomer from species of the genus Allium L. Journal of Plant Physiology 153, 53-60.
10. Fujishima M, Sakai H, Ueno K, Takahashi N, Onodera S, Benkeblia N, Shiomi N. 2005. Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo. New Phytologist 165, 513-524.
11. Gallagher JA, Cairns AJ, Pollock CJ. 2004. Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L. Journal of Experimental Botany 55, 557-569.
12. Guerrand D, Prud'homme MP, Boucaud J. 1996. Fructan metabolism in expanding leaves, mature leaf sheaths and mature leaf blades of Lolium perenne: fructan synthesis, fructosyltransferase and invertase activities. New Phytologist 134, 205-214.
13. Hendry GAF, Wallace RK. 1993. The origin, distribution and evolutionary significance of fructans. In: Suzuki M, Chatterton NJ, eds. Science and technology of fructans. Boca Raton, FL: CRC Press, 119-139.
14. Hisano H, Kanazawa K, Yoshida M, Kitamura K, Yamada T. 2004. Lolium perenne mRNA for fructan:fructan 6G-fructosyltransferase. NCBI Database AB125218.
15. Hochstrasser U, Lüscher M, De Virgilio C, Boller T, Wiemken A. 1998. Expression of a functional barley sucrosefructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris. FEBS Letters 440, 356-360.
16. Kaur N, Gupta AK. 2002. Applications of inulin and oligofructose in health and nutrition. Journal of Bioscience 27, 703-714.
17. Kawakami A, Yoshida M. 2005. Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat. Planta 223, 90-104.
18. Koops AJ, Jonker HH. 1996. Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus Colombia. II. Purification of sucrose:sucrose 1-fructosyltransferase and reconstitution of fructan synthesis in vitro with purified sucrose:sucrose 1-fructosyltransferase and fructan: fructan 1-fructosyltransferase. Plant Physiology 110, 1167-1175.
19. Lasseur B, Djoumad A, Prud'homme MP. 2002. Lolium perenne fructosyltransferase (putative 1-SST) mRNA. NCBI Database AF492836.
20. Lüscher M, Erdin C, Sprenger N, Hochstrasser U, Boller T, Wiemken A. 1996. Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus. FEBS Letters 385, 39-42.
21. Lüscher M, Nelson CJ. 1995. Fructosyltransferase activities in the leaf growth zone of tall fescue. Plant Physiology 107, 1419-1425.
22. Lüscher M, Hochstrasser U, Vogel G, Aeschbacher R, Galati V, Nelson CJ, Boller T, Wiemken A. 2000. Cloning and functional analysis of sucrose:sucrose 1-fructosyltransferase from tall fescue. Plant Physiology 124, 1217-1227.
23. Livingston III DP, Chatterton NJ, Harrison PA. 1993. Structure and quantity of fructan oligomers in oat (Avena spp.). New Phytologist 123, 725-734.
24. Miller LA, Moorby JM, Davies DR, Humphreys MO, Scollan ND, Macrae JC, Theodorou MK. 2001. Increase concentration of water soluble carbohydrate in perennial ryegrass (Lolium perenne L.). Milk production from late-lactation dairy cows. Grass and Forage Science 56, 383-394.
25. Morvan-Bertrand A, Boucaud J, Le Saos J, Prud'homme MP. 2001. Roles of the fructans from leaf sheaths and from the elongating leaf bases in the regrowth following defoliation of Lolium perenne L. Planta 213, 109-120.
26. Pavis N, Boucaud J, Prud'homme MP. 2001b. Fructans and fructan-metabolizing enzymes in leaves of Lolium perenne. New Phytologist 150, 97-109.
27. Pavis N, Chatterton NJ, Harrison PA, Baumgartner S, Praznik W, Boucaud J, Prud'homme MP. 2001a. Structure of fructans in roots and leaf tissues of Lolium perenne. New Phytologist 150, 83-95.
28. Prud'homme MP, Gonzalez B, Billard JP, Boucaud J. 1992. Carbohydrate content, fructan and sucrose enzyme activities in roots, stubble and leaves of ryegrass (Lolium perenne L.) as affected by source/sink modification after cutting. Journal of Plant Physiology 140, 282-291.
29. Rao AV. 1999. Dose-response effects of inulin and oligofructose on intestinal bifidogenesis effects. Journal of Nutrition 129, 14425-14455.
30. Ritsema T, Joling J, Smeekens J. 2003. Patterns of fructan synthesized by onion fructan:fructan 6G-fructosyltransferase expressed in tobacco BY2 cells-is fructan:fructan 1-fructosyltransferase needed in onion? New Phytologist 160, 61-67.
31. Ritsema T, Verhaar A, Vijn I, Smeekens S. 2004. Fructosyltransferase mutants specify a function for the β-fructosidase motif of the sucrose-binding box in specifying the fructan type synthesized. Plant Molecular Biology 54, 853-863.
32. Roth A, Lüscher M, Sprenger N, Boller T, Wiemken A. 1996. Fructan and fructan-metabolizing enzymes in the growth zone of barley leaves. New Phytologist 136, 73-79.
33. Schnyder H, Nelson CJ. 1989. Growth rates and assimilate partitioning in the elongation zone of tall fescue leaf blades at high and low irradiance. Plant Physiology 90, 1201-1206.
34. Schnyder H, Seo S, Rademacher IF, Kübbauch W. 1990. Spatial distribution of growth rates and of epidermal cell lengths in the elongation zone during leaf development in Lolium perenne L. Planta 181, 423-431.
35. G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.). Carbohydrate Research 96, 281-292.
36. F-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.). Carbohydrate Research 99, 157-169.
37. Shiomi N. 1989. Properties of fructosyltransferases involved in the synthesis of fructan in liliaceous plants. Journal of Plant Physiology 134, 151-155.
38. Shiomi N, Onodera S, Sakai H. 1997. Fructo-oligosaccharide content and fructosyltransferase activity during growth of onion bulbs. New Phytologist 136, 105-113.
39. Sims IM, Pollock CJ, Horgan R. 1992. Structural analysis of oligomeric fructans from excised leaves of Lolium temulentum. Phytochemistry 31, 2989-2992.
40. Smouter H, Simpson RJ. 1991. Fructan metabolism in leaves of Lolium rigidum Gaudin. I. Synthesis of fructan. New Phytologist 119, 509-516.
41. Sprenger N, Bortlik K, Brandt A, Boller T, Wiemken A. 1995. Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley. Proceedings of the National Academy of Sciences, USA 92, 11652-11656.
42. St John JA, Bonnett GD, Simpson RJ, Tanner GJ. 1997a. A fructan:fructan fructo-syltransferase activity from Lolium rigidum. New Phytologist 135, 249-257.
43. St John JA, Sims IM, Bonnett GD, Simpson RJ. 1997b. Identification of products formed by a fructan:fructan fructosyltransferase activity from Lolium rigidum. New Phytologist 135, 249-257.
44. G-fructosyltransferase from asparagus (Asparagus officinalis). New Phytologist 165, 813-824.
45. Van den Ende W, Van Laere A. 1996. Fructan synthesizing and degrading activities in chicory roots (Cichorium intybus L.) during growth, storage forcing. Journal of Plant Physiology 149, 43-50.
46. Van den Ende W, Michiels A, Van Wonterghem D, Clerens SP, De Roover J, Van Laere AJ. 2001. Defoliation induces fructan 1-exohydrolase II in witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes. Plant Physiology 126, 1186-1195.
47. Verhaest M, Van den Ende W, Yoshida M, Le Roy K, Peeraer Y, Sansen S, De Ranter CJ, Van Laere A, Rabijns A. 2004. Crystallization and preliminary X-ray study of fructan 1-exohydrolase IIa from Cichorium intybus. Acta Crystallographica D60, 553-554.
48. Verhaest M, Van den Ende W, Le Roy K, De Ranter CJ, Van Laere A, Rabijns A. 2005. X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus. The Plant Journal 41, 400-411.
49. Vijn I, Van Dijken A, Sprenger N, Van Dun K, Weisbeek P, Wiemken A, Smeekens S. 1997. Fructan of the inulin neoseries is synthesized in transgenic chicory plants (Cichorium intybus L.) harbouring onion (Allium cepa L.) fructan:fructan 6G-fructosyltransferase. The Plant Journal 11, 387-398.
50. Wei JZ, Chatterton NJ. 2001. Fructan biosynthesis and fructosyltransferase evolution: the expression of the 6-SFT (sucrose: fructan 6-fructosyltransferase) gene in crested wheatgrass (Agropyron cristatum). Journal of Plant Physiology 158, 1203-1213.
51. Wei JZ, Chatterton NJ, Larson SR. 2001. Expression of sucrose: fructan 6-fructosyltransferase (6-SFT) and myo-inositol 1-phosphate synthase (MIPS) genes in barley (Hordeum vulgare) leaves. Journal of Plant Physiology 158, 635-643.
52. Wei JZ, Chatterton NJ, Harrison PA, Wang RR-C, Larson SR. 2002. Characterization of fructan biosynthesis in big bluegrass (Poa secunda). Journal of Plant Physiology 159, 705-715.
53. Weyens G, Ritsema T, Van Dun K, et al. 2004. Production of tailor-made fructans in sugar beet by expression of onion fructosyltransferase genes. Plant Biotechnology Journal 2, 321-327.
54. Wiemken A, Sprenger N, Boller T. 1995. Fructan: an extension of sucrose by sucrose. In: Pontis HG, Salerno GL, Echeverria EJ, eds. Sucrose metabolism, biochemistry, physiology and molecular biology. Rockville, MD: American Society of Plant Physiologists, 178-189.
55. Wilhelm WW, Nelson CJ. 1978. Leaf growth, leaf aging and photosynthetic rate of tall fescue genotype. Crop Science 18, 769-772.