Molecular characterization and expression of a cDNA encoding fructan:fructan 6G-fructosyltransferase from asparagus (Asparagus officinalis)

New Phytologist

Volumn 165, Issue 3, 2005, Pages 813-824

  Ueno, Keiji ^a   Onodera, Shuichi ^a   Kawakami, Akira ^b   Yoshida, Midori ^b   Shiomi, Norio ^a  

^a RAKUNO GAKUEN UNIVERSITY   (Japan)

^b NATIONAL AGRICULTURAL RESEARCH CENTER FOR HOKKAIDO REGION   (Japan)

Author keywords

Asparagus (Asparagus officinalis); Fructan; Fructan:fructan 6 G fructosyltransferase (6G FFT); Fructosyltransferase; Inulin neoseries; Pichia pastoris

Indexed keywords

COMPLEMENTARY DNA; FRUCTAN - FRUCTAN 6G-FRUCTOSYLTRANSFERASE; FRUCTAN FRUCTAN 6G FRUCTOSYLTRANSFERASE; GLYCOSYLTRANSFERASE; PLANT DNA;

ENZYME; GENE EXPRESSION;

AMINO ACID SEQUENCE; ARTICLE; ASPARAGUS; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ASPARAGUS PLANT; CONSERVED SEQUENCE; DNA, COMPLEMENTARY; DNA, PLANT; GENE EXPRESSION; HEXOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEQUENCE HOMOLOGY, AMINO ACID;

ASPARAGUS; ASPARAGUS OFFICINALIS; PICHIA PASTORIS;

EID: 14244254994     PISSN: 0028646X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-8137.2004.01294.x     Document Type: Article

References (44)

1. Darwen CWE, John P. 1989. Localisation of the enzymes of fructan metabolism in vacuoles isolated by a mechanical method from tubers of Jerusalem artichoke (Helianthus tuberosus L.). Plant Physiology 89: 658-663.
2. Edelman J, Jefford T. 1968. The mechanism of fructosan metabolism in higher plants as exemplified in Helianthus tuberosus. New Phytologist 67: 517-531.
3. Frehner M, Keller F, Wiemken A. 1984. Localisation of fructan metabolism in the vacuoles isolated from protoplasts of Jerusalem artichoke. Journal of Plant Physiology 116: 197-208.
4. Fujishima M, Sakai H, Ueno K, Takahashi N, Onodera S, Benkeblia N, Shiomi N. 2005. Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructooligosaccharides in vivo. New Phytologist 165: doi:10.1111/j.1469-8137.2004.01231.x
5. de Halleux S, van Cutsem P. 1997. Cloning and sequencing of the 1-SST cDNA from chicory root (accession, U81520) (PGR97-036). Plant Physiology 113: 1003.
6. Hellwege EM, Gritscher D, Willmitzer L, Heyer AG. 1997. Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose: sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs. Plant Journal 12: 1057-1065.
7. Hellwege EM, Raap M, Gritscher D, Willmitzer L, Heyer AG. 1998. Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1-FFT cDNAs. FEBS Letters 427: 25-28.
8. Hendry GAF. 1993. Evolutionary origins and natural functions of fructans - a climatological, biogeographic and mechanistic appraisal. New Phytologist 123: 3-14.
9. Hendry GAF, Wallace RK. 1993. The origin, distribution, and evolutionary significance of fructans. In: Suzuki M, Chatterton NJ, eds. Science and technology of fructans. Boca Raton, FL, USA: CRC Press, 119-139.
10. Henrissat B. 1991. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochemical Journal 280: 309-316.
11. Hochstrasser U, Lüscher M, De Virgilio C, Boller T, Wiemken A. 1998. Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris. FEBS Letters 440: 356-360.
12. Kawakami A, Yoshida M. 2002. Molecular characterization of sucrose: sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase associated with fructan accumulation in winter wheat during cold hardening. Bioscience, Biotechnology, and Biochemistry 66: 2297-2305.
13. Koops AJ, Jonker HH. 1994. Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus 'Columbia'. I. Fructan: fructan fructosyltransferase. Journal of Experimental Botany 45: 1623-1631.
14. Koops AJ, Jonker HH. 1996. Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus Columbia. II. Purification of sucrose:sucrose 1-fructosyltransferase and reconstitution of fructan synthesis in vitro with purified sucrose: sucrose 1-fructosyltransferase and fructan:fructan 1-fructosyltransferase. Plant Physiology 110: 1167-1175.
15. Lüscher M, Frehner M, Nösberger J. 1993. Purification and characterization of fructan:fructan fructosyltransferase from Jerusalem artichoke (Helianthus tuberosus L.). New Phytologist 123: 717-724.
16. Lüscher M, Erdin C, Sprenger N, Hochstrasser U, Boller T, Wiemken A. 1996. Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus. FEBS Letters 385: 39-42.
17. Pilon-Smits EAH, Ebskamp MJM, Paul MJ, Jeuken MJW, Weisbeek PJ, Smeekens SCM. 1995. Improved performance of transgenic fructan-accumulating tobacco under drought stress. Plant Physiology 107: 125-130.
18. Raccuia SA, Melilli MG. 2004. Cynara cardunculus L., a potential source of inulin in the Mediterranean environment: screening of genetic variability. Australian Journal of Agricultural Research 55: 693-698.
19. Reddy VA, Maley F. 1990. Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis. Journal of Biological Chemistry 265: 10817-10820.
20. Reddy A, Maley F. 1996. Studies on identifying the catalytic role of Glu-204 in the active site of yeast invertase. Journal of Biological Chemistry 271: 13953-13958.
21. Ritsema T, Joling J, Smeekens S. 2003. Patterns of fructan synthesized by onion fructan: fructan 6G-fructosyltransferase expressed in tobacco BY2 cells - is fructan:fructan 1-fructosyltransferase needed in onion? New Phytologist 160: 61-67.
22. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, NY, USA: Cold. Spring Harbor Press.
23. G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.). Carbohydrate Research 96: 281-292.
24. F-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.). Carbohydrate Research 99: 157-169.
25. G-fructosyltransferase. Carbohydrate Research 106: 166-169.
26. Shiomi N. 1989. Properties of fructosyltransferases involved in the synthesis of fructan in Liliaceous plants. Journal of Plant Physiology 134: 151-155.
27. Shiomi N, Izawa M. 1980. Purification and characterization of sucrose: sucrose 1-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.). Agricultural and Biological Chemistry 44: 603-614.
28. Shiomi N, Yamada J, Izawa M. 1976. Isolation and identification of fructooligosaccharides in roots of asparagus (Asparagus officinalis L.). Agricultural and Biological Chemistry 40: 567-575.
29. Shiomi N, Yamada J, Izawa M. 1979. A novel pentasaccharide in the roots of asparagus (Asparagus officinalis L.). Agricultural and Biological Chemistry 43: 1375-1377.
30. Shiomi N, Onodera S, Chatterton NJ, Harrison PA. 1991. Separation of fructooligosaccharide isomers by anion-exchange chromatography. Agricultural and Biological Chemistry 55: 1427-1428.
31. Shiomi N, Onodera S, Sakai H. 1997. Fructo-oligosaccharide content and fructosyltransferase activity during growth of onion bulbs. New Phytologist 136: 105-113.
32. Sprenger N, Bortlik K, Brandt A, Boller T, Wiemken A. 1995. Purification, cloning, and functional expression of sucrose: fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley. Proceedings of the National Academy of Sciences, USA 92: 11652-11656.
33. Suzuki M. 1993. Fructans in the crop production and preservation. In: Suzuki M, Chatterton NJ, eds. Science and technology of fructans. Boca Raton, FL. USA: CRC Press, 227-255.
34. Takeda H, Sato K, Kinoshita S, Sasaki H. 1994. Production of 1-kestose by Scopulariopsis brevicaulis. Journal of Fermentation and Bioengineering 77: 386-389.
35. Unger C, Hardegger M, Lienhard S, Sturm A. 1994. cDNA cloning of carrot (Daucus carota) soluble acid β-fructofuranosidases and comparison with the cell wall isoenzyme. Plant Physiology 104: 1351-1357.
36. van den Ende W, van Wonterghem D, Verhaert P, Dewil E, van Laere A. 1996. Purification and characterization of fructan: fructan fructosyltransferase from chicory roots (Cichorium intybus L.). Planta 199: 493-502.
37. van den Ende W, Michiels A, De Roover J, Verhaert P, van Laere A. 2000. Cloning and functional analysis of chicory root fructan 1-exohydrolase I (1-FEH I): a vacuolar enzyme derived from a cell-wall invertase ancestor? Mass fingerprint of the 1-FEH I enzyme. Plant Journal 24: 447-456.
38. van den Ende W, Michiels A, van Wonterghem D, Clerens SP, De Roover J, van Laere AJ. 2001. Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzyme. Plant Physiology 126: 1186-1195.
39. van der Meer IM, Koops AJ, Hakkert JC, van Tunen AJ. 1998. Cloning of the fructan biosynthesis pathway of Jerusalem artichoke. Plant Journal 15: 489-500.
40. Vijn I, Smeekens S. 1999. Fructan: more than a reserve carbohydrate? Plant Physiology 120: 351-359.
41. G-fructosyltransferase. Plant Journal 11: 387-398.
42. Wei JZ, Chatterton NJ. 2001. Fructan biosynthesis and fructosyltransferase evolution: Expression of the 6-SFT (sucrose:fructan 6-fructosyltransferase) gene in crested wheatgrass (Agropyron cristatum). Journal of Plant Physiology 158: 1203-1213.
43. Wiemken A, Sprenger N, Boller T. 1995. Fructan extension of sucrose by sucrose. In: Pontis HG, Salerno GL, Echeverria EJ, eds. Sucrose metabolism, biochemistry, physiology and molecular biology. Rockville, MD, USA: American Society of Plant Physiologists, 178-189.
44. Yoshida M, Abe J, Moriyama M, Kuwabara T. 1998. Carbohydrate levels among winter wheat cultivars varying in freezing tolerance and snow mold resistance during autumn and winter. Physiologia Plantarum 103: 8-16.