Development of an expression vector for Metarhizium anisopliae based on the tef-1α homologous promoter

Applied Microbiology and Biotechnology

Volumn 72, Issue 3, 2006, Pages 521-528

  Nakazato, Luciano ^a   Dutra, Valéria ^a   Broetto, Leonardo ^a   Staats, Charley Christian ^a   Vainstein, Marilene Henning ^a   Schrank, Augusto ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ENTHOMOPATHOGENIC FUNGUS; METARHIZIUM ANISOPLIAE; TRANSLATION ELONGATION FACTOR; WILD-TYPE STRAIN;

BIOASSAY; BIOMARKERS; DNA; FUNGI; GENES; MICROORGANISMS;

PROTEINS;

DNA FRAGMENT; ELONGATION FACTOR 1ALPHA; GREEN FLUORESCENT PROTEIN; PHOSPHINOTHRICIN;

BIOASSAY; BIOMARKER; BIOTRANSFORMATION; DNA; FUNGUS; GENE EXPRESSION; PROTEIN; TICK; VIRULENCE;

AMINO ACID SEQUENCE; ARTICLE; BAR GENE; BIOASSAY; BOOPHILUS MICROPLUS; CONTROLLED STUDY; EXPRESSION VECTOR; FLUORESCENCE ANALYSIS; FUNGAL GENE; FUNGAL VIRULENCE; GENE CONSTRUCT; GENETIC TRANSFORMATION; METARHIZIUM ANISOPLIAE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; SEQUENCE ANALYSIS; START CODON;

ANIMALS; ASCOMYCOTA; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FEMALE; FUNGAL PROTEINS; GENES, FUNGAL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; IXODIDAE; MOLECULAR SEQUENCE DATA; PEPTIDE ELONGATION FACTORS; PHYLOGENY; PROMOTER REGIONS (GENETICS); PROMOTER REGIONS, GENETIC; TRANSFORMATION, GENETIC;

ACARI; BOOPHILUS MICROPLUS; FUNGI; METARHIZIUM ANISOPLIAE;

EID: 33747728874     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0292-3     Document Type: Article

References (43)

1. Ackermann R, Brack C (1996) A strong ubiquitous promoter-enhancer for development and aging of Drosophila melanogaster. Nucleic Acids Res 24:2452-2453
2. Arruda W, Lubeck I, Schrank A, Vainstein MH (2005) Morphological alterations of Metarhizium anisopliae during penetration of Boophilus microplus ticks. Exp Appl Acarol 37:231-244
3. Bagga S, Hu G, Screen SE, St. Leger RJ (2004) Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliae. Gene 324:159-169
4. Bidochka MJ, St. Leger RJ (1997) Mechanisms of deuteromycete fungal infections in grasshoppers and locusts: an overview. Mem Entomol Soc Can 171:213-224
5. Bogo MR, Vainstein MH, Aragao FJ, Rech E, Schrank A (1996) High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus Metarhizium anisopliae. FEMS Microbiol Lett 142:123-127
6. Cavallius J, Merrick WC (1998) Site-directed mutagenesis of yeast eEF1A. Viable mutants with altered nucleotide specificity. J Biol Chem 273:28752-28758
7. Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5:213-218
8. Clarkson J, Charnley K (1996) New insights into the mechanisms of fungal pathogenesis in insects. Trends Microbiol 4:197-203
9. Dutra V, Nakazato L, Broetto L, Schrank IS, Vainstein MH, Schrank A (2004) Application of representational difference analysis to identify sequence tags expressed by Metarhizium anisopliae during the infection process of the tick Boophilus microplus cuticle. Res Microbiol 155:245-251
10. Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y (2005) Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol 71:363-370
11. Fernandez-Abalos JM, Fox H, Pitt C, Wells B, Doonan JH (1998) Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus, Aspergillus nidulans. Mol Microbiol 1:121-130
12. Frazzon AP, da Silva VJI, Masuda A, Schrank A, Vainstein MH (2000) In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Vet Parasitol 94:117-125
13. Freimoser FM, Screen S, Bagga S, Hu G, St. Leger RJ (2003) Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts. Microbiology 149:239-247
14. Goettel MS, St. Leger RJ, Bhairi S, Jung MK, Oakley BR, Roberts DW, Staples RC (1990) Pathogenicity and growth of Metarhizium anisopliae stable transformed to benomyl resistance. Curr Genet 17:129-132
15. Hu G, St. Leger RJ (2002) Field studies using a recombinant mycoinsecticide (Metarhizium anisopliae) reveal that it is rhizosphere competent. App Environ Microbiol 68:6383-6387
16. Huang B, Li C-R, Li Z-G, Fan M-Z, Li Z-Z (2002) Molecular identification of the teleomorph of Beauveria bassiana. Mycotaxon 81:229-236
17. Ichi-ishi A, Inoue H (1995) Cloning, nucleotide sequence, and expression of tef-1, the gene encoding translation elongation factor 1 alpha (EF-1 alpha) of Neurospora crassa. Jpn J Genet 70:273-287
18. Kassa A, Stephan D, Vidal S, Zimmermann G (2004) Laboratory and field evaluation of different formulations of Metarhizium anisopliae var. acridum submerged spores and aerial conidia for the control locusts and grasshoppers. Biocontrol 49:63-81
19. Kitamoto N, Matsui J, Kawai Y, Kato A, Yoshino S, Ohmiya K, Tsukagoshi N (1998) Utilization of the TEF1-alpha gene (TEF1) promoter for expression of polygalacturonase genes, pgaA and pgaB, in Aspergillus oryzae. Appl Microbiol Biotechnol 50:85-92
20. Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283-292
21. Krieger de Moraes C, Schrank A, Vainstein MH (2003) Regulation of extracellular chitinases and proteases in the entomopathogen and acaricide Metarhizium anisopliae. Curr Microbiol 46:205-210
22. Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244-1245
23. Kupfer DM, Drabenstot SD, Buchanan KL, Lai H, Zhu H, Dyer DW, Roe BA, Murphy JW (2004) Introns and splicing elements of five diverse fungi. Eukaryot Cell 3:1088-1100
24. Lord JC (2005) From Metchnikoff to Monsanto and beyond: the path of microbial control. J Invertebr Pathol 89:19-29
25. Mita K, Morimyo M, Ito K, Sugaya K, Ebihara K, Hongo E, Higashi T, Hirayama Y, Nakamura Y (1997) Comprehensive cloning of Schizosaccharomyces pombe genes encoding translation elongation factors. Gene 187:259-266
26. Muller S, Sandal T, Kamp-Hansen P, Dalboge H (1998) Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 14:1267-1283
27. Nishimura Y, Kamei A, Uno-Furuta S, Tamaki S, Kim G, Adachi Y, Kuribayashi K, Matsuura Y, Miyamura T, Yasutomi Y (1999) A single immunization with a plasmid encoding hepatitis C virus (HCV) structural proteins under the elongation factor 1-alpha promoter elicits HCV-specific cytotoxic T-lymphocytes (CTL). Vaccine 18:675-680
28. Pinto AS, Barreto CC, Schrank A, Ulhoa CJ, Vainstein MH (1997) Purification and characterization of an extracellular chitinase from the entomopathogen Metarhizium anisopliae. Can J Microbiol 43:322-327
29. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56:117-124
30. Punt PJ, Greaves PA, Kuyvenhoven A, van Deutekom JC, Kinghorn JR, Pouwels PH, van den Hondel CA (1991) A twin-reporter vector for simultaneous analysis of expression signals of divergently transcribed, contiguous genes in filamentous fungi. Gene 104:119-122
31. Punt PJ, van Biezen N, Conesa A, Albers A, Mangnus J, van den Hondel C (2002) Filamentous fungi as cell factories for heterologous protein production. Trends Biotechnol 20:200-206
32. Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
33. Schilde C, Wostemeyer J, Burmester A (2001) Green fluorescent protein as a reporter for gene expression in the mucoralean fungus Absidia glauca. Arch Microbiol 175:1-7
34. Schillberg S, Tiburzy R, Fischer R (2000) Transient transformation of the rust fungus Puccinia graminis f. sp. tritici. Mol Gen Genet 262:911-915
35. Scholte E-J, Ng'habi K, Kihonda J, Takken W, Paaijmans K, Abdulla S, Killeen GF, Knols BGJ (2005) An entomopathogenic fungus for control of adult African malaria mosquitoes. Science 308:1641-1642
36. Silva MV, Santi L, Staats CC, Costa AM, Colodel EM, Driemeier D, Vainstein MH, Schrank A (2005) Cuticle-induced endo/ exoacting chitinase CHIT30 from Metarhizium anisopliae is encoded by an ortholog of the chi3 gene. Res Microbiol 156:382-392
37. Stensrud O, Hywel-Jones NL, Schumacher T (2005) Towards a phylogenetic classification of Cordyceps: ITS nrDNA sequence data confirm divergent lineages and paraphyly 1. Mycol Res 109:41-56
38. St. Leger R, Shimizu S, Joshi L, Bidochka MJ, Roberts DW (1995) Co-transformation of Metarhizium anisopZiae by electroporation or using the gene gun to produce stable GUS transformants. FEMS Microbiol Lett 131:289-294
39. St. Leger R, Joshi L, Bidochka MJ, Roberts DW (1996) Construction of an improved mycoinsecticide overexpressing a toxic protease. Proc Natl Acad Sci U S A 93:6349-6354
40. Thompson C, Movva NR, Tizard R, Cramei R, Davies JE, Lauwereys M, Botterman J (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J 6:2519-2523
41. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882
42. Vanden Wymelenberg AJ, Cullen D, Spear RN, Schoenike B, Andrews JH (1997) Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces. Biotechniques 23:686-690
43. Wang C, Hu G, St. Leger RJ (2005) Differential gene expression by Metarhizium anisopliae growing in root exudate and host (Manduca sexta) cuticle or hemolymph reveals mechanisms of physiological adaptation. Fungal Genet Biol 42:704-718