Utilization of n-alkanes by a newly isolated strain of Acinetobacter venetianus: The role of two AlkB-type alkane hydroxylases

Applied Microbiology and Biotechnology

Volumn 72, Issue 2, 2006, Pages 353-360

  Throne Holst, Mimmi ^a   Markussen, Sidsel ^b   Winnberg, Asgeir ^b   Ellingsen, Trond E ^b   Kotlar, Hans Kristian ^c   Zotchev, Sergey B ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b SINTEF MATERIALS AND CHEMISTRY   (Norway)

^c STATOIL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

ACINETOBACTER; ACINETOBACTER VENETIANUS; N-ALKANES; POLYMERASE CHAIN REACTION;

BACTERIA; DNA; ENZYMES; GENES; RNA;

PARAFFINS;

ALKANE; ALKANE HYDROXYLASE; DECANE; DNA FRAGMENT; OXYGENASE; PARAFFIN; RNA 16S; TETRACONTANE; UNCLASSIFIED DRUG;

BACTERIUM; CARBON; COMPARATIVE STUDY; DNA; ENZYME ACTIVITY; GROWTH RATE; ORGANIC COMPOUND; RNA;

ACINETOBACTER; ACINETOBACTER VENETIANUS; ALKMA GENE; ALKMB GENE; ARTICLE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BACTERIUM ISOLATE; ENZYME ACTIVITY; GENE; GENE AMPLIFICATION; MICROBIAL DEGRADATION; NONHUMAN; POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEQUENCE HOMOLOGY;

ACINETOBACTER; ALKANE 1-MONOOXYGENASE; ALKANES; BACTERIAL PROTEINS; ISOENZYMES; MOLECULAR SEQUENCE DATA; MUTATION; PARAFFIN; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SUBSTRATE SPECIFICITY;

ACINETOBACTER VENETIANUS; BACTERIA (MICROORGANISMS);

EID: 33747720825     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0262-9     Document Type: Article

References (29)

1. Baptist JN, Gholson RK, Coon MJ (1963) Hydrocarbon oxidation by a bacterial enzyme system: I. Products of octane oxidation. Biochim Biophys Acta 69:40-47
2. Brautaset T, Borgos SEF, Sletta H, Ellingsen TE, Zotchev SB (2003) Site-specific mutagenesis and domain substitution in the loading module of the nystatin polyketide synthase and their effects on nystatin biosynthesis in Streptomyces noursei. J Biol Chem 278:14913-14919
3. Chakrabarty AM, Chou G, Gunsalus JC (1973) Genetic regulation of the octane dissimilation plasmid in Pseudomonas. PNAS 70:1137-1140
4. q/Ptrp-lac plasmids and transposons that confer conditional phenotypes. Gene 123:17-24
5. Di Cello F, Pepi M, Baldi F, Fani R (1997) Molecular characterization of an n-alkane-degrading bacterial community and identification of a new species, Acinetobacter venetianus. Res Microbiol 148:237-249
6. Eggink G, Lageveen RG, Altenburg B, Witholt B (1987a) Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli. J Biol Chem 262:17712-17718
7. Eggink G, van Lelyveld PH, Arnberg A, Arfman N, Witteveen C, Witholt B (1987b) Structure of the Pseudomonas putida alkBAC operon. J Biol Chem 262:6400-6406
8. Hamamura N, Yeager CM, Arp DJ (2001) Two distinct mono-oxygenases for alkane oxidation in Nocardioides sp. strain CF8. Appl Environ Microbiol 67:4992-4998
9. Hara A, Baik S-H, Syutsubo K, Misawa N, Smits THM, van Beilen JB, Harayama S (2004) Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2. Environ Microbiol 6:191-197
10. Kok M, Oldenhuis R, van der Linden MPG, Raatjes P, Kingma J, van Lelyveld PH, Witholt B (1989) Pseudomonas oleovorans alkane hydroxylase gene. Sequence and expression. J Biol Chem 264:5435-5441
11. Maeng JH, Sakai Y, Tani Y, Kato N (1996) Isolation and characterization of a novel oxygenase that catalyses the first step of n-alkane oxidation in Acinetobacter sp. strain M-1. J Bacteriol 178:3695-3700
12. Maier T, Förster H-H, Asperger O, Hahn U (2001) Molecular characterization of the 56-kDA CYP153 from Acinetobacter sp. EB104. Biochem Biophys Res Commun 286:652-658
13. Minas W, Gutnick DL (1993) Isolation, characterization and sequence analysis of cryptic plasmids from Acinetobacter calcoaceticus and their use in construction of Escherichia coli shuttle plasmids. Appl Environ Microbiol 59:2807-2816
14. Rosenberg M, Bayer EA, Delarea J, Rosenberg E (1982) Role of thin fimbriae in adherence and growth of Acinetobacter calcoaceticus RAG-1 on hexadecane. Appl Environ Microbiol 44:929-937
15. 44) by an isolate, Acinetobacter sp. M-1. Biosci Biotechnol Biochem 58:2128-2130
16. Smits THM, Röthlisberger M, Witholt B, van Beilen JB (1999) Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains. Environ Microbiol 1:307-317
17. Smits THM, Balada SB, Witholt B, van Beilen JB (2002) Functional analysis of alkane hydroxylases from Gram-negative and Gram-positive bacteria. J Bacteriol 184:1733-1742
18. Tani A, Ishige T, Sakai Y, Kato N (2001) Gene structure and regulation of the alkane hydroxylase complex in Acinetobacter sp. Strain M-1. J Bacteriol 183:1819-1823
19. van Beilen JB, Panke S, Lucchini S, Franchini AG, Röthlisberger M, Witholt B (2001) Analysis of the Pseudomonas putida alkane-degradation gene cluster and flanking insertion sequences: evolution and regulation of the alk genes. Microbiology 147:1621-1630
20. van Beilen JB, Neuenschwander M, Smits THM, Roth C, Balada SB, Witholt B (2002a) Rubredoxins involved in alkane oxidation. J Bacteriol 184:1722-1732
21. van Beilen JB, Smits THM, Whyte LG, Schorcht S, Röthlisberger M, Plaggemeier T, Engesser K-H, Witholt B (2002b) Alkane hydroxylase homologues in Gram-positive strains. Environ Microbiol 4:676-682
22. van Beilen JB, Li Z, Duetz WA, Smits THM, Witholt B (2003) Diversity of alkane hydroxylase systems in the environment. Oil Gas Sci Technol 58:427-440
23. van Beilen JB, Marín MM, Smits THM, Röthlisberger M, Franchini AG, Witholt B, Rojo F (2004) Characterization of two alkane hydroxylase genes from the marine hydrocarbonoclastic bacterium Alcanivorax borkumensis. Environ Microbiol 6:264-273
24. van Beilen, JB, Smits THM, Roos FF, Brunner T, Balada SB, Röthlisberger M, Witholt B (2005) Identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases. J Bacteriol 187:85-91
25. Vaneechoutte M, Tjernberg I, Baldi F, Pepi M, Fani R, Sullivan ER, van der Toorn J, Dijkshoorn L (1999) Oil-degrading Acinetobacter strain RAG-1 and strains described as 'Acinetobacter venetianus sp. nov.' belong to the same genomic species. Res Microbiol 150:69-73
26. Van Hamme JD, Singh A, Ward OP (2003) Recent advances in petroleum microbiology. Microbiol Mol Biol Rev 67:503-549
27. Vomberg A, Klinner U (2000) Distribution of alkB genes within n-alkane-degrading bacteria. J Appl Microbiol 89:339-348
28. Whyte LG, Smits THM, Labbe D, Witholt B, Greer CW, van Beilen JB (2002) Gene cloning and characterization of multiple alkane hydroxylase systems in Rhodococcus strains Q15 and NRRL B-16531. Appl Environ Microbiol 68:5933-5942
29. Zotchev SB, Haugan K, Sekurova O, Sletta H, Ellingsen TE, Valla S (2000) Identification of a gene cluster for antibacterial polyketide-derived antibiotic biosythesis in the nystatin producer Streptomyces noursei ATCC 11455. Microbiology 146:611-619