The effect of hydrodynamic shear on 3D engineered chondrocyte systems subject to direct perfusion

Biorheology

Volumn 43, Issue 3-4, 2006, Pages 215-222

  Raimondi, Manuela T ^a   Moretti, Matteo ^a   Cioffi, Margherita ^a   Giordano, Carmen ^a   Boschetti, Federica ^a   Laganà, Katia ^a   Pietrabissa, Riccardo ^a  

^a POLITECNICO DI MILANO   (Italy)

Author keywords

Biosynthesis; Cartilage; Computational fluid dynamics; Mechanobiology; Porous biomaterials; Simulation; Tissue engineering

Indexed keywords

GLYCOSAMINOGLYCAN POLYSULFATE; POLYESTER; POLYESTERURETHAN; POLYMER; POLYURETHAN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICULAR CARTILAGE; BIOREACTOR; CARTILAGE CELL; COMPUTER ASSISTED TOMOGRAPHY; CONFERENCE PAPER; CONTROLLED STUDY; COW; CULTURE MEDIUM; DNA DETERMINATION; ELECTRON MICROSCOPY; FLOW RATE; FOAM; HYDRODYNAMICS; MECHANICAL STIMULATION; NONHUMAN; PERFUSION; QUANTITATIVE ANALYSIS; SHEAR STRESS; SIMULATION; TISSUE ENGINEERING;

ANIMALS; BIOREACTORS; CARTILAGE, ARTICULAR; CATTLE; CELL CULTURE TECHNIQUES; CHONDROCYTES; COMPUTATIONAL BIOLOGY; MECHANOTRANSDUCTION, CELLULAR; MICROSCOPY, ELECTRON, SCANNING; MODELS, BIOLOGICAL; PERFUSION; RHEOLOGY; TISSUE ENGINEERING;

EID: 33747146367     PISSN: 0006355X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (16)

1. M. Cioffi, F. Boschetti, M.T. Raimondi and G. Dubini, Modeling evaluation of the fluid-dynamic microenvironment in tissue-engineered constructs: A micro-CT based model, Biotechnol. Bioeng. 93(3) (2006), 500-510.
2. E.M. Darling and K.A. Athanasiou, Articular cartilage bioreactors and bioprocesses, Tissue Eng. 9(1) (2003), 9-26.
3. T. Davisson, R.L. Sah and A. Ratcliffe, Perfusion increases cell content and matrix synthesis in chondrocyte three-dimensional cultures, Tissue Eng. 8(5) (2002), 807-816.
4. O. Demarteau, D. Wendt, A. Braccini, M. Jakob, D. Schafer, M. Heberer and I. Martin, Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes, Biochem. Biophys. Res. Commun. 310(2) (2003), 580-588.
5. N.S. Dunkelman, M.P. Zimber, R.G. LeBaron, R. Pavelec, M. Kwan and A.F. Purchio, Cartilage production by rabbit articular chondrocytes on polyglycolic acid scaffolds in a closed bioreactor system, Biotechnol. Bioeng. 46 (1995), 299-305.
6. R.W. Farndale, C.A. Sayers and A.J. Barrett, A direct spectrophotometric microassay for sulphated glycosaminoglycans in cartilage cultures, Connective Tissue Research 9 (1982), 247-248.
7. I. Martin, D. Wendt and M. Heberer, The role of bioreactors in tissue engineering, Trends Biotechnol. 22(2) (2004), 80-86.
8. S. Mizuno, F. Allemann and J. Glowacki, Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges, J. Biomed. Mater. Res. 56(3) (2001), 368-375.
9. V.C. Mow and C.C. Wang, Some bioengineering considerations for tissue engineering of articular cartilage, Clin. Orthop. 367 (1999), S204-S223.
10. D. Pazzano, K.A. Mercier, J.M. Moran, S.S. Fong, D.D. DiBiasio, J.X. Rulfs, S.S. Kohles and L.J. Bonassar, Comparison of chondrogensis in static and perfused bioreactor culture, Biotechnol. Prog. 16(5) (2000), 893-896.
11. B. Porter, R. Zauel, H. Stockman, R. Guldberg and D. Fyhrie, 3-D computational modeling of media flow through scaffolds in a perfusion bioreactor, J. Biomech. 38(3) (2005), 543-549.
12. M.T. Raimondi, F. Boschetti, L. Falcone, G.B. Fiore, A. Remuzzi, M. Marazzi, E. Marinoni and R. Pietrabissa, Mechanobiology of engineered cartilage cultured under a quantified fluid dynamic environment, Biomech. Modell. Mechanobiol. 1 (2002), 69-82.
13. J. Rao and W.R. Otto, Fluorimetric assay for cell growth estimation, Anal. Biochem. 207 (1992), 186-192.
14. B. Saad, Y. Kuboki, M. Welti, G.K. Uhlschmid, P. Neuenschwander and U.W. Suter, DegraPol-foam: a degradable and highly porous polyesterurethane foam as a new substrate for bone formation, Artif. Organs 24(12) (2000), 939-945.
15. S. Saini and T.M. Wick, Concentric cylinder bioreactor for production of tissue engineered cartilage: effect of seeding density and hydrodynamic loading on construct development, Biotechnol. Prog. 19(2) (2003), 510-521.
16. R.M. Schinagl, M.S. Kurtis, K.D. Ellis, S. Chien and R.L. Sah, Effect of seeding duration on the strength of chondrocyte adhesion to articular cartilage, J. Orthop. Res. 17(1) (1999), 121-129.