Evaluation of human MSCs cell cycle, viability and differentiation in micromass culture

Biorheology

Volumn 43, Issue 3-4, 2006, Pages 489-496

  Yang, Jing Wei ^a,b   De Isla, Natalia ^a   Huselstein, Céline ^a   Sarda Kolopp, Marie Nathalie ^c   Li, Na ^a,b   Li, Yin Ping ^b   Jing Ping, Ou Yang ^a   Stoltz, Jean François ^a   Eljaafari, Assia ^a  

^a CNRS   (France)

^b WUHAN UNIVERSITY   (China)

^c UNIVERSITÉ DE LORRAINE   (France)

Author keywords

Cell cycle; Differentiation; Mesenchymal stem cell; TGF 1; Viability

Indexed keywords

BONE MARROW; CELL CULTURE; CELL CYCLE; CELL DIFFERENTIATION; CELL VIABILITY; CHONDROGENESIS; CONFERENCE PAPER; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; MESENCHYME CELL; MULTIPOTENT STEM CELL; PHENOTYPE;

ADULT; ANTIGENS, CD; ANTIGENS, THY-1; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL DIFFERENTIATION; CELL SURVIVAL; CHONDROCYTES; CHONDROGENESIS; HUMANS; IMMUNOPHENOTYPING; MESENCHYMAL STEM CELLS; RECEPTORS, CELL SURFACE; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 33747121930     PISSN: 0006355X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (13)

1. F.P. Barry and J.M. Murphy, Mesenchymal stem cells: clinical applications and biological characterization, Review, Int. J. Biochemistry Cell. Biology 36 (2004), 568-584.
2. C. De Bari, F. Dell'Accio and F.P. Luyten, Failure of in vitro-differentiated mesenchymal stem cells from the synovial membrane to form ectopic stable cartilage in vivo, Arthritis & Rheumatism 1 (2004), 142-150.
3. J. Diaz-Romero, J.P. Gaillard, S.P. Grogan, D. Nesic, T. Trub and P. Mainil-Varlet, Immunophenotypic analysis of human articular chondrocytes: Changes in surface markers associated with cell expansion in monolayer culture, J. Cell Physiology 202 (2005), 731-742
4. M. Jakob, O. Demarteau, D. Schafer, B. Hintermann, W. Dick, M. Heberer and I. Martin, Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formation in vitro, J. Cell. Biochem. 81(2) (2001), 368-377.
5. E.A. Jones, S.E. Kinsey, A English, R.A. Jones, L. Straszynski, D.M. Meredith, A.F. Markham, J. Andrew, P. Emery and D. McGonagle, Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells, Arthritis & Rheumatism 12 (2002), 3349-3360.
6. K.W. Kavalkovich, R.E. Boynton, J.M. Murphy and F. Barry, Chondrogenic differentiation of human mesenchymal stem cells within an alginate layer culture system, In Vitro Cell. Dev. Biol. Anim. 9 (2002), 457-466.
7. J.J. Minguell, A. Erices and P. Conget, Mesenchymal stem cells, Exp. Biol. Med. 6 (2001), 507-520.
8. H. Nakahara, J.E. Dennis, S.P. Bruder, S.E. Haynesworth, D.P. Lennon and A.I. Caplan, In vitro differentiation of bone and hypertrophic cartilage from periosteal-derived cells, Exp. Cell Res. 8 (1991), 492-503.
9. W.L. Parker, M.B. Goldring and A. Philip, Endoglin is expressed on human chondrocytes and forms a heteromeric complex with betaglycan in a ligand and type II TGFbeta receptor independent manner, J. Bone Miner. Res. 2 (2003), 289-302.
10. I. Sekiya, J.T. Vuoristo, B.L. Larson and D.J. Prockop, In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis, PNAS 99 (2002), 4397-4402.
11. K.L. Summers, J.L. O'Donnell, M.S. Hoy, M. Peart, J. Dekker, A. Rothwell and D.N. Hart, Monocyte-macrophage antigen expression on chondrocytes, J. Rheumatol. 7 (1995), 1326-1334.
12. A.D. Weston, V. Rosen, R.A. Chandraratna and T.M. Underhill, Regulation of skeletal progenitor differentiation by the BMP and retinoid signaling pathways, J. Cell Biol. 148 (2000), 679-690.
13. I. Wilmut, A.E. Schnieke, J. McWhir, A.J. Kind and K.H.S. Campbell, Viable offspring derived from fetal and adult mammalian cells, Nature 385 (1997), 810-813.