Refolding strategies for ketosteroid isomerase following insoluble expression in Escherichia coli

Biotechnology and Bioengineering

Volumn 94, Issue 6, 2006, Pages 1089-1098

  Hutchinson, Matthew H ^a   Chase, Howard A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Column; Dilution; Immobilized metal affinity chromatography; Ketosteroid isomerase; Protein; Refolding

Indexed keywords

ADSORPTION; AFFINITY CHROMATOGRAPHY; CELL IMMOBILIZATION; ESCHERICHIA COLI; PURIFICATION;

COLUMN; DILUTION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; KETOSTEROID ISOMERASE; REFOLDING;

ENZYMES;

HISTIDINE; STEROID DELTA ISOMERASE;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; CARBOXY TERMINAL SEQUENCE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DILUTION; ELUTION; ENZYME ACTIVITY; ESCHERICHIA COLI; INTERMETHOD COMPARISON; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN FOLDING;

CHROMATOGRAPHY, LIQUID; COMAMONAS TESTOSTERONI; ESCHERICHIA COLI; GENE EXPRESSION; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY; STEROID ISOMERASES;

ESCHERICHIA COLI;

EID: 33747120279     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20932     Document Type: Article

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