Age-related changes in relative expression of real-time PCR housekeeping genes in human skeletal muscle

Journal of Biomolecular Techniques

Volumn 17, Issue 2, 2006, Pages 157-162

  Touchberry, Chad D ^a   Wacker, Michael J ^a   Richmond, Scott R ^a   Whitman, Samantha A ^a   Godard, Michael P ^b  

^a UNIVERSITY OF KANSAS   (United States)

^b WESTERN ILLINOIS UNIVERSITY   (United States)

Author keywords

2 microglobulin ( 2m); Glyceraldehyde 3 phosphate dehydrogenase (GAPDH); Housekeeping genes; Real time reverse transcriptase polymerase chain reaction (RT PCR); RNA polymerase 2a (polR2a)

Indexed keywords

BETA 2 MICROGLOBULIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; RNA POLYMERASE II;

ADULT; AGED; AGING; ARTICLE; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENOMICS; HUMAN; METABOLISM; METHODOLOGY; PROTEOMICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKELETAL MUSCLE;

ADULT; AGED; AGED, 80 AND OVER; AGING; BETA 2-MICROGLOBULIN; GENE EXPRESSION REGULATION; GENOMICS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); HUMANS; MUSCLE, SKELETAL; PROTEOMICS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA POLYMERASE II; RNA, MESSENGER;

EID: 33747066769     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Janssen I, Shepard DS, Katzmarzyk PT, Roubenoff R. The healthcare costs of sarcopenia in the United States. J Am Geriatr Soc 2004;52:80-85.
2. Godard MP, Williamson DL, Trappe SW. Oral amino-acid provision does not affect muscle strength or size gains in older men. Med Sci Sports Exerc 2002;34:1126-1131.
3. Kostrominova TY, Dow DE, Dennis RG, Miller RA, Faulkner JA. Comparison of gene expression of two-month denervated, two-month stimulated-denervated and control rat skeletal muscles. Physiol Genomics 2005;22(2):227-243.
4. Lecker SH, Jagoe RT, Gilbert A, Gomes M, Baracos V, Bailey J, et al. Multiple types of skeletal muscle atrophy involve a common program of changes in gene expression. FASEB J 2004;18:39-51.
5. Trappe S, Williamson D, Godard M, Porter D, Rowden G, Costill D. Effect of resistance training on single muscle fiber contractile function in older men. J Appl Physiol 2000;89:143-152.
6. Trappe TA, Lindquist DM, Carrithers JA. Muscle-specific atrophy of the quadriceps femoris with aging. J Appl Physiol 2001;90:2070-2074.
7. Bustin SA, Nolan T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech 2004;15:155-166.
8. Edstrom E, Ulfhake B. Sarcopenia is not due to lack of regenerative drive in senescent skeletal muscle. Aging Cell. 2005;4:65-77.
9. Giresi PG, Stevenson EJ, Theilhaber J, Koncarevic A, Parkington J, Fielding RA, et al. Identification of a molecular signature of sarcopenia. Physiol Genomics 2005;21:253-263.
10. Jemiolo B, Trappe S. Single muscle fiber gene expression in human skeletal muscle: validation of internal control with exercise. Biochem Biophys Res Commun 2004;320:1043-1050.
11. Jonsdottir IH, Schjerling P, Ostrowski K, Asp S, Richter EA, Pedersen BK. Muscle contractions induce interleukin-6 mRNA production in rat skeletal muscles. J Physiol 2000;528 Pt 1:157-163.
12. Kimball SR, O'Malley JP, Anthony JC, Crozier SJ, Jefferson LS. Assessment of biomarkers of protein anabolism in skeletal muscle during the life span of the rat: sarcopenia despite elevated protein synthesis. Am J Physiol Endocrinol Metab 2004;287:E772-780.
13. Mahoney DJ, Carey K, Fu MH, Snow R, Cameron-Smith D, Parise G, et al. Real-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise. Physiol Genomics 2004;18:226-231.
14. Matsakas AA, Friedel A, Hertrampf T, Diel P. Short-term endurance training results in a muscle-specific decrease of myostatin mRNA content in the rat. Acta Physiol Scand 2005;183:299-307.
15. Whitman SA, Wacker MJ, Richmond SR, Godard MP. Contributions of the ubiquitin-proteasome pathway and apoptosis to human skeletal muscle wasting with age. Pflugers Arch 2005;450(6):437-446.
16. de Kok JB, Roelofs RW, Giesendorf BA, Pennings JL, Waas ET, Feuth T, et al. Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes. Lab Invest 2005;85:154-159.
17. Radonic AS, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun 2004;313:856-862.
18. Hsiao LL, Dangond F, Yoshida T, Hong R, Jensen RV, J. Misra, et al. A compendium of gene expression in normal human tissues. Physiol Genomics 2001;7:97-104.
19. Zhong H, Simons JW. Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem Biophys Res Commun 1999;259:523-526.
20. Murphy RM, Watt KK, Cameron-Smith D, Gibbons CJ, Snow RJ. Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR. Physiol Genomics 2003;12:163-174.
21. Schmittgen TD, Zakrajsek BA. Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods 2000;46:69-81.
22. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, et al. Housekeeping genes as internal standards: Use and limits. J Biotechnol 1999;75:291-295.
23. Bergstrom J. Muscle electrolytes in man. Scand J Clin Lab Invest. 1962;14:511-513.
24. Wacker MJ, Godard MP. Analysis of one-step and two-step real-time RT-PCR using SuperScript III. J Biomol Tech 2005;16(3):266-271.