A hypermorphic mutation in the protein phosphatase 2C HAB1 strongly affects ABA signaling in Arabidopsis

FEBS Letters

Volumn 580, Issue 19, 2006, Pages 4691-4696

  Robert, Nadia ^a   Merlot, Sylvain ^b   N'Guyen, Vinh ^a   Boisson Dernier, Aurélien ^a   Schroeder, Julian I ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b INSTITUT DES SCIENCES DU VÉGÉTAL   (France)

Author keywords

Abscisic acid; Dominant mutation; Hypermorphic; Protein phosphatase 2C

Indexed keywords

ABSCISIC ACID; ASPARTIC ACID; GLYCINE; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOPROTEIN PHOSPHATASE 2C; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; ENZYME ACTIVE SITE; EUKARYOTE; GENE; GENE MUTATION; PLANT STRESS; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS REGULATION; SIGNAL TRANSDUCTION;

ABSCISIC ACID; AMINO ACID SEQUENCE; ARABIDOPSIS; BASE SEQUENCE; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEIN PHOSPHATASE; POINT MUTATION; SEQUENCE HOMOLOGY, AMINO ACID; SIGNAL TRANSDUCTION;

ARABIDOPSIS; ARABIDOPSIS THALIANA; EUKARYOTA; PROKARYOTA;

EID: 33746909895     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.07.047     Document Type: Article

References (28)

1. Aubry L., and Firtel R.A. Spalten, a protein containing Gα-protein-like and PP2C domains, is essential for cell-type differentiation in Dictyostelium. Genes Dev. 12 (1998) 1525-1538
2. Voelker U., Dufour A., and Haldenwang W.G. The Bacillus subtilis rsbU gene product is necessary for RsbX-dependent regulation of sigma B. J. Bacteriol. 177 (1995) 114-122
3. Chin-Sang I.D., and Spence A.M. Caenorhabditis elegans sex-determining protein FEM-2 is a protein phosphatase that promotes male development and interacts directly with FEM-3. Genes Dev. 10 (1996) 2314-2325
4. Kerk D., Bulgrien J., Smith D.W., Barsam B., Veretnik S., and Gribskov M. The complement of protein phosphatase catalytic subunits encoded in the genome of Arabidopsis. Plant Physiol. 129 (2002) 908-925
5. Schweighofer A., Hirt H., and Meskiene I. Plant PP2C phosphatases: emerging functions in stress signaling. Trends Plant Sci. 9 (2004) 236-243
6. Koorneef M., Reuling G., and Karssen C.M. The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiol. Plant. 61 (1984) 377-383
7. Meyer K., Leube M.P., and Grill E. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science 264 (1994) 1452-1455
8. Leung J., Bouvier-Durand M., Morris P.C., Guerrier D., Chefdor F., and Giraudat J. Arabidopsis ABA response gene ABI1: features of a calcium-modulated protein phosphatase. Science 264 (1994) 1448-1452
9. Rodriguez P.L., Benning G., and Grill E. ABI2, a second protein phosphatase 2C involved in abscisic acid signal transduction in Arabidopsis. FEBS Lett. 421 (1998) 185-190
10. Leung J., Merlot S., and Giraudat J. The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell 9 (1997) 759-771
11. Gosti F., Beaudoin N., Serizet C., Webb A.A.R., Vartanian N., and Giraudat J. ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11 (1999) 1897-1910
12. Merlot S., Gosti F., Guerrier D., Vavasseur A., and Giraudat J. The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J. 25 (2001) 295-303
13. Wu Y., Sanchez J.P., Lopez-Molina L., Himmelbach A., Grill E., and Chua N.H. The abi1-1 mutation blocks ABA signaling downstream of cADPR action. Plant J. 34 (2003) 307-315
14. Leonhardt N., Kwak J.M., Robert N., Waner D., Leonhardt G., and Schroeder J.I. Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant. Plant Cell 16 (2004) 596-615
15. Saez A., Apostolova N., Gonzalez-Guzman M., Gonsalez-Garcia M.P., Nicolas C., Lorenzo O., and Rodriguez P.L. Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling. Plant J. 37 (2004) 354-369
16. Wesley S.V., Helliwell C.A., Smith N.A., Wang M.B., Rouse D.T., Liu Q., Gooding P.S., Singh S.P., Abbott D., Stoutjesdijk P.A., Robinson S.P., Gleave A.P., Green A.G., and Waterhouse P.M. Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J. 27 (2001) 581-590
17. Waterhouse P.M., Wang M.B., and Lough T. Gene silencing as an adaptive defence against viruses. Nature 411 (2001) 834-842
18. Clough S.J., and Bent A.F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16 (1998) 735-743
19. Guan K.L., and Dixon J.E. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192 (1991) 262-267
20. MacKintosh C. In: Hardie D.G. (Ed). Protein Phosphorylation: A Practical Approach (1993), Oxford University Press, Oxford, UK 197-230
21. Leube M.P., Grill E., and Amrhein N. ABI1 of Arabidopsis is a protein serine/threonine phosphatase highly regulated by the proton and magnesium ion concentration. FEBS Lett. 424 (1998) 100-104
22. Merlot S., Mustilli A.C., Genty B., North H., Lefebvre V., Sotta B., Vavasseur A., and Giraudat J. Use of infrared thermal imaging to isolate Arabidopsis mutants defective in stomatal regulation. Plant J. 30 (2002) 601-609
23. Herskowitz I. Functional inactivation of genes by dominant negative mutations. Nature 329 (1987) 219-222
24. Kuhn J.M., Boisson-Dernier A., Dizon M.B., Maktabi M.H., and Schroeder J.I. The protein phosphatase AtPP2CA negatively regulates abscisic acid signal transduction in Arabidopsis, and effects of abh1 on AtPP2CA mRNA. Plant Physiol. 140 (2006) 127-139
25. Yoshida T., Nishimura N., Kitahata N., Kuromori T., Ito T., Asami T., Shinozaki K., and Hirayama T. ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs. Plant Physiol. 140 (2006) 115-126
26. Meskiene I., Baudouin E., Schweighofer A., Liwosz A., Jonak C., Rodriguez P.L., Jelinek H., and Hirt H. Stress-induced protein phosphatase 2C is a negative regulator of a mitogen-activated protein kinase. J. Biol. Chem. 278 (2003) 18945-18952
27. Das A.K., Helps N.R., Cohen P.T., and Barford D. Crystal structure of the protein serine/threonine phosphatase 2C at 2.0 A resolution. EMBO J. 15 (1996) 6798-6809
28. Sheen J. Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants. Proc. Natl. Acad. Sci. USA 95 (1998) 975-980