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Volumn 30, Issue 3, 2002, Pages 189-191

Understanding heterologous protein overproduction under the T7 promoter: A practical exercise

Author keywords

Bacteria; Gene overexpression; Heterologous protein production; High levels of protein production; T7 promoter

Indexed keywords

ARTICLE; BIOCHEMISTRY; EDUCATION PROGRAM; EDUCATIONAL MODEL; GENE EXPRESSION; GENE OVEREXPRESSION; GENE TECHNOLOGY; HETEROLOGOUS EXPRESSION; HUMAN; LABORATORY; MOLECULAR BIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROKARYOTE; STUDENT;

EID: 33746867983     PISSN: 14708175     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmb.2002.494030030066     Document Type: Article
Times cited : (3)

References (7)
  • 3
    • 0026648406 scopus 로고
    • DNA binding protein from mesophilic and thermophilic Bacilli: Cloning, overexpression and purification
    • P. M. Padas, K. S. Wilson, C. E. Vorgias (1992) DNA binding protein from mesophilic and thermophilic Bacilli: cloning, overexpression and purification, Gene 117, 39-44.
    • (1992) Gene , vol.117 , pp. 39-44
    • Padas, P.M.1    Wilson, K.S.2    Vorgias, C.E.3
  • 4
    • 0032168451 scopus 로고    scopus 로고
    • Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima
    • E. Christodoulou, C. E. Vorgias (1998) Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima, Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 1043-1045.
    • (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. , vol.54 , pp. 1043-1045
    • Christodoulou, E.1    Vorgias, C.E.2
  • 5
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • U. K. Laemmli (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 7
    • 0003492874 scopus 로고    scopus 로고
    • S. J. Higgins, B. D. Harnes, Eds. Oxford University Press, Oxford
    • S. J. Higgins, B. D. Harnes, Eds. (1999) Protein Expression: A Practical Approach. Oxford University Press, Oxford.
    • (1999) Protein Expression: A Practical Approach


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.