Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima

Acta Crystallographica Section D: Biological Crystallography

Volumn 54, Issue 5, 1998, Pages 1043-1045

  Christodoulou, Evangelos ^a,b   Vorgias, Constantinos E ^a,b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF ATHENS   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMMONIUM SULFATE; CRYSTAL; CRYSTALLIZATION; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

BACTERIA; BACTERIAL PROTEINS; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA-BINDING PROTEINS; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMOTOGA MARITIMA;

EID: 0032168451     PISSN: 09074449     EISSN: None     Source Type: Journal    
DOI: 10.1107/S0907444998000341     Document Type: Article

References (21)

1. Broyles, S. & Petlijohn, D. E. (1986). J. Mol. Biol. 187, 47-60.
2. Craigie, R., Ardndt-Jovin, D. & Mizuuchi, D. (1985). Proc. Natl Acad. Sci. USA, 82, 7570-7574.
3. Drlica, K. & Rouviere-Yaniv, J. (1987). Microbiol. Rev. 51, 301-319.
4. Flashner, Y. & Gralla, J. D. (1988). Cell, 54, 713-721.
5. Inone, H., Nojima, H. & Okayama, H. (1990). Gene, 96, 23-28.
6. Jancarick, J., & Kim, S.-H. (1991). J. Appl. Cryst. 24, 409-411.
7. Laemmli, U. K. (1970). Nature (London), 227, 680-685.
8. Matthews, B. W. (1968). J. Mol. Biol. 33, 491-497.
9. Mensa-Wilmot, K., Carroll, K. & McMacken, R. (1989). EMBO J. 8, 2393-2402.
10. Otwinowski, Z. (1993). DENZO: An oscillation data processing program for macromolecular crystallography. Yale University, New Haven, CT, USA.
11. Padas, M. P., Wilson, K. S. & Vorgias, C. E. (1992). Gene, 117, 39-44.
12. Pettijohn, D. E. (1988). J. Biol. Chem. 263, 12793-12796.
13. Rouvière-Yaniv, J. & Gros, F. (1975). Proc. Natl Acad. Sci. USA, 72, 3428-3432, 1975.
14. Rouvière-Yaniv, J., Yaniv, M & Germond, J. (1979). Cell, 17, 265-274.
15. Sambrook, J., Fritsch, E. F. & Manialis, T. (1989). Molecular Cloning. A Laboratory Manual, 2nd ed. New York: Cold Spring Harbor Laboratory Press.
16. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990). Methods Enzymol. 185, 60-89.
17. Tanaka, I., Appelt, K, Dijk, J., White, S. W. & Wilson, K. S. (1984). Nature (London), 310, 376-381.
18. Van Slifhout, R. G. & Hermes, C. (1995). Rev. Sci. Immun. 66, 1818-1820.
19. Vis, H., Mariani, M., Vorgias, C. E., Wilson, K. S., Kaptein, R. & Boelens, R. (1995). J. Mol. Biol. 254, 692-703.
20. White, S., Appell, K., Wilson, K. S. & Imamoto, J. (1989). Proteins Struct. Funct. Genet. 5, 281-288.
21. Wilson, K. S., Vorgias, C. E., Tanaka, I., White, S. W. & Kimura, M. (1990). Protein Eng. 4, 11-22.