A reverse transcription-nested PCR assay for HHV-6 mRNA early transcript detection after transplantation

Journal of Virological Methods

Volumn 134, Issue 1-2, 2006, Pages 41-47

  Pradeau, Karine ^a,b   Bordessoule, Dominique ^a   Szelag, Jean Christophe ^a   Rolle, Florence ^a   Ferrat, Pierre ^a   Meur, Yann Le ^a   Turlure, Pascal ^a   Denis, François ^a   Ranger Rogez, Sylvie ^a,b  

^a DUPUYTREN UNIVERSITY HOSPITAL   (France)

^b UNIVERSITY OF LIMOGES   (France)

Author keywords

HHV 6 reactivation; mRNA detection; RT PCR; Transplant recipients; U79 80 gene

Indexed keywords

VIRUS MESSENGER RNA; MESSENGER RNA; PRIMER DNA; VIRUS RNA;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; BONE MARROW; COLORIMETRY; CONTROLLED STUDY; DIARRHEA; FEMALE; GRAFT DYSFUNCTION; GRAFT REJECTION; HEART TRANSPLANTATION; HUMAN; HUMAN CELL; HUMAN HERPESVIRUS 6; HYBRIDIZATION; IMMUNE DEFICIENCY; KIDNEY TRANSPLANTATION; LEUKOPENIA; MAJOR CLINICAL STUDY; MALE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RASH; RECURRENT INFECTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA TRANSCRIPTION; TRANSPLANTATION; VIRUS CULTURE; VIRUS REACTIVATION; VIRUS TYPING; VIRUS VIRULENCE; ANIMAL; BONE MARROW TRANSPLANTATION; CELL LINE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MIDDLE AGED; PHYSIOLOGY; POSTOPERATIVE COMPLICATION; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUS ACTIVATION; VIRUS GENE; VIRUS INFECTION;

HUMAN HERPESVIRUS 6;

ADULT; AGED; ANIMALS; BONE MARROW TRANSPLANTATION; CELL LINE; DNA PRIMERS; FEMALE; GENES, VIRAL; HEART TRANSPLANTATION; HERPESVIRUS 6, HUMAN; HUMANS; KIDNEY TRANSPLANTATION; MALE; MIDDLE AGED; POSTOPERATIVE COMPLICATIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, VIRAL; ROSEOLOVIRUS INFECTIONS; SENSITIVITY AND SPECIFICITY; VIRUS ACTIVATION;

EID: 33746761043     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.11.015     Document Type: Article

References (38)

1. Ablashi D.V., Agut H., Berneman Z., Campadelli-Fiume G., Carrigan D.R., Ceccerini-Nelli L., Chandran B., Chou S., Collandre H., Cone R., Dambaugh T.R., Dewhurst S., DiLuca D., Foa′-Tomasi L., Fleckenstein B., Frenkel N., Gallo R., Gompels U.A., Hall C., Jones M., Lawrence G., Martin M.E., Montagnier L., Neipel F., Nicholas J., Pellett P.E., Razzaque A., Torrelli G., Thomson B.J., Salahuddin S.Z., Wyatt L., and Yamanishi K. Human herpesvirus-6 strain groups: a nomenclature. Arch. Virol. 129 (1993) 363-366
2. Acott P.D., Lee S.H., Bitter-Suermann H., Lawen J.G., and Crocker J.F. Infection concomitant with pediatric renal allograft rejection. Transplantation 62 (1996) 689-691
3. Amo K., Tanaka-Taya K., Inagi R., Miyagawa H., Miyoshi H., Okusu I., Sashihara J., Hara J., Nakayama M., Yamanishi K., and Okada S. Human herpesvirus 6B infection of the large intestine of patients with diarrhea. Clin. Infect. Dis. 36 (2003) 120-123
4. Andre-Garnier E., Robillard N., Costa-Mattioli M., Besse B., Billaudel S., and Imbert-Marcille B.M. A one-step RT-PCR and a flow cytometry method as two specific tools for direct evaluation of human herpesvirus-6 replication. J. Virol. Methods 108 (2003) 213-222
5. Bethge W., Beck R., Jahn G., Mundinger P., Kanz L., and Einsele H. Successful treatment of human herpesvirus-6 encephalitis after bone marrow transplantation. Bone Marrow Transplant. 24 (1999) 1245-1248
6. Braun D.K., Dominguez G., and Pellett P.E. Human herpesvirus 6. Clin. Microbiol. Rev. 10 (1997) 521-567
7. Caserta M.T., Mock D.J., and Dewhurst S. Human herpesvirus 6. Clin. Infect. Dis. 33 (2001) 829-833
8. Collot S., Petit B., Bordessoule D., Alain S., Touati M., Denis F., and Ranger-Rogez S. Real-time PCR for quantification of human herpesvirus 6 DNA from lymph nodes and saliva. J. Clin. Microbiol. 40 (2002) 2445-2451
9. Cone R.W., Hackman R.C., Huang M.L., Bowden R.A., Meyers J.D., Metcalf M., Zeh J., Ashley R., and Corey L. Human herpesvirus 6 in lung tissue from patients with pneumonitis after bone marrow transplantation. N. Engl. J. Med. 329 (1993) 156-161
10. Cone R.W., Huang M.L., Ashley R., and Corey L. Human herpesvirus 6 DNA in peripheral blood cells and saliva from immunocompetent individuals. J. Clin. Microbiol. 31 (1993) 1262-1267
11. De Bolle L., Naesens L., and De Clercq E. Update on human herpesvirus 6 biology, clinical features, and therapy. Clin. Microbiol. Rev. 18 (2005) 217-245
12. Dockrell D.H. Human herpesvirus 6: molecular biology and clinical features. J. Med. Microbiol. 52 (2003) 5-18
13. Dockrell D.H., and Paya C.V. Human herpesvirus-6 and -7 in transplantation. Rev. Med. Virol. 11 (2001) 23-36
14. Gautheret-Dejean A., Manichanh C., Thien-Ah-Koon F., Fillet A.M., Mangeney N., Vidaud M., Dhedin N., Vernant J.P., and Agut H. Development of a real-time polymerase chain reaction assay for the diagnosis of human herpesvirus-6 infection and application to bone marrow transplant patients. J. Virol. Methods 100 (2002) 27-35
15. Hoshino K., Nishi T., Adachi H., Ito H., Fukuda Y., Dohi K., and Kurata T. Human herpesvirus-6 infection in renal allografts: retrospective immunohistochemical study in Japanese recipients. Transpl. Int. 8 (1995) 169-173
16. Jacobs U., Ferber J., and Klehr H.U. Severe allograft dysfunction after OKT3-induced human herpes virus-6 reactivation. Transplant. Proc. 26 (1994) 3121
17. Kasolo F.C., Mpabalwani E., and Gompels U.A. Infection with AIDS-related herpesviruses in human immunodeficiency virus-negative infants and endemic childhood Kaposi's sarcoma in Africa. J. Gen. Virol. 78 Pt 4 (1997) 847-855
18. Kearns A.M., Turner A.J., Taylor C.E., George P.W., Freeman R., and Gennery A.R. LightCycler-based quantitative PCR for rapid detection of human herpesvirus 6 DNA in clinical material. J. Clin. Microbiol. 39 (2001) 3020-3021
19. Kondo K., Kondo T., Shimada K., Amo K., Miyagawa H., and Yamanishi K. Strong interaction between human herpesvirus 6 and peripheral blood monocytes/macrophages during acute infection. J. Med. Virol. 67 (2002) 364-369
20. MacLean H.J., and Douen A.G. Severe amnesia associated with human herpesvirus 6 encephalitis after bone marrow transplantation. Transplantation 73 (2002) 1086-1089
21. Maeda Y., Teshima T., Yamada M., Shinagawa K., Nakao S., Ohno Y., Kojima K., Hara M., Nagafuji K., Hayashi S., Fukuda S., Sawada H., Matsue K., Takenaka K., Ishimaru F., Ikeda K., Niiya K., and Harada M. Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation. Br. J. Haematol. 105 (1999) 295-302
22. Miyoshi I., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y., Shiraishi Y., and Akagi T. Type C virus-producing cell lines derived from an adult T cell leukemia. GANN Monogr. Cancer Res. 28 (1982) 219-228
23. Morris D.J., Littler E., Arrand J.R., Jordan D., Mallick N.P., and Johnson R.W. Human herpesvirus 6 infection in renal-transplant recipients. N. Engl. J. Med. 320 (1989) 1560-1561
24. Nitsche A., Muller C.W., Radonic A., Landt O., Ellerbrok H., Pauli G., and Siegert W. Human herpesvirus 6A DNA Is detected frequently in plasma but rarely in peripheral blood leukocytes of patients after bone marrow transplantation. J. Infect. Dis. 183 (2001) 130-133
25. Norton R.A., Caserta M.T., Hall C.B., Schnabel K., Hocknell P., and Dewhurst S. Detection of human herpesvirus 6 by reverse transcription-PCR. J. Clin. Microbiol. 37 (1999) 3672-3675
26. Okuno T., Higashi K., Shiraki K., Yamanishi K., Takahashi M., Kokado Y., Ishibashi M., Takahara S., Sonoda T., Tanaka K., et al. Human herpesvirus 6 infection in renal transplantation. Transplantation 49 (1990) 519-522
27. Pescovitz M.D., and Navarro M.T. Immunosuppressive therapy and post-transplantation diarrhea. Clin. Transplant. 15 Suppl. 4 (2001) 23-28
28. Rieux C., Gautheret-Dejean A., Challine-Lehmann D., Kirch C., Agut H., and Vernant J.P. Human herpesvirus-6 meningoencephalitis in a recipient of an unrelated allogeneic bone marrow transplantation. Transplantation 65 (1998) 1408-1411
29. Salahuddin S.Z., Ablashi D.V., Markham P.D., Josephs S.F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-Staal F., Kramarsky B., et al. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science 234 (1986) 596-601
30. Sashihara J., Tanaka-Taya K., Tanaka S., Amo K., Miyagawa H., Hosoi G., Taniguchi T., Fukui T., Kasuga N., Aono T., Sako M., Hara J., Yamanishi K., and Okada S. High incidence of human herpesvirus 6 infection with a high viral load in cord blood stem cell transplant recipients. Blood 100 (2002) 2005-2011
31. Secchiero P., Carrigan D.R., Asano Y., Benedetti L., Crowley R.W., Komaroff A.L., Gallo R.C., and Lusso P. Detection of human herpesvirus 6 in plasma of children with primary infection and immunosuppressed patients by polymerase chain reaction. J. Infect. Dis. 171 (1995) 273-280
32. Singh N., and Carrigan D.R. Human herpesvirus-6 in transplantation: an emerging pathogen. Ann. Intern. Med. 124 (1996) 1065-1071
33. Tanaka-Taya K., and Okada S. Human herpesvirus-6 and -7 infection and bone marrow transplantation (BMT). Nippon Rinsho 56 (1998) 208-212
34. Taniguchi T., Shimamoto T., Isegawa Y., Kondo K., and Yamanishi K. Structure of transcripts and proteins encoded by U79-80 of human herpesvirus 6 and its subcellular localization in infected cells. Virology 271 (2000) 307-320
35. Tokimasa S., Hara J., Osugi Y., Ohta H., Matsuda Y., Fujisaki H., Sawada A., Kim J.Y., Sashihara J., Amou K., Miyagawa H., Tanaka-Taya K., Yamanishi K., and Okada S. Ganciclovir is effective for prophylaxis and treatment of human herpesvirus-6 in allogeneic stem cell transplantation. Bone Marrow Transplant. 29 (2002) 595-598
36. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., and Kurata T. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet 1 (1988) 1065-1067
37. Yoshikawa T., Akimoto S., Nishimura N., Ozaki T., Ihira M., Ohashi M., Morooka M., Suga S., Asano Y., Takemoto M., and Nishiyama Y. Evaluation of active human herpesvirus 6 infection by reverse transcription-PCR. J. Med. Virol. 70 (2003) 267-272
38. Yoshikawa T., Ihira M., Ohashi M., Suga S., Asano Y., Miyazaki H., Hirano M., Suzuki K., Matsunaga K., Horibe K., Kojima S., Kudo K., Kato K., Matsuyama T., and Nishiyama Y. Correlation between HHV-6 infection and skin rash after allogeneic bone marrow transplantation. Bone Marrow Transplant. 28 (2001) 77-81