Expression of the active human and duck hepatitis B virus polymerases in heterologous system of Pichia methanolica

Antiviral Research

Volumn 55, Issue 2, 2002, Pages 279-290

  Choi, Juhyun ^a,b   Kim, Eunice E ^c   Park, Young In ^b   Han, Ye Sun ^a  

^a Korea Institute of Science and Technology   (South Korea)

^b Korea University   (South Korea)

^c LG Chemical Ltd   (South Korea)

Author keywords

DNA dependent DNA polymerase; Hepatitis B virus polymerase; Pichia methanolic; RNA dependent DNA polymerase; RNase H

Indexed keywords

AMINO ACID; DNA DIRECTED DNA POLYMERASE; GLYCINE CLEAVAGE SYSTEM; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE; VIRUS ENZYME;

ARTICLE; CONTROLLED STUDY; DUCK; ENZYME ACTIVITY; ENZYME STABILITY; GENE EXPRESSION SYSTEM; HEPATITIS B VIRUS; NONHUMAN; PICHIA; PICHIA METHANOLICA; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION;

ANIMALS; CLONING, MOLECULAR; DNA-DIRECTED DNA POLYMERASE; DNA-DIRECTED RNA POLYMERASES; DUCKS; GENE PRODUCTS, POL; GENES, VIRAL; GENETIC VECTORS; HEPATITIS B VIRUS; HEPATITIS B VIRUS, DUCK; HUMANS; PICHIA; PLASMIDS; RECOMBINANT FUSION PROTEINS; RIBONUCLEASE H, CALF THYMUS; TEMPLATES, GENETIC;

ANAS SP.; DUCK HEPATITIS B VIRUS; HEPATITIS B VIRUS; PICHIA; PICHIA METHANOLICA;

EID: 0036289220     PISSN: 01663542     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-3542(02)00023-2     Document Type: Article

References (18)

1. Hepatitis B immune globulin (HBIG) efficacy in the interruption of perinatal transmission of hepatitis B virus carrier state. Initial report of a randomised double-blind placebo-controlled trial
2. Reconstitution of a functional duck hepatitis B virus replication initiation complex from separate reverse transcriptase domains expressed in Escherichia coli
3. Chromosome dynamics: Yeast pulls it apart
4. Binding properties of avian myeloblastosis virus DNA polymerases to nucleic acid affinity columns
5. Duck hepatitis B virus polymerase produced by in vitro transcription and translation possesses DNA polymerase and reverse transcriptase activities
6. The catalytic properties of human hepatitis B virus polymerase
7. Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase
8. Duck hepatitis B virus: DNA polymerase and reverse transcriptase activities of replicative complexes isolated from liver and their inhibition in vitro
9. Human hepatitis b virus polymerase interacts with the molecular chaperonin hsp60
10. Purification of a recombinant protein expressed in yeast: Optimization of analytical and preparative chromatography
11. Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1
12. Release of the hepatitis B virus-associated DNA polymerase from the viral particle by the proteolytic cleavage
13. Indentification of an epitope on the P and V proteins of Simian virus 5 that distinguishes between two isolates with different biological characteristics
14. Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate
15. Expression of functional hepatitis B virus polymerase in yeast reveals it to be the sole viral protein required for correct initiation of reverse transcription
16. The reverse transcriptase of hepatitis B virus acts as a protein primer for viral DNA synthesis
17. Therapy of chronic hepatitis B virus infection: Inhibition of the viral polymerase and other antiviral strategies