Expression and purification of exendin-4 dimer in Escherichia coli and its interaction with GLP-1 receptor in vitro

Protein and Peptide Letters

Volumn 13, Issue 8, 2006, Pages 823-827

  Yi, Lina ^a   Yin, Xiaopu ^a   Wei, Dongzhi ^a   Ma, Yushu ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Chemical cross linking; Exendin 4 dimer; Purification; Recombinant expression

Indexed keywords

EXENDIN 4; GLUCAGON LIKE PEPTIDE RECEPTOR; GLUCAGON RECEPTOR; GLUCAGON-LIKE PEPTIDE RECEPTOR; HYBRID PROTEIN; PEPTIDE; VENOM;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BINDING COMPETITION; CHEMISTRY; DIMERIZATION; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING;

AMINO ACID SEQUENCE; ANIMALS; BINDING, COMPETITIVE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIMERIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN BINDING; RECEPTORS, GLUCAGON; RECOMBINANT FUSION PROTEINS; VENOMS;

ESCHERICHIA COLI; HELODERMA SUSPECTUM;

EID: 33746736173     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986606777841226     Document Type: Article

References (29)

1. Bell, G.I., Sanchez-Pescador, R., Laybourn, P.J. and Najarian, R.C. (1983) Nature, 304, 368-371.
2. Nauck, M.A., Kleine, N., Orskov, C., Holst, J.J., Williams, B. and Creutzfeldt W. (1993) Diabetologia, 36, 741-744.
3. Gutniak, M., Orskov, C., Holst, J.J., Ahren, B. and Efendiæ, S. (1992) N. Engl. J. Med., 326, 1316-1322.
4. Meneilly, G.S., McIntosh, C.H., Pederson, R.A., Habener, J.F., Gingerich, R., Egan, J.M., Finegood, D.T. and Elahi, D. (2001) Diabetes Care, 24, 1951-1956.
5. Eng, J., Kleinman, W.A., Singh, L., Singh, G. and Raufman, J.P. (1992). J. Biol. Chem., 267, 7402-7405.
6. Meier, J.J., Nauck, M.A., Kranz, D., Holst, J.J., Deacon, C.F., Gaeckler, D., Schmidt, W.E. and Gallwitz, B. (2004) Diabetes, 53, 654-662.
7. Eng, J., Kleinman, W.A., Singh, L., Singh, G. and Raufman, J.P. (1992) Biol. Chem., 267, 7402-7405.
8. Drucker, D.J. (1998) Diabetes, 47, 159-169.
9. Tourrel, C., Bailbe, D., Meile, M.J., Kergoat, M. and Portha, B. (2001) Diabetes, 50, 1562-1569.
10. Kolterman, O.G., Buse, J.B., Fineman, M.S., Gaines, E., Heintz, S., Bicsak, T.A., Taylor, K., Kim, D., Aisporna, M., Wang, Y., Baron, A.D. (2003) J. Clin. Endocrinol. Metab., 88, 3082-3089.
11. De Leon, D.D., Deng, S., Madani, R., Ahima, R.S., Drucker, D.J. and Stoffers, D.A. (2003) Diabetes, 52, 365-371.
12. Szayna, M., Doyle, M.E., Betkey, J.A., Holloway, H.W., Spencer, R.G., Greig, N.H. and Egan, J.M. (2000) Endocrinology, 141, 1936-1941.
13. Edwards, C.M., Stanley, S.A., Davis, R., Brynes, A.E., Frost, G.S., Seal, L.J., Ghatei, M.A. and Bloom, S.R. (2001) Am. J. Physiol. Endocrinol. Metab., 281, E155-E161.
14. Nielsen, L.L., Young, A.A. and Parkes, D.G. (2004) Regul. Pept. , 117, 77-88.
15. Parkes, D., Jodka, C., Smith, P., Nayak, S., Rinehart, L., Gingerich, R., Chen, K. and Young, A. (2001) Drug Dev. Res., 53, 260-267.
16. Greig, N.H., Holloway, H.W., De Ore, K.A., Jani, D., Wang, Y., Zhou, J., Garant, M.J. and Egan, J.M. (1999) Diabetologia, 42, 45-50.
17. Navorro, M., Rodriquez, de Fonseca, F., Alvarez, E., Chowen, J.A., Zueco, J.A., Gomez, R., Eng, J. and Blazquez, E. (1996) J. Neurochem., 67, 1982-1991.
18. Young, A.A., Gedulin, B.R., Bhavsar, S., Bodkin, N., Jodka, C., Hansen, B. and Denaro, M. (1999) Diabetes, 48, 1026-1034.
19. Hupe-Sodmann, K., McGregor, G.P., Bridenbaugh, R., Goke, R., Goke, B., Thole, H., Zimmermann, B., Voigt, K. (1995) Regul. Pept., 58, 149-156.
20. Neidigh, J.W., Fesinmeyer, R.M., Prickett, K.S. and Andersen, N.H. (2001) Biochemistry, 40, 13188-13200.
21. Doyle, M.E., Theodorakis, M.J., Holloway, H.W., Bernier, M., Greig, N.H. and Egan, J.M. (2003) Regul. Pept., 114, 153-158.
22. Montrose-Rafizadeh, C., Yang, H., Rodgers, B.D., Beday, A., Pritchette, L.A. and Eng, J. (1997) Biol. Chem., 272, 21201-21206.
23. Deacon, C.F., Pridal, L., Klarskov, L., Olesen, M. and Holst, J.J. (1996) Am. J. Physiol. Endocrinol. Metab., 271, E458-E464.
24. Kieffer, T.J., McIntosh, C.H. and Pederson, R.A. (1995) Endocrinology, 136, 3585-3596.
25. Pridal, L., Deacon, C.F., Kirk, O., Christensen, J.V., Carr, R.D. and Holst, J.J. (1996) Eur. J. Drug Metab. Pharmacokinet., 21, 51-59.
26. Yin, X., Wei, D., Yi, L., Tao, X. and Ma, Y. (2005) Protein Expr. Purif., 41, 259-265.
27. Sinz, A. (2003) J. Mass Spectrom., 38, 1225-1237.
28. Schagger, H. and von Jagow, G. (1987) Anal. Biochem., 166, 368-379.
29. Badaut, C., Williams, R., Arluison, V., Bouffartigues, E., Robert, B., Buc, H. and Rimsky, S. (2002) J. Biol. Chem., 277, 41657-41666.