The Partec CyFlow Counter® could provide an option for CD4+ T-cell monitoring in the context of scaling-up antiretroviral treatment at the district level in Malawi

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 100, Issue 10, 2006, Pages 980-985

  Fryland, M ^a   Chaillet, P ^b   Zachariah, R ^b   Barnaba, A ^c   Bonte, L ^d   Andereassen, R ^a   Charrondiere S ^a   Teck, R ^a   Didakus, O ^a  

^a MÉDECINS SANS FRONTIÈRES   (Switzerland)

^b MÉDECINS SANS FRONTIÈRES   (Belgium)

^c MINISTRY OF HEALTH   (Malawi)

^d Medical Department   (France)

Author keywords

Antiretroviral therapy; ART; CD4; CyFlow Counter ; HIV; Malawi

Indexed keywords

ANTIRETROVIRUS AGENT;

ADOLESCENT; ADULT; AGED; ARTICLE; CD4+ T LYMPHOCYTE; CHILD; CONFIDENCE INTERVAL; CONTROLLED STUDY; CORRELATION COEFFICIENT; FEMALE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTERMETHOD COMPARISON; LYMPHOCYTE COUNT; MAJOR CLINICAL STUDY; MALAWI; MALE; PATIENT MONITORING; PUBLIC HOSPITAL; RELIABILITY; RURAL AREA; SCALE UP;

ANTIRETROVIRAL THERAPY, HIGHLY ACTIVE; CD4 LYMPHOCYTE COUNT; CD4-POSITIVE T-LYMPHOCYTES; FLOW CYTOMETRY; HIV INFECTIONS; HOSPITALS, DISTRICT; HUMANS; RURAL HEALTH; SENSITIVITY AND SPECIFICITY;

EID: 33746672648     PISSN: 00359203     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.trstmh.2005.11.014     Document Type: Article

References (23)

1. Bland J.M., and Altman D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1 (1986) 307-310
2. Cassens U., Gohde W., Kuling G., Groning A., Schlenke P., Lehman L.G., Traore Y., Servais J., Henin Y., Reichelt D., and Greve B. Simplified volumetric flow cytometry allows feasible and accurate determination of CD4 T lymphocytes in immunodeficient patients worldwide. Antivir. Ther. 9 (2004) 395-405
3. CDC. 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). Centers for Disease Control and Prevention. MMWR Recomm. Rep. 46 (1997) 1-29
4. Diagbouga S., Chazallon C., Kazatchkine M.D., Van de Perre P., Inwoley A., M'Boup S., David M.P., Tenin A.T., Soudre R., Aboulker J.P., and Weiss L. Successful implementation of a low-cost method for enumerating CD4 T lymphocytes in resource-limited settings: the ANRS 12-26 study. AIDS 17 (2003) 2201-2208
5. Didier J.M., Kazatchkine M.D., Demouchy C., Moat C., Diagbouga S., Sepulveda C., Di Lonardo A.M., and Weiss L. Comparative assessment of five alternative methods for CD4+ T-lymphocyte enumeration for implementation in developing countries. J. Acquir. Immune Defic. Syndr. 26 (2001) 193-195
6. Dieye T.N., Vereecken C., Diallo A.A., Ondoa P., Diaw P.A., Camara M., Karam F., Mboup S., and Kestens L. Absolute CD4 T-cell counting in resource-poor settings: direct volumetric measurements versus bead-based clinical flow cytometry instruments. J. Acquir. Immune Defic. Syndr. 39 (2005) 32-37
7. Greve B., Cassens U., Westerberg C., Ghde W., Sibrowski W., and Reichelt D. A new no-lyse, no wash flow-cytometric method for the determination of CD4 T cells in blood samples. Transfus. Med. Hemother. 30 (2003) 8-13
8. Janossy G., Jani I., and Gohde W. Affordable CD4(+) T-cell counts on 'single-platform' flow cytometers I. Primary CD4 gating. Br. J. Haematol. 111 (2000) 1198-1208
9. Kober K., and Van Damme W. Scaling up access to antiretroviral treatment in southern Africa: who will do the job?. Lancet 364 (2004) 103-107
10. Landay A., Ohlsson-Wilhelm B., and Giorgi J.V. Application of flow cytometry to the study of HIV infection. AIDS 4 (1990) 479-497
11. Lopez A., Caragol I., Candeias J., Villamor N., Echaniz P., Ortuno F., Sempere A., Strauss K., and Orfao A. Enumeration of CD4(+) T-cells in the peripheral blood of HIV-infected patients: an interlaboratory study of the FACSCount system. Cytometry 38 (1999) 231-237
12. Lyamuya E.F., Kagoma C., Mbena E.C., Urassa W.K., Pallagyo K., Mhalu F.S., and Biberfeld G. Evaluation of the FACScount, TRAx CD4 and Dynabeads methods for CD4 lymphocyte determination. J. Immunol. Methods 195 (1996) 103-112
13. Mandy F., Nicholson J., Autran B., and Janossy G. T-cell subset counting and the fight against AIDS: reflections over a 20-year struggle. Cytometry 50 (2002) 39-45
14. MOHP, 2003a. National AIDS Commission of Malawi. National estimate of HIV/AIDS in Malawi. Ministry of Health and Population, Lilongwe, Malawi.
15. MOHP, 2003b. Treatment of AIDS. The two year plan to scale up ART in Malawi (2004-2005). Ministry of Health and Population, Lilongwe, Malawi.
16. MOHP, 2003c. The status of human capacity development for an effective response to the HIV/AIDS epidemic in Malawi (evaluation report). Ministry of Health and Population, Lilongwe, Malawi.
17. MOHP, 2003d. Guidelines for the use of anti-retroviral therapy in Malawi, second ed. Ministry of Health and Population, Lilongwe, Malawi.
18. Mwaba P., Cassol S., Pilon R., Chintu C., Janes M., Nunn A., and Zumla A. Use of dried whole blood spots to measure CD4+ lymphocyte counts in HIV-1-infected patients. Lancet 362 (2003) 1459-1460
19. Saravolatz L., Neaton J.D., Sacks L., Deyton L., Rhame F., and Sherer R. CD4+ T lymphocyte counts and patterns of mortality among patients infected with human immunodeficiency virus who were enrolled in community programs for clinical research on AIDS. Clin. Infect. Dis. 22 (1996) 513-520
20. + T-cell enumeration in HIV infection with limited resources. J. Immunol. Methods 222 (1999) 209-217
21. WHO, 2003. Scaling up anti-retroviral therapy in resource-limited settings. Treatment guidelines for a public health approach. World Health Organization, Geneva, WHO, QV268.5 (revised version).
22. WHO, 2004. Rapid HIV tests. Guidelines for use in HIV testing and counselling services in resource-constrained settings. World Health Organization, Geneva.
23. + cell count determinations in Thailand. Clin. Diagn. Lab. Immunol. 4 (1997) 783-786