T-cell subset counting and the fight against AIDS: Reflections over a 20-year struggle

Clinical Cytometry

Volumn 50, Issue 2, 2002, Pages 39-45

  Mandy, Francis ^a   Nicholson, Jan ^b   Autran, Brigitte ^c   Janossy, George ^d  

^a HEALTH CANADA   (Canada)

^b NATL CENTER FOR INFECTIOUS DISEASES   (United States)

^c INSERM   (France)

^d ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

Author keywords

CD4 T lymphocytes; Flow cytometry; History of medicine; HIV; Immunophenotyping gating strategies; Naive memory T cells

Indexed keywords

ACCURACY; ACQUIRED IMMUNE DEFICIENCY SYNDROME; ARTICLE; BLOOD SAMPLING; CD4+ T LYMPHOCYTE; COST EFFECTIVENESS ANALYSIS; EPIDEMIC; FLOW CYTOMETRY; HISTORY OF MEDICINE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOPHENOTYPING; LABORATORY TEST; LEUKOCYTE; LYMPHOCYTE COUNT; MORPHOLOGY; PRACTICE GUIDELINE; PRIORITY JOURNAL; PUBLICATION; RELIABILITY; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; BLOOD; CLINICAL CHEMISTRY; CYTOLOGY; ECONOMICS; METHODOLOGY; REVIEW; ANTIGEN ANTIBODY COMPLEX; CD4 LYMPHOCYTE COUNT; CD8+ T LYMPHOCYTE; CELL INTERACTION; CELL REGENERATION; DIAGNOSTIC PROCEDURE; IMMUNE STATUS; MEMORY T LYMPHOCYTE; MONITORING; THYMUS;

CD4 ANTIGEN; MONOCLONAL ANTIBODY; ANTIRETROVIRUS AGENT; CD45 ANTIGEN; CD45RA ANTIGEN; L SELECTIN; PADGEM PROTEIN; PROTEINASE INHIBITOR; RNA DIRECTED DNA POLYMERASE INHIBITOR;

ACQUIRED IMMUNODEFICIENCY SYNDROME; ANTIBODIES, MONOCLONAL; ANTIGENS, CD4; CD4-POSITIVE T-LYMPHOCYTES; CHEMISTRY, CLINICAL; FLOW CYTOMETRY; HUMANS; IMMUNOPHENOTYPING; T-LYMPHOCYTE SUBSETS;

HUMAN IMMUNODEFICIENCY VIRUS;

EID: 0037092181     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.10097     Document Type: Article

References (62)

1. Marti-Ibanez F. Ariel essays on the arts and history and philosophy of medicine. New York: MD Publications; 1992. p. 179.
2. Giorgi JV, Detels R. T-cell subset alterations in HIV-infected homosexual men: NIAID multicenter AIDS cohort study. Clin Immunol Immunopathol 1989;52:10-18.
3. Fulwyler MJ. Flow cytometry and cell sorting. Blood Cells 1980;6: 173-184.
4. Coons AH, Creech HJ, Jones RN. Immunological properties of an antibody containing a fluorescent group. Proc Soc Exp Biol Med 1941;47:200.
5. Creech JH, Jones RN. Fluorescence protein labelling: an early Canadian contribution to HIV virus analysis and AIDS diagnosis. Can J Appl Spectrosc 1994;39:96-100.
6. Gottlieb MS, Schroff R, Schanker HM, Weisman JD, Fan PT, Wolf RA, Saxon A. Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency. N Engl J Med 1981;305:1425-1431.
7. Reinherz EL, Cooper MD, Schlossman SF, Rosen FS. Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders. J Clin Invest 1981;68:699-705.
8. Janossy G, Greaves MF. Lymphocyte activation. II. Discriminating stimulation of lymphocyte subpopulations by phytomitogens and heterologous antilymphocyte sera. Clin Exp Immunol 1972;10:525-536.
9. Keightley RG, Cooper MD, Lawton AR. The T cell dependence of B cell differentiation induced by pokeweed mitogen. J Immunol 1976; 117:1538-1544.
10. Janossy G, Gomez de la Concha E, Luquetti A, Snajdr MJ, Waxdal MJ, Platts-Mills TA. T-cell regulation of immunoglobulin synthesis and proliferation in pokeweed (Pa-1)stimulated human lymphocyte cultures. Scand J Immunol 1977;6:109-123.
11. Evans RL, Breard JM, Lazarus H, Schlossman SF, Chess L. Detection, isolation, and functional characterization of two human T-cell subclasses bearing unique differentiation antigens. J Exp Med 1977;145: 221-233.
12. Evans RL, Lazarus H, Penta AC, Schlossman SF. Two functionally distinct subpopulations of human T cells that collaborate in the generation of cytotoxic cells responsible for cell-mediated lympholysis. J Immunol 1978;120:1423-1428.
13. Tidman N, Janossy G, Bodger M, Granger S, Kung PC, Goldstein G. Delineation of human thymocyte differentiation pathways utilizing double-staining techniques with monoclonal antibodies. Clin Exp Immunol 1981;45:457-467.
14. White RA, Mason DW, Williams AF, Galfre G, Milstein C. T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med 1978;148:664-673.
15. Reinherz EL, Kung PC, Goldstein G, Schlossman SF. Further characterization of the human inducer T cell subset defined by monoclonal antibody. J Immunol 1979;123:2894-2896.
16. Reinherz EL, Kung PC, Goldstein G, Schlossman SF. A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol 1980;124:1301-1307.
17. Engleman EG, Benike CJ, Grumet FC, Evans RL. Activation of human T lymphocyte subsets: helper and suppressor/cytotoxic T cells recognize and respond to distinct histocompatibility antigens. J Immunol 1981;127:2124-2129.
18. Janossy G, Tidman N, Selby WS, Thomas JA, Granger S, Kung PC, Goldstein G. Human T lymphocytes of inducer and suppressor type occupy different microenvironments. Nature 1980;288:81-84.
19. Selby WS, Janossy G, Goldstein G, Jewell DP. T lymphocyte subsets in human intestinal mucosa: the distribution and relationship to MHC-derived antigens. Clin Exp Immunol 1981;44:453-458.
20. Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF. Leucocyte typing. Berlin: Springer Verlag; 1984.
21. Klatzmann D, Champagne E, Chamaret S, Gruest J, Guetard D, Hercend T, Gluckman JC, Montagnier L. T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV. Nature 1984;312: 767-768.
22. Dalgleish AG, Beverley PC, Clapham PR, Crawford DH, Greaves MF, Weiss RA. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 1984;312:763-767.
23. Barre-Sinoussi F, Chermann JC, Rey F, Nugeyre MT, Chamaret S, Gruest J, Dauguet C, Axler-Blin C, Vezinet-Brun F, Rouzioux C, Rozenbaum W, Montagnier L. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science 1983;220:868-871.
24. Gallo RC, Salahuddin SZ, Popovic M, Shearer GM, Kaplan M, Haynes BF, Palker TJ, Redfield R, Oleske J, Safai B, et al. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science 1984;224:500-503.
25. McDougal JS, Mawle A, Cort SP, Nicholson JK, Cross GD, Scheppler-Campbell JA, Hicks D, Sligh J. Cellular tropism of the human retrovirus HTLV-III/LAV. I. Role of T cell activation and expression of the T4 antigen. J Immunol 1985;135:3151-3162.
26. Sattentau QJ, Arthos J, Deen K, Hanna N, Healey D, Beverley PC, Sweet R, Truneh A. Structural analysis of the human immunodeficiency virus-binding domain of CD4. Epitope mapping with site-directed mutants and anti-idiotypes. J Exp Med 1989;170:1319-1334.
27. Shapiro HM. Practical flow cytometry. New York: Wiley-Liss; 1995.
28. Brando B, Barnett, D, Janossy, G, Mandy, F, Autran, B, Rothe, G, Sommagura, E, Cozzi, M, D'Hautcourt J, Lenkei R, Schmitz G, Kunkl A, Chianese R, Papa S, Gratama J. Cytometric methods for assessing absolute numbers of cell subsets in blood. Cytometry 2000;40:1-15.
29. Bergeron M, Nicholson JK, Phaneuf S, Ding T, Soucy N, Badley A, Hawley-Foss NC, Mandy F. 2002. Selection of lymphocyte gating protocol has impact on the level of reliability of T-cell subsets in aging specimens. Cytometry 50:53-61.
30. Glencross DK, Scott LE, Jani IV, Barnett D, Janossy G. CD45 assisted PanLeucogating for accurate, affordable dual platform CD4+ T cell enumeration. Cytometry 50:69-77.
31. Whitby L, Goodfellow K, Storie I, Granger V, Sawle A, Reilly JT, Barnett D. Quality control of CD4+ T-lymphocyte enumeration: results from the last nine years of United Kingdom External Quality-Assurance Scheme for Immune Monitoring (1993-2001). Cytometry 50:102-110.
32. Schnizlein-Bick CT, Francis F, Mandy FF, O'Gorman MRG, Paxton H, Nicholson JKA, Hultin LE, Gelman RS, Wilkening CL, Livnat D. Use of CD45 gating in three and four-color flow cytometric immunophenotyping: guideline from the National Institute of Allergy and Infectious Diseases, Division of AIDS. Cytometry 50:46-52.
33. Shapiro HM. Practical flow cytometry. New York: Wiley-Liss; 1995. p. 29.
34. Mandy FF, Bergeron M, Recktenwald D, Izaguirre CA. A simultaneous three-color T cell subsets analysis with single laser flow cytometers using T cell gating protocol. Comparison with conventional two-color immunophenotyping method. J Immunol Methods 1992;156: 151-162.
35. Nicholson JKA, Hubbard M, Jones BM. Use of CD45 fluorescence and side scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry. Cytometry 1996; 26:16-21.
36. Loken MR, Brosman JM, Bach BA, Ault KA. Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry 1990;11:453-459.
37. Janossy G, Jani IV, Goehde W. Affordable CD4+ T cell counts on 'single-platform' flow cytometers I. Primary CD4 gating. Br J Haematol 2000;111:1198-1208.
38. O'Gorman MR, Nicholson JK. Adoption of single-platform technologies for enumeration of absolute T-lymphocyte subsets in peripheral blood. Clin Diagn Lab Immunol 2000;7:333-335.
39. Janossy G, Jani I, Kahan M, Barnett D, Mandy F, Shapiro H. Precise CD4 T cell counting using red diode laser excitation: for ticher, for poorer. Cytometry 50:78-85.
40. Janossy G, Jani IV, Bradley NJ, Pitfield T, Glencross DK. Affordable CD4+ T cell counts by flow cytometry III. CD45 gating for optimal volumetric analysis. Clin Lab Diag Immunol (in press).
41. Janossy G, Glencross DK, Jani IV, Barnett D, Brando B, Mandy F, Shapiro HW. How to decrease the prices of counting CD4 T cells by flow cytometry while increasing accuracy, efficiency and quality? GMHC and project inform meeting on Monitoring and Diagnostic Tools for the Management of Antiretroviral Therapy in Resource-Poor Settings, Bethesda 11-13, November 2001. www.virology-education-. com
42. Ehrlich P. Croonian lecture: on immunity with special reference to cell life. Proc R Soc Lond [Biol] 1900;66:424.
43. Shapiro HM. Practical flow cytometry. New York: Wiley-Liss; 1995. p. 115-125.
44. de Kruif P. Microbe hunters. Orlando: Harcourt Brace; 1926.
45. Bergeron M, Faucher S, Ding T, Phaneuf S, Mandy F. Evaluation of a universal template for single-platform absolute T-lymphocyte subset enumeration. Cytometry 50:62-68.
46. Collier AC, Coombs RW, Schoenfeld DA, Bassett RL, Timpone J, Baruch A, Facey JM, Whitacre C, McAuliffe VJ, Friedman HM, Merigan TC, Reichman RC, Hooper C, Corey L. Treatment of human immunodeficiency virus infection with saquinavir, zidovudine, and zalcitabine. N Engl J Med 1996;334:1011-1017.
47. Hammer SM, Squires KE, Hughes MD, Grimes JM, Demeter LM, Currier JS, Eron JJ, Feinberg JE, Balfour HH, Deyton LR, Chodakewitz JA, Fischl MA, and the ACTG 320 study team. A controlled trial of two nucleoside analogues plus indinavir in persons with human immunodeficiency virus infection and CD4 cell counts of 200 per cubic millimeter or less. N Engl J Med 1997;337:725-739.
48. Katzenstein DA, Hammer SM, Hugues MD, et al. The relation of virologic and immunologic markers to clinical outcomes after nucleoside therapy in HIV-infected adults with 200 to 500 CD4 cells per cubic millimeter. N Engl J Med 1996;335:1091-1098.
49. Gulick RM, Mellors JW, Havlir D, Eron JJ, Gonzalez C, McMahon D, et al. Treatment with indinavir, zidovudine, and lamivudine in adults with human immunodeficiency virus infection and prior antiretroviral therapy. N Engl J Med 1997;337:734-739.
50. Autran B, Carcelain G, Li TS, Blanc C, Mathez D, Tubiana R, Katlama C, Debre P, Leibowitch J. Positive effects of combined anti-retroviral therapy on CD4+ T cell homeostasis and function in advanced HIV disease. Science 1997;277:112-116.
51. Pakker NG, Notermans DW, De Boer RJ, Roos MTL, De Wolf F, Hill A, Leonard JM, Danner SA, Miedema F, Schellekens PTA. Biphasic kinetics of peripheral blood T cells after triple combination therapy in HIV-1 infection: a composite of redistribution and proliferation. Nat Med 1998;2:208-214.
52. Lederman MM, Connick E, Landay A, Kuritzkes DR, Spritzler J, St Clair M, Kotzin BL, Fox L, Chiozzi MH, Leonard JM, Rousseau F, Wade M, Roe JD, Martinez A, Kessler H. Immunologic responses associated with 12 weeks of combination antiretroviral therapy consisting of Zidovudine, Lamivudine and Ritonavir: results of AIDS clinical trials group protocol 315. J Infect Dis 1998;178:70-79.
53. Connick E, Lederman MM, Kotzin BL, Spritzler J, Kuritzkes DR, St. Clair M, Fox L, Heath-Chiozzi M, Leonard JM, Rousseau F, Arc D, Roe J, Martinez A, Kessler H, Landay A. Immune reconstitution in the first year of potent antiretroviral therapy and its relationship to virologic response. J Infect Dis 2000;181:358-363.
54. Pontesilli O, Kerkhof-Garde S, Notermans DW, Foudraine NA, Roos MT, Klein MR, et al. Functional T cell reconstitution and human immunodeficiency virus-1-specific cell-mediated immunity during highly active antiretroviral therapy. J Infect Dis 1999;180:76-86.
55. Carcelain G, Blanc C, Leibowitch J, Mariot P, Mathez D, Schneider V, Saimot AG, Damont F, Simon F, Debre P, Autran B, Girard PM. T-cell changes after combined nucleoside analogue therapy in HIV primary infection. AIDS 1999;13:1077-1081.
56. Mackall CL, Granger L, Sheard MA, Cepeda R, Gress RE. T-cell regeneration after bone marrow transplantation: differential CD45 isoform expression on thymic-derived versus thymic-independent progeny. Blood 1993;82:2585-2894.
57. Mackall CL, Fleisher TA, Brown MR, Andrich MP, Chen CC, Feuerstein IM, et al. Age, thymopoiesis, and CD4+ T-lymphocyte regeneration after intensive chemotherapy. N Engl J Med 1995;332:143-149.
58. Bell ES, Sparshott SM. Interconversion of CD45R of CD4 T cells in vivo. Nature 1990;348:163-166.
59. Picker LJ, Treer JR, Ferguson DB, Collins PA, Buck D, Terstappen LW. Control of lymphocyte recirculation in man. I. Differential regulation of the peripheral lymph node homing receptor L-selection on T cells during the virgin to memory cell transition. J Immunol 1993;150: 1105-1121.
60. Douek DC, McFarlard RD, Keiser PH, Gage EA, Massey JM, Haynes BF, Polis MA, Haase AT, Feinberg MB, Sullivan JL, Jamieson BD, Zack JA, Picker LJ, Koup RA. Changes in thymic function with age and during the treatment of HIV infection. Nature 1998;396:690-695.
61. Poulin JF, Viswanathan MN, Harris JM, Komanduri KV, Wieder E, Ringuette N, Jenkins M, McCune JM, Sekaly RP. Direct evidence for thymic function in adult humans. J Exp Med 1999;190:479-486.
62. Zhang L, Lewin SR, Markowitz M, Lin HH, Skulsky E, Karanicolas R, He Y, Jin X, Tuttleton S, Vesanen M, Spiegel H, Kost R, van Lunzen J, Stellbrink HJ, Wolinsky S, Borkowsky W, Palumbo P, Kostrikis LG, Ho DD. Measuring recent thymic emigrants in blood of normal and HIV-1-infected individuals before and after effective therapy. J Exp Med 1999;190:725-732.