Production of polyclonal antibodies to a recombinant Potato mop-top virus non-structural triple gene block protein 1

Journal of Phytopathology

Volumn 154, Issue 7-8, 2006, Pages 422-427

  Cerovska N ^a   Filigarova M ^a   Pecenkova T ^a  

^a INSTITUTE OF EXPERIMENTAL BOTANY   (Czech Republic)

Author keywords

Polyclonal antibodies; Potato mop top virus; Recombinant protein; Triple gene block

Indexed keywords

ANTIBODY; GENE EXPRESSION; HOST PLANT; PLANT DEFENSE; PROTEIN; VIRUS;

BACTERIA (MICROORGANISMS); NICOTIANA BENTHAMIANA; ORYCTOLAGUS CUNICULUS; POTATO MOP-TOP VIRUS; SOLANUM TUBEROSUM;

EID: 33746340902     PISSN: 09311785     EISSN: 14390434     Source Type: Journal    
DOI: 10.1111/j.1439-0434.2006.01121.x     Document Type: Article

References (36)

1. Arif M, Torrance L, Reavy B. (1994) Improved efficiency of detection of potato mop-top furovirus in potato tubers and in the roots and leaves of soil-bait plants. Potato Res 37:373-381.
2. Avrameas S. (1969) Coupling of enzymes of proteins with glutaraldehyde. Use of the conjugate for the detection of antigens antibodies. Immunochemistry 6:32-52.
3. Čeřovská N, Moravec T, Rosecká P, Filigarová M, Pečenková T. (2003a) Nucleotide sequences of coat protein coding regions of six potato mop-top virus isolates. Acta Virol 47:41-44.
4. Čeřovská N, Moravec T, Rosecká P, Dédič P, Filigarová M. (2003b) Production of polyclonal antibodies to a recombinant coat protein of Potato mop-top virus. J Phytopathol 151:1-6.
5. Clark MF, Adams AN. (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475-483.
6. Cowan GH, Lioliopoulou F, Ziegler A, Torrance L. (2002) Subcellular localisation, protein interactions, and RNA binding of Potato mop-top virus triple gene block proteins. Virology 298:106-115.
7. Gasteiger E, Gattiker A, Hoogland C, Ivany I, Appel RD, Bairoch A. (2003) ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res 31:3784-3788.
8. Gorbalenya AE, Koonin EV. (1993) Helicases: amino acid sequence comparisons and structure-function relationships. Curr Opin Struct Biol 3:419-429.
9. Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM. (1989) Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res 26:4713-4730.
10. Helias V, Jacquot E, Guillet M, LeHingrat Y, Giblot-Ducray D. (2003) Production of recombinant Potato mop-top virus coat protein in Escherichia coli and generation of antisera recognising native virus protein. J Virol Methods 110:91-97.
11. Hirano H, Watanabe T. (1990) Microsequencing of proteins electrotransferred onto immobilizing matrices from polyacrylamide gel electrophoresis: application to an insoluble protein. Electrophoresis 11:573-580.
12. Jones RAC. Epidemiology and control of potato mop-top virus. In: Cooper JJ, Asher MJC (eds), Developments in Applied Biology: II. Viruses with Fungal Vectors, Wellesbourne, Association of Applied Biologists, 1988, pp. 255-270.
13. Kallender H, Buck KW, Brunt AA. (1990) Association of the three RNA molecules with potato mop-top virus. Neth J Plant Pathol 96:47-50.
14. Kashiwazaki S, Scott KP, Reavy B, Harrison BD. (1995) Sequence analysis and gene content of potato mop-top virus RNA 3: further evidence of heterogeneity in the genome organization of furoviruses. Virology 206:701-706.
15. Korimbocus J, Preston S, Danks C, Barker I, Coates D, Boonham N. (2002) Production of monoclonal antibodies to Sugarcane yellow leaf virus using recombinant readthrough protein. J Phytopathol 150:488-494.
16. Laemmli UK. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
17. Lin KS, Cheng SY. (1991) An efficient method to purify active eukaryotic proteins from inclusion bodies in Escherichia coli. Biotechnique 11:748-753.
18. Ling KS, Zhu HY, Jiang ZY, Gonsalves D. (2000) Effective application of DAS ELISA for detection of grapevine leafroll associated closterovirus-3 using a polyclonal antiserum developed from recombinant coat protein. Eur J Plant Pathol 106:301-309.
19. Mayo MA, Torrance L, Cowan G, Jolly CA, Macintosh SM, Orrega R, Barrera C, Salazar LF. (1996) Conservation of coat protein among isolates potato mop-top virus from Scotland and Peru. Arch Virol 141:1115-1121.
20. Morozov SI, Solovyev AG. (2003) Triple gene block: modular design of a multifunctional machine for plant virus movement. J Gen Virol 84(Pt 6): 1351-1366.
21. Mumford RA, Walsh K, Barker I, Boonham N. (2000) Detection of Potato mop-top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90:448-494.
22. Nielsen SL, Nicolaisen M. (2003) Identification of two nucleotide sequence sub-groups within Potato mop-top virus. Arch Virol 148:381-388.
23. Pečenková T, Moravec T, Filigarová M, Rosecká P, Čeřovská N. (2004) Extended sequence analysis of three Danish Potato mop-top virus (PMTV) isolates. Vir Genes 29:249-255.
24. Pečenková T, Filigarová M, Čeř ovská N. (2005) Efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein I modified by progressive deletion of its N-terminus. Protein Expr Purif 1:128-135.
25. Petrzik K, Mraz I, Kubelkova D. (2001) Preparation of recombinant coat protein of Prunus necrotic ringspot virus. Acta Virol 45:61-63.
26. Rantanen T, Lehtinen U, Kurppa A. (1999) Development of a IC-RT-PCR Test for Detection of Potato Mop-top Virus. Proceedings of the 14th Triennial Conference of the European Association for Potato Research, Sorento, Italy, p. 547-548.
27. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: a Laboratory Manual, 2nd edn, Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 1989.
28. Sandgren M, Savenkov EI, Valkonen JP. (2001) The readthrough region of Potato mop-top virus (PMTV) coat protein encoding RNA, the second largest RNA of PMTV genome, undergoes structural changes in naturally infected and experimentally inoculated plants. Arch Virol 146:467-477.
29. Savenkov EI, Sandgren M, Valkonen JP. (1999) Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus. J Gen Virol 80:2779-2784.
30. Savenkov EI, Germundsson A, Zamyatnin AA Jr, Sandgren M, Valkonen JPT. (2003) Potato mop-top virus: the coat protein-encoding RNA and the gene for cystein-rich protein are dispensable for systemic virus movement in Nicotiana benthamiana. J Gen Virol 84:1001-1005.
31. Solovyev GA, Savenkov EI, Grdzelishvili VZ, Kalinina NO, Morozov SY, Sciemann J, Atabekov JG. (1999) Movement of Hordei-virus hybrids with exchanges in the triple gene block. Virology 253:278-287.
32. Steinbuch M, Audran R. (1969) The isolation of IgG from mammalian sera with the aid of caprylic acid. Arch Biochem Biophys 34:279-284.
33. Thompson JD, Higgins DG, Gibson T. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680.
34. Torrance L, Mayo MA. (1997) Proposed re-classification of furoviruses. Arch Virol 142:435-439.
35. Vaira AM, Vecchiati M, Masenka V, Accotto GP. (1996) A polyclonal antiserum against recombinant viral protein combines specifity and versatility. J Virol Methods 56:209-219.
36. White RF, Kassanis B, James M. (1972) Potato mop-top virus in infected cells. J Gen Virol 15:175-177.