Efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein 1 modified by progressive deletion of its N-terminus

Protein Expression and Purification

Volumn 41, Issue 1, 2005, Pages 128-135

  Pečenková, Tamara ^a   Filigarová, Marie ^a   Čelovská, Noemi ^a  

^a INSTITUTE OF EXPERIMENTAL BOTANY   (Czech Republic)

Author keywords

Exonuclease III deletion; Potato mop top virus; Protein expression; Triple gene block

Indexed keywords

EID: 15744394885     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.12.013     Document Type: Article

References (32)

1. S. Ghoshroy, R. Lartey, J. Sheng, and V. Citovsky Transport of proteins and nucleic acids through plasmodesmata Annu. Rev. Plant Physiol. Plant Mol. Biol. 48 1997 27 49
2. M. Rouleau, R.J. Smith, J.B. Bancroft, and G.A. Mackie Purification, properties, and subcellular localization of foxtail mosaic potexvirus 26-kDa protein Virology 204 1994 254 265
3. S.Yu. Morozov, V.V. Dolja, and J.G. Atabekov Probable reassortment of genomic elements among elongated RNA-containing plant viruses J. Mol. Evol. 29 1989 52 62
4. L. Torrance, and M.A. Mayo Proposed reclassification of furoviruses Arch. Virol. 142 1991 435 439
5. R.A.C. Jones Epidemiology and control of potato mop-top virus J.J. Cooper M.J.C. Asher Developments in Applied Biology II. Viruses with Fungal Vectors 1988 Association of Applied Biologists Wellesbourne 255 270
6. R.F. White, B. Kassanis, and M. James Potato mop-top virus in infected cells J. Gen. Virol. 15 1972 175 177
7. H. Kallender, K.W. Buck, and A.A. Brunt Association of the three RNA molecules with potato mop-top virus Neth. J. Plant Pathol. 96 1990 47 50
8. E.I. Savenkov, M. Sandgren, and J.P. Valkonen Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus J. Gen. Virol. 80 1999 2779 2784
9. K.P. Scott, S. Kashiwazaki, B. Reavy, and B.D. Harrison The nucleotide sequence of potato mop-top virus RNA 2: a novel type of organization for a furovirus J. Gen. Virol. 75 1994 3561 3568
10. M.A. Mayo, L. Torrance, G. Cowan, C.A. Jolly, S.M. Macintosh, R. Orrega, C. Barrera, and L.F. Salazar Conservation of coat protein among isolates potato mop-top virus from Scotland and Peru Arch. Virol. 141 1996 1115 1121
11. M. Sandgren, E.I. Savenkov, and J.P. Valkonen The readthrough region of Potato mop-top virus (PMTV) coat protein encoding RNA, the second largest RNA of PMTV genome, undergoes structural changes in naturally infected and experimentally inoculated plants Arch. Virol. 146 2001 467 477
12. N. Čeřovská, T. Moravec, P. Rosecká, M. Filigarová, and T. Pečenková Nucleotide sequences of coat protein coding regions of six potato mop-top virus isolates Arch. Virol. 47 2003 41 44
13. S.L. Nielsen, and M. Nicolaisen Identification of two nucleotide sequence sub-groups within Potato mop-top virus Arch. Virol. 148 2003 381 388
14. T. Pečenková, T. Moravec, M. Filigarová, P. Rosecká, and N. Čeřovská Extended sequence analysis of three Danish Potato mop-top virus (PMTV) isolates Vir. Genes 29 2004 249 255
15. A. Kurppa Potato mop-top virus:purification, preparation of antisera and detection by means of ELISA Ann. Agric. Fenn. 28 1990 285 295
16. L. Torrance, G.H. Cowan, and L.G. Pereira Monoclonal antibodies specific for potato mop-top virus,and some properties of the coat protein Ann. Appl. Biol. 122 1993 311 322
17. S.L. Nielsen, and J.P. Molgaard Incidence, appearance and development of potato mop-top furovirus-induced spraing in potato cultivars and the influence on yield, distribution in Denmark and detection of the virus in tubers by ELISA Potato Res. 40 1997 101 110
18. N. Čeřovská, T. Moravec, P. Rosecká, P. Dědič, and M. Filigarová Production of polyclonal antibodies to a recombinant coat protein of Potato mop-top virus J. Phytopathol. 151 2003 1 6
19. V. Helias, E. Jacqout, M. Guillet, Y. Le Hingrat, and D. Giblot-Ducry Production of recombinant Potato mop-top virus coat protein in Escherichia coli and generation of antisera recognising native virus protein J. Virol. Methods 110 2003 91 97
20. A. Germundsson, M. Sandgren, H. Barker, E.I. Savenkov, and J.P.T. Valkonen Initial infection of roots and leaves reveals different resistance phenotypes associated with coat protein gene-mediated resistance to Potato mop-top virus J. Gen. Virol. 83 2002 1201 1209
21. E.I. Savenkov, A. Germundsson, A.A. Zamyatnin Jr., M. Sandgren, and J.P.T. Valkonen Potato mop-top virus: the coat protein-encoding RNA and the gene for cystein-rich protein are dispensable for systemic virus movement in Nicotiana benthamiana J. Gen. Virol. 84 2003 1001 1005
22. M. Arif, L. Torrance, and B. Reavy Improved efficiency of detection of potato mop-top furovirus in potato tubers and in the roots and leaves of soil-bait plants Potato Res. 37 1994 373 381
23. T. Rantanen, U. Lehtinen, A. Kurppa, Development of a IC-RT-PCR test for detection of potato mop-top virus, in: Proceedings of the 14th Triennial Conference of the European association for potato research, Sorento, Italy, 1999
24. R.A. Mumford, K. Walsh, I. Barker, and N. Boonham Detection of Potato mop-top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay Phytopathology 90 2000 448 494
25. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular cloning: A Laboratory Manual second ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor
26. E. Gasteiger, A. Gattiker, C. Hoogland, I. Ivanyi, R.D. Appel, and A. Bairoch ExPASY: the proteomics server for in-depth protein knowledge and analysis Nucleic Acids Res. 31 2003 3784 3788
27. J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res. 22 1994 4673 4680
28. B. Rost, G. Yachdav, and J. Liu The PredictProtein server Nucleic Acids Res. 32 2003 W321 W326
29. K.S. Lin, and S.Y. Cheng An efficient method to purify active eukaryotic proteins from inclusion bodies in Escherichia coli Biotechnique 11 1991 748 753
30. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature (London 227 1970 680 685
31. H. Hirano, and T. Watanabe Microsequencing of proteins electrotransferred onto immobilizing matrices from polyacrylamide gel electrophoresis: application to an insoluble protein Electrophoresis 11 1990 573 580
32. J. Liu, and B. Rost NORSp: prediction of long regions without regular structure Nucleic Acids res. 31 2003 3833 3835