Dynamic behavior of individual cells in developing organotypic brain slices revealed by the photoconvertable protein Kaede

Experimental Neurology

Volumn 200, Issue 2, 2006, Pages 430-437

  Mutoh, T ^a   Miyata, T ^a   Kashiwagi, S ^a   Miyawaki, A ^a   Ogawa, M ^a  

^a RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

Author keywords

3D time lapse imaging; Cell migration; Electroporation; Fluorescent protein; Kaede; Neocortical histogenesis; Neuronal progenitor; Organotypic slice culture; Photoconversion

Indexed keywords

KAEDE PROTEIN; PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CELL MIGRATION; CELL STRUCTURE; CONTROLLED STUDY; DAUGHTER CELL; EMBRYO; FLUORESCENCE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPINDLE CELL; STEM CELL; ULTRAVIOLET IRRADIATION;

ANIMALS; CELL COUNT; CELL DIFFERENTIATION; CELL MOVEMENT; CEREBRAL CORTEX; FEMALE; LUMINESCENT PROTEINS; MICE; MICE, INBRED ICR; NEURONS; ORGAN CULTURE TECHNIQUES; ORGANOGENESIS; PREGNANCY; STEM CELLS; TIME FACTORS; TRANSFECTION; ULTRAVIOLET RAYS;

EID: 33746312920     PISSN: 00144886     EISSN: 10902430     Source Type: Journal    
DOI: 10.1016/j.expneurol.2006.03.022     Document Type: Article

References (12)

1. Ando R., Hama H., Yamamoto-Hino M., Mizuno H., and Miyawaki A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 12651-12656
2. Mizuno H., Mal T., Tong K., Ando R., Furuta T., Ikura M., and Miyawaki A. Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein. Mol. Cell 4 (2003) 1051-1058
3. Matz M., Lukyanov K., and Lukyanov S. Family of the green fluorescent protein: journey to the end of the rainbow. BioEssays 24 (2002) 953-959
4. Miyata T., Kawaguchi A., Saito K., Kuramochi H., and Ogawa M. Visualization of cell cycling by an improvement in slice culture methods. J. Neurosci. Res. 69 (2002) 861-868
5. Miyata T., Kawaguchi A., Saito K., Kawano M., Mutoh T., and Ogawa M. Asymmetric production of surface-dividing and non-surfacedividing cortical progenitor cells. Development 131 (2004) 3133-3145
6. Miyawaki A. Fluorescent proteins in a new light. Nat. Biotechnol. 22 (2004) 1374-1376
7. Noctor S., Martinez-Cerdeno V., Ivic L., and Kriegstein A. Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases. Nat. Neurosci. 7 (2004) 136-144
8. Pinto-Lord MC., Evrard P., and Caviness Jr. V.S. Obstructed neuronal migration along radial glial fibers in the neocortex of the reeler mouse: a Golgi-EM analysis. Brain Res. 256 (1982) 379-393
9. Sanada K., Gupta A., and Tsai LH. Disabled-1-regulated adhesion of migrating neurons to radial glial fiber contributes to neuronal positioning during early corticogenesis. Neuron 42 (2004) 197-211
10. Tabata H., and Nakajima K. Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex. Neuroscience 103 (2001) 865-872
11. Tamamaki N., Sugimoto Y., Tanaka K., and Takauji R. Cell migration from the ganglionic eminence to the neocortex investigated by labeling nuclei with UV radiation via a fiber-optic cable. Neurosci. Res. 35 (1999) 241-251
12. Tutsui H., Karasawa S., Shimizu H., Nukina N., and Miyawaki A. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (2005) 233-238