DGGE-based detection method for Quahog Parasite Unknown (QPX)

Diseases of Aquatic Organisms

Volumn 70, Issue 1-2, 2006, Pages 115-122

  Gast, R J ^a   Cushman, E ^b   Moran, D M ^a   Uhlinger, K R ^c   Leavitt, D ^d   Smolowitz, R ^c  

^a WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

^b PALM BEACH ATLANTIC UNIVERSITY   (United States)

^c MARINE BIOLOGICAL LABORATORY   (United States)

^d ROGER WILLIAMS UNIVERSITY   (United States)

Author keywords

Detection limit; Quahog Parasite Unknown; Seed clams; SSU rDNA

Indexed keywords

DETECTION METHOD; ELECTROKINESIS; ENZYME; MORTALITY; PARASITIC DISEASE; SHELLFISH;

NORTH AMERICA; UNITED STATES;

BIVALVIA; MERCENARIA MERCENARIA;

EID: 33746059060     PISSN: 01775103     EISSN: None     Source Type: Journal    
DOI: 10.3354/dao070115     Document Type: Article

References (17)

1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
2. Austin B (1988) Quantification of marine microbial populations. Marine microbiology. Cambridge University Press, Cambridge, p 31-44
3. Brothers C, Marks E III, Smolowitz R (2000) Conditions affecting the growth and zoosporulation of the protistan parasite QPX in culture. Biol Bull (Woods Hole) 199:200-201
4. Cole JR, Chai B, Farris RJ, Wang Q, Kulam SA, McGarrell DM, Garrity GM, Tiedje JM (2005) The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 33:D294-D296
5. Drinnan RE, Henderson EB (1963) 1962 mortalities and possible disease organism in Neguac quahogs. Annual Report No. B11, Biological Station, St. Andrews, NB
6. Ford SE, Smolowitz R, Ragone Calvo LM, Barber RD, Kraeutner JN (1997) Evidence that QPX (quahog parasite unknown) is not present in hatchery-produced hard clam seed. J Shellfish Res 16:519-521
7. Gast RJ, Dennett MR, Caron DA (2004) Characterization of protistan assemblages in the Ross Sea, Antarctica by denaturing gradient gel electrophoresis. Appl Environ Microbiol 70:2028-2037
8. Kanagawa T (2003) Bias and artifacts in multitemplate polymerase chain reactions (PCR). J Biosci Bioeng 96:317-323
9. Kleinschuster SJ, Smolowitz R, Parent J (1998) In vitro life cycle and propagation of Quahog Parasite Unknown. J Shellfish Res 17:75-78
10. Lyons MM, Ward JE, Smolowitz R, Uhlinger KR, Gast RJ (2005) Lethal marine snow: pathogen of bivalve mollusc concealed in marine aggregates. Limnol Oceanogr 50:1983-1988
11. Maas PAY, Kleinshuster SJ, Dykstra MJ, Smolowitz R, Parent J (1999) Molecular characterization of QPX (Quahog Parasite Unknown), a pathogen of Mercenaria mercenaria. J Shellfish Res 18:561-567
12. Mo C, Douek J, Rinkevich B (2002) Development of a PCR strategy for thraustochytrid identification based on 18S rDNA sequence. Mar Biol 140:883-889
13. Smolowitz R, Leavitt D, Perkins F (1998) Observations of a protistan disease similar to QPX in Mercenaria mercenaria (hard clams) from the coast of Massachusetts. J Invertebr Pathol 71:9-25
14. Smolowitz R, Leavitt D, Lancaster B, Marks E, Hanselmann R, Brothers C (2001) Laboratory based transmission studies of quahog parasite unknown (QPX) in Mercenaria mercenaria. Aquaculture (book of abstracts) 2001:599
15. Spatz MJ, Anderson JL, Jancart S (1996) Northeast region aquaculture situation and outlook report. Report No. 3352, Northeastern Regional Aquaculture Center, Rhode Island Agricultural Experiment Station, Kingston, RI
16. Stokes NA, Ragone Calvo LM, Reece KS, Burreson EM (2002) Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria. Dis Aquat Org 52:233-247
17. Whyte SK, Cawthorn RJ, McGladdery SE (1994) QPX (Quahaug Parasite X) a pathogen of northern quahaug Mercenana mercenaria from the Gulf of St. Lawrence, Canada. Dis Aquat Org 19:129-136