Disruption of the Aspergillus fumigatus ECM33 homologue results in rapid conidial germination, antifungal resistance and hypervirulence

Microbiology

Volumn 152, Issue 7, 2006, Pages 1919-1928

  Romano, Jacob ^a   Nimrod, Guy ^a   Ben Tal, Nir ^a   Shadkchan, Yona ^a   Baruch, Koti ^a   Sharon, Haim ^a   Osherov, Nir ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOTERICIN B; ANTIFUNGAL AGENT; CAFFEINE; CASPOFUNGIN; CELL MEMBRANE PROTEIN; DODECYL SULFATE SODIUM; GLYCOSYLPHOSPHATIDYLINOSITOL; HYGROMYCIN B; ITRACONAZOLE; TRIFLUOPERAZINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBIOTIC RESISTANCE; ARTICLE; ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; CELL ADHESION; CELL MEMBRANE PERMEABILITY; CONIDIUM; CONTROLLED STUDY; DISEASE MODEL; FEMALE; FUNGAL CELL; FUNGAL SPORE GERMINATION; FUNGAL VIRULENCE; GENE DISRUPTION; IMMUNE DEFICIENCY; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR CLONING; MORPHOGENESIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FAMILY; PROTEIN FUNCTION; SEQUENCE HOMOLOGY; SURVIVAL TIME;

ANIMALS; ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; CELL WALL; CONGO RED; DRUG RESISTANCE, FUNGAL; FEMALE; FUNGAL PROTEINS; MICE; MICE, INBRED ICR; MORPHOGENESIS; PEPTIDES, CYCLIC; VIRULENCE;

ASPERGILLUS FUMIGATUS; FUNGI;

EID: 33746032587     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.28936-0     Document Type: Article

References (30)

1. Bainbridge, B. W. (1971). Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans. J Gen Microbiol 66, 319-325.
2. Berezin, C., Glaser, F., Rosenberg, J., Paz, I., Pupko, T., Fariselli, P., Casadio, R. & Ben-Tal, N. (2004). ConSeq: the identification of functionally and structurally important residues in protein sequences. Bioinformatics 20, 1322-1324.
3. Bernard, M. & Latgé, J.-P. (2001). Aspergillus fumigatus cell wall: composition and biosynthesis. Med Mycol 39, 9-17.
4. Bruneau, J. M., Magnin, T., Tagat, E., Legrand, R., Bernard, M., Diaquin, M., Fudali, C. & Latgé, J.-P. (2001). Proteome analysis of Aspergillus fumigatus identifies glycosylphosphatidylinositol-anchored proteins associated to the cell wall biosynthesis. Electrophoresis 22, 2812-2823.
5. Caro, L. H. P., Tettelin, H., Vossen, J. H., Ram, A. F. J., van den Ende, H. & Klis, F. M. (1997). In silicio identification of glycosyl-phosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae. Yeast 13, 1477-1489.
6. de Groot, P. W., Hellingwerf, K. J. & Klis, F. M. (2003). Genome-wide identification of fungal GPI proteins. Yeast 20, 781-796.
7. Eisenhaber, B., Schneider, G., Wildpaner, M. & Eisenhaber, F. (2004). A sensitive predictor for potential GPI lipid modification sites in fungal protein sequences and its application to genome-wide studies for Aspergillus nidulans, Candida albicans, Neurospora crassa, Saccharomyces cerevisiae and Schizosaccharomyces pombe. J Mol Biol 337, 243-253.
8. Greenstein, S., Shadkchan, Y., Jadoun, J., Sharon, C., Markovich, S. & Osherov, N. (2006). Analysis of the Aspergillus nidulans thaumatin-like cetA gene and evidence for transcriptional repression of pyr4 expression in the cetA-disrupted strain. Fungal Genet Biol 43, 42-53.
9. Jadoun, J., Shadkchan, Y. & Osherov, N. (2004). Disruption of the Aspergillus fumigatus argB gene using a novel in vitro transposon-based mutagenesis approach. Curr Genet 45, 235-241.
10. Jin, J., Lee, Y. K. & Wickes, B. L. (2004). Simple chemical extraction method for DNA isolation from Aspergillus fumigatus and other Aspergillus species. J Clin Microbiol 42, 4293-4296.
11. Kamran, M., Calcagno, A. M., Findon, H. & 9 other authors (2004). Inactivation of transcription factor gene ACE2 in the fungal pathogen Candida glabrata results in hypervirulence. Eukaryot Cell 3, 546-552.
12. Kartsonis, N. A., Nielsen, J. & Douglas, C. M. (2003). Caspofungin: the first in a new class of antifungal agents. Drug Resist Updat 6, 197-218.
13. Latgé, J.-P. (1999). Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev 12, 310-350.
14. Latgé, J.-P., Mouyna, I., Tekaia, F., Beauvais, A., Debeaupuis, J. P. & Nierman, W. (2005). Specific molecular features in the organization and biosynthesis of the cell wall of Aspergillus fumigatus. Med Mycol 43, S15-S22.
15. Martinez-Lopez, R., Monteoliva, L., Diez-Orejas, R., Nombela, C. & Gil, C. (2004). The GPI-anchored protein CaEcm33p is required for cell wall integrity, morphogenesis and virulence in Candida albicans. Microbiology 150, 3341-3354.
16. Martinez-Lopez, R., Park, H., Myers, C. L., Gil, C. & Filler, S. G. (2006). Candida albicans Ecm33p is important for normal cell wall architecture and interactions with host cells. Eukaryot Cell 5, 140-147.
17. May, G. S. & Morris, N. R. (1988). Developmental regulation of a conidiation-specific β-tubulin in Aspergillus nidulans. Dev Biol 128, 406-414.
18. Osherov, N., Kontoyiannis, D. P., Romans, A. & May, G. S. (2001). Resistance to itraconazole in Aspergillus nidulans and Aspergillus fumigatus is conferred by extra copies of the A. nidulans P-450 14-α demethylase gene, pdmA. J Antimicrob Chemother 48, 75-81.
19. Osherov, N., Mathew, J., Romans, A. & May, G. S. (2002). Identification of conidial-enriched transcripts in Aspergillus nidulans using suppression subtractive hybridization. Fungal Genet Biol 37, 197-204.
20. Pain, A., Woodward, J., Quail, M. A. & 22 other authors (2004). Insight into the genome of Aspergillus fumigatus: analysis of a 922 kb region encompassing the nitrate assimilation gene cluster. Fungal Genet Biol 41, 443-453.
21. Pardo, M., Monteoliva, L., Pla, J., Sánchez, M., Gil, C. & Nombela, C. (1999). Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall. Yeast 15, 459-472.
22. Pardo, M., Monteoliva, L., Vázquez, P., Martínez, R., Molero, G., Nombela, C. & Gil, C. (2004). PSTI and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity. Microbiology 150, 4157-4170.
23. Percival-Smith, A. & Segall, J. (1987). Increased copy number of the 5′ end of the SPS2 gene inhibits sporulation of Saccharomyces cerevisiae. Mol Cell Biol 7, 2484-2490.
24. Punt, P. J., Oliver, R. P., Dingemanse, M. A., Pouwels, P. H. & van den Hondel, C. A. (1987). Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56, 117-124.
25. Reinoso-Martin, C., Schuller, C., Schuetzer-Muehlbauer, M. & Kuchler, K. (2003). The yeast protein kinase C cell integrity pathway mediates tolerance to the antifungal drug caspofungin through activation of Slt2p mitogen-activated protein kinase signaling. Eukaryot Cell 2, 1200-1210.
26. Steinbach, W. J. & Stevens, D. A. (2003). Review of newer antifungal and immunomodulatory strategies for invasive aspergillosis. Clin Infect Dis 37, S157-S187.
27. Terashima, H., Hamada, K. & Kitada, K. (2003). The localization change of Ybr078w/Ecm33, a yeast GPI-associated protein, from the plasma membrane to the cell wall, affecting the cellular function. FEMS Microbiol Lett 218, 175-180.
28. Tougan, T., Chiba, Y., Kakihara, Y., Hirata, A. & Nojima, H. (2002). Meu10 is required for spore wall maturation in Schizosaccharomyces pombe. Genes Cells 7, 217-231.
29. Tsitsigiannis, D. I., Bok, J.-W., Andes, D., Nielsen, K. F., Frisvad, J. C. & Keller, N. P. (2005). Aspergillus cyclooxygenase-like enzymes are associated with prostaglandin production and virulence. Infect Immun 73, 4548-4559.
30. Wheeler, R. T., Kupiec, M., Magnelli, P., Abeijon, C. & Fink, G. R. (2003). A Saccharomyces cerevisiae mutant with increased virulence. Proc Natl Acad Sci U S A 100, 2766-2770.