Screening of boldenone and methylboldenone in bovine urine using disposable electrochemical immunosensors

Steroids

Volumn 71, Issue 9, 2006, Pages 760-767

  Lu, Huihui ^a   Conneely, Gráinne ^a   Pravda, Miloslav ^a   Guilbault, George G ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

Boldenone; ELISA; Immunosensor; Methylboldenone

Indexed keywords

ANTIBODY CONJUGATE; BOLDENONE; BOVINE SERUM ALBUMIN; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN G ANTIBODY; METHYLBOLDENONE; STEROID HORMONE; TETRAMETHYLBENZIDINE; UNCLASSIFIED DRUG;

AMPEROMETRY; ARTICLE; CATALYSIS; CONJUGATION; CONTROLLED STUDY; COW; CROSS REACTION; DILUTION; ELECTROCHEMICAL DETECTION; ELECTRODE; ENZYME LINKED IMMUNOSORBENT ASSAY; GAS CHROMATOGRAPHY; HORMONE DETERMINATION; HORMONE URINE LEVEL; HYDROLYSIS; IMMUNOASSAY; IMMUNOSENSOR; MASS SPECTROMETRY; METABOLITE; NONHUMAN; STATISTICAL ANALYSIS; URINALYSIS; URINE;

ANIMALS; BIOSENSING TECHNIQUES; CALIBRATION; CATTLE; DRUG RESIDUES; ELECTROCHEMISTRY; ENZYME-LINKED IMMUNOSORBENT ASSAY; ENZYMES, IMMOBILIZED; FEMALE; TESTOSTERONE;

ARMORACIA RUSTICANA; BOS TAURUS;

EID: 33745964005     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.steroids.2006.05.001     Document Type: Article

References (24)

1. van Puymbroeck M., Leyssens L., Vanderzande D., Gelan J., and Raus J. Metabolites in feces can be important markers for the abuse of anabolic steroids in cattle. Analyst 123 (1998) 2449-2452
2. van Puymbroeck M., Kuilman M.E.M., Maas R.F.M., Witlamp R.F., Leyssens L., Vanderzande D., et al. Identification of some important metabolites of boldenone in urine and feces of cattle by gas chromatography-mass spectrometry. Analyst 123 (1998) 2681-2686
3. Draisci R., Palleschi L., Ferretti E., Lucentini L., and Cammarata P. Quantitation of anabolic hormones and their metabolites in bovine serum and urine by liquid chromatography-tandem mass spectrometry. J Chromatogr A 870 (2000) 511-522
4. van Poucke C., and van Peteghem C. Development and validation of a multi-analyte method for the detection of anabolic steroids in bovine urine with liquid chromatography-tandem mass spectrometry. J Chromatogr B 772 (2002) 211-217
5. Draisci R., Palleschi L., Ferretti E., Lucentini L., and Quadri F.D. Confirmation analysis of 17β-boldenone, 17α-boldenone and androsta-1,4-diene-3,17-dione in bovine urine by liquid chromatography-tandem mass spectrometry. J Chromatogr B 789 (2003) 219-226
6. Nielen M.F., Rutgers P., van Bennekom E.O., Lasaroms J.J.P., and van Rhijn J.A. Confirmation analysis of 17β-boldenone, 17α-boldenone and androsta-1,4-diene-3,17-dione in bovine urine, faeces, feed and skin swab samples by liquid chromatography-electrospray ionization tandem mass spectrometry. J Chromatogr B 801 (2004) 273-283
7. Sangiorgi E., Polignano V., and Gardini S. Boldenone and related hormones quantification by liquid chromatography-mass spectrometry in urine and faeces after calf administration of boldenone undecanoate. Anal Chim Acta 529 (2005) 239-248
8. Popii V., and Baumann G. Laboratory measurement of growth hormone. Clin Chim Acta 350 (2004) 1-16
9. Wilke T.Y., and Utley D.J. Total testosterone, free-androgen index, calculated free testosterone, and free testosterone by analog RIA compared in hirsute women and in otherwise-normal women with altered binding of sex-hormone-binding globulin. Clin Chem 33 (1987) 1372-1375
10. Slaats E.H., Kennedy J.C., and Kruijswijk H. Interference of sex-hormone binding globulin in the "Coat-A-Count" testosterone no-extraction radioimmunoassay. Clin Chem 33 (1987) 300-302
11. Hungerford N.L., Sortais B., Smart C.G., McKinney A.R., Ridley D.D., Stenhouse A.M., et al. Analysis of anabolic steroids in the horse: development of a generic ELISA for the screening of 17α-alkyl anabolic steroid metabolites. J Steroid Biochem Mol Biol 96 (2005) 317-334
12. Hagedorn H.-W., Schulz R., and Jaeschke G. Identification and verification of the anabolic steroids boldenone in equine blood and urine by HPLC/ELISA. Biomed Chromatogr 8 (1994) 63-68
13. Hock B. Antibodies for immunosensors. Anal Chim Acta 347 (1997) 177-186
14. D'Orazio P. Biosensors in clinical chemistry. Chin Chim Acta 334 (2003) 41-69
15. Pemberton R.M., Hart J.P., and Foulkes J.A. Development of a sensitive, selective electrochemical immunoassay for progesterone in cow's milk, based on a disposable screen-printed amperometric biosensor. Electrichim Acta 43 (1998) 3567-3574
16. Kreuzer M.P., McCarthy R., Pravda M., and Guilbault G.G. Development of a electrochemical immunosensor for progesterone analysis in milk. Anal Lett 37 (2004) 943-956
17. Pemberton R.M., Mottram T.T., and Hart J.P. Development of a screen-printed carbon electrochemical immunosensor for picomolar concentrations of estradiol in human serum extracts. J Biochem Biophys Methods 63 (2005) 201-212
18. Conneely G. Development of single and multi-analyte affinity sensors for rapid detection of androgen residues in bovine urine, thesis, University College Cork, Cork, Ireland, 2005.
19. Jamal M., Crowe M.A., and Magner E. Characterization of the composition of bovine urine and its effect on the electrochemical analysis of the model mediator, p-aminophenol. Anal Chim Acta 554 (2005) 79-85
20. Andrew R. Clinical measurement of steroid metabolism. Best Pract Res Clin Endrocrinol Metab 15 (2001) 1-16
21. Lu H.-H., Conneely G., Crowe M.A., Aherne M., Pravda M., and Guilbault G.G. Screening for testosterone, methyltestosterone, 19-nortestosterone residues and their metabolites in bovine urine with enzyme-linked immunosorbent assay (ELISA). Anal Chim Acta 570 (2006) 116-123
22. Schänzer W., Horning S., and Donike M. Metabolism of anabolic steroids in humans: synthesis of 6β-hydroxy metabolites of 4-chloro-1,2-dehydro-17α-methyltestosterone, fluoxymesterone, and metandienone. Steroids 60 (1995) 353-366
23. Schänzer W., Geyer H., and Donike M. Metabolism of metandienone in man: identification and synthesis of conjugated excreted urinary metabolites, determination of excretion rates and gas chromatographic-mass spectrometric identification of bis-hydroxylated metabolites. J Steroid Biochem Mol Biol 38 (1991) 441-464
24. Blokland M.H., van Rossum H.J., Herbold H.A., Sterk S.S., Stephany R.W., and van Ginkel L.A. Metabolism of methyltestosterone, norethandrolone and methylboldenone in a heifer. Anal Chim Acta 529 (2005) 317-323