Reverse transcription-polymerase chain reaction and western blotting analysis for detection of p63 isoforms in uterine cervical cancers

International Journal of Gynecological Cancer

Volumn 16, Issue 4, 2006, Pages 1643-1647

  Lin, Z ^a   Nan, Y ^a   Zhang, X ^b   Zhao, Y ^a   Kim, C ^b   Kim, I ^b  

^a YANBIAN UNIVERSITY   (China)

^b Korea University College of Medicine   (South Korea)

Author keywords

p63 isoform; RT PCR; Uterine cervical cancer; Western blotting

Indexed keywords

ISOPROTEIN; MESSENGER RNA; PROTEIN P63;

ALPHA CHAIN; ARTICLE; BETA CHAIN; CANCER CELL CULTURE; CANCER DIAGNOSIS; CANCER TISSUE; CONTROLLED STUDY; ENDOMETRIUM CANCER; HUMAN; HUMAN CELL; HUMAN TISSUE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SQUAMOUS CELL CARCINOMA; UTERINE CERVIX CANCER; WART VIRUS; WESTERN BLOTTING;

BLOTTING, WESTERN; CARCINOMA, SQUAMOUS CELL; ENDOMETRIAL NEOPLASMS; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, DOMINANT; HELA CELLS; HUMANS; MEMBRANE PROTEINS; PAPILLOMAVIRIDAE; PROTEIN ISOFORMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR MARKERS, BIOLOGICAL; UTERINE CERVICAL NEOPLASMS;

EID: 33745957644     PISSN: 1048891X     EISSN: 15251438     Source Type: Journal    
DOI: 10.1111/j.1525-1438.2006.00638.x     Document Type: Article

References (13)

1. Yang A, Kaghad M, Wang Y et al. p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Mol Cell 1998;2:305-16.
2. Benard J, Douc-Rasy S., Ahomadegbe JC. TP53 family members and human cancers. Hum Mutat 2003;21:182-91.
3. Irwin MS, Kaelin WG. Role of the newer p53 family proteins in malignancy. Apoptosis 2001;6:17-29.
4. Reis-Filho JS, Schmitt FC. Taking advantage of basic research: p63 is a reliable myoepithelial and stem cell marker. Adv Anat Pathol 2002;9:280-9.
5. Signoretti S, Waltregny D, Dilks J et al. p63 is a prostate basal cell marker and is required for prostate development. Am J Pathol 2000;157:1764-75.
6. Chen YK, Hsue SS, Lin LM. Expression of p63 (TA and ΔN isoforms) in human primary well differentiated buccal carcinomas. Int J Oral Maxillolfac Surg 2004;33:493-7.
7. Nylander K, Vojtesek B, Nenutil R et al. Differential expression of p63 isoforms in normal tissues and neoplastic cells. J Pathol 2002;198:417-27.
8. Di Como CJ, Urist MJ, Babayan I et al. p63 expression profiles in human normal and tumor tissues. Clin Cancer Res 2002;8:494-501.
9. Geddert H, Kiel S, Heep HJ, Gabbert HE, Sarbia M. The role of p63 and ΔNp63 (p40) protein expression and gene amplification in esophageal carcinogenesis. Hum Pathol 2003;34:850-6.
10. Urist MJ, Di Como CJ, Lu ML et al. Loss of p63 expression is associated with tumor progression in bladder cancer. Am J Pathol 2002;161:1199-206.
11. Chilosi M, Zamo A, Brighenti A et al. Constitutive expression of deltaN-p63alpha isoform in human thymus and thymic epithelial tumours. Virchows Arch 2003;443:175-83.
12. Massion PP, Taflan PM, Rahman SMJ et al. Significance of p63 amplification and overexpression in lung cancer development and prognosis. Cancer Res 2003;63:7113-21.
13. Wang TY, Chen BF, Yang YC et al. Histologic and immunophenotypic classification of cervical carcinomas by expression of the p53 homologue p63: a study of 250 cases. Hum Pathol 2001;32:479-86.