Design, development and evaluation of a competitive RT-PCR for quantitation of GBV-C RNA

Journal of Virological Methods

Volumn 136, Issue 1-2, 2006, Pages 58-64

  Ruiz, Vanesa ^a   Espínola, Lidia ^a   Mathet, Verónica Lidia ^a   Perandones, Carlos Edgardo ^a   Oubiña, José Raúl ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

Competitive RT PCR; GB virus C; RNA quantitation; Viral load

Indexed keywords

VIRUS RNA;

ANALYTIC METHOD; ARTICLE; BLOOD DONOR; CONTROLLED STUDY; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DILUTION; HEMATOLOGIC DISEASE; HEPATITIS G; HEPATITIS G VIRUS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTERMETHOD COMPARISON; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SERUM; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS GENOME; VIRUS LOAD; VIRUS VIRULENCE;

GB VIRUS C; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

GB VIRUS C; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 33745877608     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.03.032     Document Type: Article

References (37)

1. Bogard M., Buffet-Janvresse C., Cantaloube J.F., Biagini P., Duverlie G., Castelain S., Izopet J., Dubois M., Defer C., Lepot I., Coste J., Marcellin P., Martinot-Peignoux M., Halfon P., Gerolami V., Frangeul L., Pawlotsky J.M., Roudot-Thoraval F., Dussaix E., Loiseau P., Ravera N., Lewin P., Lamoril J., Lerable J., Bouchardeau F., Laperche S., Mariotti M., Lafrère J.J., Nicot T., Denis F., Gassain M., Mérel P., Trépo C., Thiers V., Bréchot C., Férec C., Mercier B., Stoll-Keller F., Palmer P., and Lebon P. GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum. J. Clin. Microbiol. 35 (1997) 3298-3300
2. Cantaloube J.F., Charrel R.N., Attoui H., Biagini P., De Micco P., and De Lamballarie X. Evaluation of four PCR systems amplifying different genomic regions for molecular diagnosis of GB virus C infections. J. Virol. Methods 64 (1997) 131-135
3. Fan X., Xu Y., Solomon H., Ramrakhiani S., Neuschwander-Tetri B.A., and Di Bisceglie A.M. Is hepatitis G/GB virus-C virus hepatotropic? Detection of hepatitis G/GB virus-C viral RNA in liver and serum. J. Med. Virol. 58 (1999) 160-164
4. Fogeda M., Navas S., Martin J., Casqueiro M., Rodriguez E., Arocena C., and Carreño V. In vitro infection of human peripheral blood mononuclear cells by GB virus C/hepatitis G virus. J. Virol. 73 (1999) 4052-4061
5. Fogeda M., Lopez-Alcorocho J.M., Bartolome J., Arocena C., Martin M.A., and Carreño V. Existence of distinct GB virus C/hepatitis G virus variants with different tropism. J. Virol. 74 (2000) 7936-7942
6. George S.L., and Varmaz D. What you need to know about GB virus C. Curr. Gastroenterol. Rep. 7 (2005) 54-62
7. Halasz R., Sällberg M., Lundholm S., Andersson G., Lager B., Glaumann H., and Weiland O. The GB virus C/hepatitis G virus replicates in hepatocytes without causing liver disease in healthy blood donors. J. Infect. Dis. 182 (2000) 1756-1760
8. Hawkins A., Davison F., and Simmonds P. Comparison of plasma virus loads among individuals infected with hepatitis C virus (HCV) genotypes 1, and 3 by Quantiplex HCV RNA assay versions 1 and 2, Roche Monitor assay, and an in-house limiting dilution method. J. Clin. Microbiol. 35 (1997) 187-192
9. Jung S., Knauer O., Donhauser N., Eichenmüller M., Helm M., Fleckenstein B., and Reil H. Inhibition of HIV strains by GB virus C in cell culture can be mediated by CD4 and CD8 T-lymphocyte derived soluble factors. AIDS 19 (2005) 1267-1272
10. Lanford R.E., Sureau C., Jacob J.R., White R., and Fuerst T.R. Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR. Virology 202 (1994) 606-614
11. Lanford R.E., Chavez D., Chisari F.V., and Sureau C. Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR. J. Virol. 69 (1995) 8079-8083
12. Laskus T., Radkowski M., Wang L.F., Vargas H., and Rakela J. Lack of evidence for hepatitis G virus replication in the livers of patients coinfected with hepatitis C and G viruses. J. Virol. 71 (1997) 7804-7806
13. Laskus T., Radkowski M., Wang L.F., Vargas H., and Rakela J. Detection of hepatitis G virus replication sites by using highly strand-specific Tth-based reverse transcriptase PCR. J. Virol. 72 (1998) 3072-3075
14. Laskus T., Radkowski M., Jablonska J., Kibler K., Wilkinson J., Adair D., and Rakela J. Human immunodeficiency virus facilitates infection/replication of hepatitis C virus in native human macrophages. Blood 103 (2004) 3854-3859
15. Leary T.P., Muerhoff A.S., Simons J.N., Pilot-Matias T.J., Erker J.C., Chalmers M.L., Schlauder G.G., Dawson G.J., Desai S.M., and Mushahwar I.K. Sequence and genomic organization of GBV-C: a novel member of the Flaviviridae associated with human non-A-E hepatitis. J. Med. Virol. 48 (1996) 60-67
16. Lefrere J.J., Lerable J., Mariotti M., Bogard M., Thibault V., Frangeul L., Loiseau P., Bouchardeau F., Laperche S., Pawlotsky J.M., Cantaloube J.F., Biagini P., de Lamballerie X., Izopet J., Defer C., Lepot I., Poveda J.D., Dussaix E., Gerolami V., Halfon P., Buffet-Janvresse C., Ferec C., Mercier B., Marcellin P., Martinot-Peignoux M., and Gassain M. Lessons from a multicentre study of the detectability of viral genomes based on a two-round quality control of GB virus C (GBV-C)/hepatitis G virus (HGV) polymerase chain reaction assay. J. Virol. Methods 85 (2000) 117-124
17. Liu F., Knight G.B., Abel G., and Agnello V. A competitive reverse transcription-polymerase chain reaction assay for quantitation of GB virus C/hepatitis G virus RNA that circumvents heteroduplex artefact. J. Virol. Methods 79 (1999) 149-159
18. Madejon A., Fogeda M., Bartolome J., Pardo M., Gonzalez C., Cotonat T., and Carreño V. GB virus C RNA in serum, liver and peripheral blood mononuclear cells from patients with chronic hepatitis B, C, and D. Gastroenterology 113 (1997) 573-578
19. Mellor J., Hawkins A., and Simmonds P. Genotype dependence of hepatitis C virus load measurement in commercially available quantitative assays. J. Clin. Microbiol. 37 (1999) 2525-2532
20. Nattermann J., Nischalke H.D., Kupfer B., Rockstroh J., Hess L., Sauerbruch T., and Spengler U. Regulation of CC chemokine receptor 5 in hepatitis G virus infection. AIDS 17 (2003) 1457-1462
21. Negro F., Giostra E., Krawczynski K., Quadri R., Rubbia-Brandt L., Mentha G., Colucci G., Perrin L., and Hadengue A. Detection of intrahepatic hepatitis C virus replication by strand-specific semiquantitative RT-PCR: preliminary application to the liver transplantation model. J. Hepatol. 29 (1998) 1-11
22. Negro F., Krawczynski K., Quadri R., Rubbia-Brandt L., Mondelli M., Zarski J.P., and Hadengue A. Detection of genomic- and minus-strand of hepatitis C virus RNA in the liver of chronic hepatitis C patients by strand-specific semiquantitative reverse-transcriptase polymerase chain reaction. Hepatology 29 (1999) 536-542
23. Oubiña J.R., Quarleri J.F., Rudzinski M., Parks C., Badía I., and González Cappa S.M. Genomic characterization of hepatitis C virus isolates from Argentina. J. Med. Virol. 47 (1995) 97-104
24. Pessoa M.G., Terrault N.A., Detmer J., Kolberg J., Collins M., Hassoba H.M., and Wright T.L. Quantitation of hepatitis G and C viruses in the liver: evidence that hepatitis G virus is not hepatotropic. Hepatology 27 (1998) 877-880
25. Quarleri J.F., Mathet V.L., Feld M., Ferrario D., della Latta M.P., Verdun R., Sanchez D.O., and Oubiña J.R. GB virus C/hepatitis G virus groups and subgroups: classification by a restriction fragment length polymorphism method based on phylogenetic analysis of the 5′ untranslated region. J. Clin. Microbiol. 37 (1999) 1340-1347
26. Quarleri J.F., Bussy M.V., Mathet V.L., Ruiz V., Iácono R., Lu L., Robertson B.H., and Oubiña J.R. In vitro detection of dissimilar amounts of hepatitis C virus (HCV) subtype-specific RNA genomes in mixes prepared from sera of persons infected with a single HCV genotype. J. Clin. Microbiol. 41 (2003) 2727-2733
27. Radkowski M., Wang L.F., Cianciara J., Rakela J., and Laskus T. Analysis of hepatitis G virus/GB virus C quasispecies and replication sites in human subjects. Biochem. Biophys. Res. Commun. 258 (1999) 296-299
28. Radkowski M., Kubicka J., Kisiel E., Cianciara J., Nowicki M., Rakela J., and Laskus T. Detection of active hepatitis C virus and hepatitis G virus/GB virus C replication in bone marrow in human subjects. Blood 95 (2000) 3986-3989
29. Saito S., Tanaka K., Kondo M., Morita K., Kitamura T., Kiba T., Numata K., and Sekihara H. Plus- and minus-stranded hepatitis G virus RNA in liver tissue and in peripheral blood mononuclear cells. Biochem. Biophys. Res. Commun. 237 (1997) 288-291
30. Schleicher S.B., and Flehmig B. Genotyping of GB virus C by restriction pattern analysis of the 5′ untranslated region. J. Med. Virol. 71 (2003) 226-232
31. Seipp S., Scheidel M., Hofmann W.J., Tox U., Theilmann L., Goeser T., and Kallinowski B. Hepatotropism of GB virus C (GBV-C): GBV-C replication in human hepatocytes and cells of human hepatoma cell lines. J. Hepatol. 30 (1999) 570-579
32. Shimizu Y.K., Hijikata M., Kiyohara T., Kitamura Y., and Yoshikura H. Replication of GB virus C (hepatitis G virus) in interferon-resistant Daudi cells. J. Virol. 73 (1999) 8411-8414
33. Stapleton J.T. GB virus type C/hepatitis G virus. Semin. Liver Dis. 23 (2003) 137-148
34. Stapleton J.T., Williams C.F., and Xiang J. GB virus type C: a beneficial infection?. J. Clin. Microbiol. 42 (2004) 3915-3919
35. +-purified RNA. Hepatology 32 (2000) 382-387
36. Xiang J., Wunschmann S., Schmidt W., Shao J., and Stapleton J.T. Full-length GB virus C (hepatitis G virus) RNA transcripts are infectious in primary CD4-positive T cells. J. Virol. 74 (2000) 9125-9133
37. Xiang J., George S.L., Wunschmann S., Chang Q., Klinzman D., and Stapleton J.T. Inhibition of HIV-1 replication by GB virus C infection through increases in RANTES, MIP-1alpha, MIP-1beta, and SDF-1. Lancet 363 (2004) 2040-2046