Rapid detection of fungal endophytes in grasses for large-scale studies

Functional Ecology

Volumn 20, Issue 4, 2006, Pages 736-742

  Koh, S ^a   Vicari, M ^b   Ball, J P ^c   Rakocevic, T ^b   Zaheer, S ^b   Hik, D S ^a   Bazely, D R ^b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b YORK UNIVERSITY   (Canada)

^c SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Endophytic fungi; Festuca spp.; Forensic ecology; Immunoblot card; Monoclonal antibodies; Neotyphodium; Pooideae

Indexed keywords

DETECTION METHOD; ENDOPHYTE; FUNGUS; GRASS;

FESTUCA ARUNDINACEA; LOLIUM PERENNE; NEOTYPHODIUM; PHIPPSIA NEOARCTICA; POACEAE;

EID: 33745865181     PISSN: 02698463     EISSN: 13652435     Source Type: Journal    
DOI: 10.1111/j.1365-2435.2006.01150.x     Document Type: Article

References (27)

1. Allen, V.G. & Segarra, E. (2001) Anti-quality components in forage: overview, significance, and economic impact. Journal of Range Management 54, 409-412.
2. Bacon, C.W. (1995) Toxic endophyte-infected tall fescue and range grasses: historic perspectives. Journal of Science 73, 861-870.
3. Bazely, D.R., Vicari, M., Emmerich, S., Filip, L., Lin, D. & Inman, A. (1997) Interactions between herbivores and endophyte-infected Festuca rubra from the Scottish Islands of St. Kilda, Benbecula and Rum. Journal of Applied Ecology 34, 847-860.
4. Brem, D. & Leuchtmann, A. (2001) Epichloë grass endophytes increase herbivore resistance in the woodland grass Brachypodium sylvaticum. Oecologia (Heidelb) 126, 522-530.
5. Brem, D. & Leuchtmann, A. (2002) Intraspecific competition of endophyte infected vs uninfected plants of two woodland grass species. Oikos 96, 281-290.
6. Cheplick, G.P. (1998) Genotypic variation in the regrowth of Lolium perenne following clipping: effects of nutrients and endophytic fungi. Functional Ecology 12, 176-184.
7. Clay, K. (1989) Clavicipitaceous fungal endophytes of grasses: their potential as biocontrol agents. Mycological Research 92, 1-12.
8. Faeth, S.H. & Sullivan, T.J. (2003) Mutualistic asexual endophytes in a native grass are usually parasitic. American Naturalist 161, 310-325.
9. Faeth, S.H., Helander, M.L. & Saikkonen, K.T. (2004) Asexual Neotyphodium endophytes in a native grass reduce competitive abilities. Ecology Letters 7, 304-313.
10. Gams, W., Petrini, O. & Schmidt, D. (1990) Acremonium uncinatum, a new endophyte in Festuca pratensis. Mycotaxon 37, 67-71.
11. Gwinn, K.D., Collins-Shepard, M.H. & Reddick, B.B. (1991) Tissue print-immunoblot, an accurate method for the detection of Acremonium coenophialium in tall fescue. Phytopathology 81, 747-748.
12. Hahn, H., Huth, W., Schöberlein, W. & Diepenbrock, W. (2003) Detection of endophytic fungi in Festuca spp. by means of tissue print immunoassay. Plant Breeding 122, 217-222.
13. Hiatt, E.E., Hill, N.S., Bouton, J.H. & Mims, C.W. (1997) Monoclonal antibodies for detection of Neotyphodium coenophialum. Crop Science 37, 1265-1269.
14. Hiatt, E.E., Hill, N.S., Bouton, J.H. & Stuedemann, J.A. (1999) Tall fescue endophyte detection: commercial immunoblot test kit compared with microscopic analysis. Crop Science 39, 796-799.
15. Kerfoot, W.C., Robbins, JA. & Weider, L.J. (1999) A new approach to historical reconstruction: combining descriptive and experimental paleolimnology. Limnology and Oceanography 44, 1232-1247.
16. Leyronas, C. & Raynal, G. (2001) Presence of Neotyphodium-like endophytes in European grasses. Annals of Applied Biology 139, 119-127.
17. Mack, R.N. (1981) Invasion of Bromus tectorum L. into Western North American ecological chronicle. AgroEcosystems 7, 145-165.
18. Menna, M.E. & Waller, J.E. (1986) Visual assessment of seasonal changes in amount of mycelium of Acremonium loliae in leaf sheaths of perennial ryegrass. New Zealand Journal of Agricultural Research 29, 111-116.
19. Nan, Z.B. (2000) Neotyphodium in native grasses in China and observations on endophyte/host interactions. 4th International Neotyphodium-Grass Interactions Symposium, Book of Abstracts, p. 10. University of Paderborn, Soest, Germany.
20. Rudgers, J.A., Koslow, J.M. & Clay, K. (2004) Endophytic fungi alter relationships between diversity and ecosystem properties. Ecology Letters 7, 42-51.
21. Saikkonen, K., Ahlholm, J., Helander, M., Lehtimäki, S. & Niemeläinen, O. (2000) Endophytic fungi in wild and cultivated grasses in Finland. Ecography 23, 360-366.
22. Sampson, K. (1933) The systemic infection of grasses by Epichloë typhina (Pers.) Tul. Transactions of the British Mycological Society 18, 30-47.
23. Shipton, W.A. & Brown, J.F. (1962) A whole-leaf clearing and staining technique to demonstrate host-pathogen relationships of wheat stem rust. Phytopathology 52, 1313.
24. Siegel, S. & Castellan, N.J. Jr (1988) Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill, London.
25. Siegel, M.R., Schardl, C.L. & Phillips, T.D. (1995) Incidence and compatibility of nonclavicipitaceous fungal endophytes in Festuca and Lolium species. Mycologia. 87, 196-202.
26. Wheatley, W.M. & Simpson, W.R. (2000) Tissue immunoblot procedure - stability of Neotyphodium endophyte antigen on nitrocellulose membrane. Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium (eds V.H. Paul & P.D. Dapprich), pp. 145-147. Universität- Gesamthochschule Paderborn, Abteilung Soest, Germany.
27. White, J.F. Jr (1993) Endophyte-host associations in grasses. 19. A systematic study of some sympatric species of Epichloë. England. Mycologia 85, 444-455.