Tall fescue endophyte detection: Commercial immunoblot test kit compared with microscopic analysis

Crop Science

Volumn 39, Issue 3, 1999, Pages 796-799

  Hiatt III, E E ^a   Hill, N S ^a   Bouton, J H ^a   Stuedemann, J A ^b  

^a UNIVERSITY OF GEORGIA   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DISEASE DETECTION; IMMUNOANALYSIS; MICROSCOPY; PLANT BREEDING; WESTERN BLOTTING;

FESTUCA ARUNDINACEA; NEOTYPHODIUM COENOPHIALUM;

EID: 0032969910     PISSN: 0011183X     EISSN: None     Source Type: Journal    
DOI: 10.2135/cropsci1999.0011183X003900030030x     Document Type: Article

References (12)

1. Adcock, R.A., N.S. Hill, J.H. Bouton, H.R. Boerma, and G.O. Ware. 1997. Symbiont regulation and reducing ergot alkaloid concentration by breeding endophyte-infected tall fescue. J. Chem. Ecology 23:691-704.
2. Clark, E.M., J.F. White, and R.M. Patterson. 1983. Improved histochemical techniques for the detection of Acremonium coenophialum in tall fescue and methods of in vitro culture of the fungus. J. Microbiol. Meth. 1:149-155.
3. Gwinn, K.D., M.H. Collins-Shepard, and B.B. Reddick. 1991. Tissue print-immunoblot, an accurate method for the detection of Acremonium coenophialum in tall fescue. Phytopathology 81:747-748.
4. Hiatt, III, E.E. and N.S. Hill. 1997. Neotyphodium coenophialum mycelial protein and herbage mass effects on ergot alkaloid concentration in tall fescue. J. Chem. Ecology 23:2721-2736.
5. Hiatt, III, E.E., N.S. Hill, J.H. Bouton, and C.W. Mims. 1997. Monoclonal antibodies for detection of Neotyphodium coenophialum. Crop Sci. 37:1265-1269.
6. Johnson, M.C., T.C. Pirone, M.R. Seigel, and D.R. Varney. 1982. Detection of Epichloe typhina in tall fescue by means of enzyme-linked immunosorbent assay. Phytopathology 72:647-650.
7. Little, T.M., and F.J. Hills. 1978. Agricultural experimentation design and analysis. John Wiley and Sons, New York.
8. Musgrave, D.R., T.A. Grose, G.C.M. Latch, and M.J. Christensen. 1986. Purification and characterisation of the antigens of endophytic fungi isolated from Lolium perenne and Festuca arundinacea in New Zealand. N.Z. J. Agric. Res. 29:121-128.
9. Reddick, B.B. 1988. Detection of the tall fescue endophyte with emphasis on enzyme-linked immunosorbent assay. J. Prod. Agric. 1:133-136.
10. Reddick, B.B., and M.H. Collins. 1988. An improved method for detection of Acremonium coenophialum in tall fescue plants. Phytopathology 78:418-420.
11. Roitt, I.M. 1994. Essential immunology. 8th ed. Blackwell Scientific Publications, Cambridge, MA.
12. Welty, R.E., M.D. Azevedo, and T.M. Cooper. 1987. Influence of moisture-content, temperature, and length of storage on seed-germination and survival of endophytic fungi in seeds of tall fescue and perennial ryegrass. Phytopathology 77:893-900.