DNAse treatment following thawing of Cryopreserved PBMC is a procedure suitable for lymphocyte functional studies

Journal of Immunological Methods

Volumn 313, Issue 1-2, 2006, Pages 209-213

  García Piñeres, Alfonso J ^a   Hildesheim, Allan ^b   Williams, Marcus ^a   Trivett, Matthew ^a   Strobl, Susan ^a   Pinto, Ligia A ^a  

^a SAIC FREDERICK INC   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Cytokine; DNAse; Flow cytometry; PBMC; Proliferation

Indexed keywords

CYTOKINE; DEOXYRIBONUCLEASE; LYMPHOCYTE SURFACE MARKER;

ARTICLE; CELL ASSAY; CELL FUNCTION; CELL PROLIFERATION; CELL VIABILITY; CRYOPRESERVATION; HUMAN; HUMAN CELL; IMMUNE FUNCTION TEST; IMMUNOLOGICAL PARAMETERS; LYMPHOCYTE FUNCTION; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PHENOTYPE; PRIORITY JOURNAL; PROTEIN INDUCTION; THAWING;

ANTIGENS; ANTIGENS, SURFACE; B-LYMPHOCYTES; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL PROLIFERATION; CELL SURVIVAL; CRYOPRESERVATION; CYTOKINES; DEOXYRIBONUCLEASES; HUMANS; INTERFERON TYPE II; INTERLEUKINS; KILLER CELLS, NATURAL; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; MONOCYTES; TETRADECANOYLPHORBOL ACETATE;

EID: 33745855520     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.04.004     Document Type: Article

References (6)

1. Beck C., Nguyen X.D., Kluter H., and Eichler H. Effect of recombinant human deoxyribonuclease on the expression of cell adhesion molecules of thawed and processed cord blood hematopoietic progenitors. Eur. J. Haematol. 70 (2003) 136
2. Brinkmann V., Reichard U., Goosmann C., Fauler B., Uhlemann Y., Weiss D.S., Weinrauch Y., and Zychlinsky A. Neutrophil extracellular traps kill bacteria. Science 303 (2004) 1532
3. Disis M.L., Dela Rosa C., Goodell V., Kuan L.Y., Chang J.C., Kuus-Reichel K., Clay T.M., Kim Lyerly H., Bhatia S., Ghanekar S.A., Maino V.C., and Maecker H.T. Maximizing the retention of antigen specific lymphocyte function after cryopreservation. J. Immunol. Methods (2005)
4. Eichler H., Beck C., Bernard F., Bugert P., and Kluter H. Use of recombinant human deoxyribonuclease (DNase) for processing of a thawed umbilical cord blood transplant in a patient with relapsed acute lymphoblastic leukemia. Ann. Hematol. 81 (2002) 170
5. Pinto L.A., Trivett M.T., Wallace D., Higgins J., Baseler M., Terabe M., Belyakov I.M., Berzofsky J.A., and Hildesheim A. Fixation and cryopreservation of whole blood and isolated mononuclear cells: influence of different procedures on lymphocyte subset analysis by flow cytometry. Cytom. B Clin. Cytom. 63 (2005) 47
6. Smith J.G., Liu X., Kaufhold R.M., Clair J., and Caulfield M.J. Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus. Clin. Diagn. Lab. Immunol. 8 (2001) 871