Expression of actin mutants to study their roles in cardiomyopathy

Canadian Journal of Physiology and Pharmacology

Volumn 84, Issue 1, 2006, Pages 111-119

  Rutkevich, Lori A ^a   Teal, David J ^a   Dawson, John F ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Actin; Baculovirus expression; Cardiac muscle proteins; Polymerization kinetics; Protein expression

Indexed keywords

ACTIN;

ACTIN POLYMERIZATION; CARDIOMYOPATHY; CONFERENCE PAPER; CYTOLOGY; GENE MUTATION; HUMAN; HUMAN CELL; INSECT CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION;

ACTINS; ANIMALS; BACULOVIRIDAE; CARDIOMYOPATHIES; CELL LINE; HUMANS; INSECTS; MUTANT PROTEINS; PLASMIDS; PROTEIN FOLDING; YEASTS;

EID: 33745757777     PISSN: 00084212     EISSN: None     Source Type: Journal    
DOI: 10.1139/y05-140     Document Type: Conference Paper

References (26)

1. Anthony Akkari, P., Nowak, K.J., Beckman, K., Walker, K.R., Schachat, F., and Laing, N.G. 2003. Production of human skeletal alpha-actin proteins by the baculovirus expression system. Biochem. Biophys. Res. Commun. 307: 74-79. doi: 10.1016/S0006-291X(03)01133-1. PMID: 12849983.
2. Ayscough, K.R., and Drubin, D.G. 1996. Actin: general principles from studies in yeast. Annu. Rev. Cell Dev. Biol. 12: 129-160. doi: 10.1146/annurev.cellbio.12.1.129. PMID: 8970724.
3. Buzan, J., Du, J., Karpova, T., and Frieden, C. 1999. Histidine-tagged wild-type yeast actin: its properties and use in an approach for obtaining yeast actin mutants. Proc. Natl. Acad. Sci. USA, 96: 2823-2827. doi: 10.1073/pnas.96.6.2823. PMID: 10077595.
4. Chen, X., Cook, R.K., and Rubenstein, P.A. 1993. Yeast actin with a mutation in the "hydrophobic plug" between subdomains 3 and 4 (1266d) displays a cold-sensitive polymerization defect. J. Cell Biol. 123: 1185-1195. doi: 10.1083/jcb.123.5.1185. PMID: 8245125.
5. Chowdhury, S., Smith, K.W., and Gustin, M.C. 1992. Osmotic stress and the yeast cytoskeleton: phenotype-specific suppression of an actin mutation. J. Cell Biol. 118: 561-571. doi: 10.1083/jcb.118.3.561. PMID: 1639843.
6. Cook, R.K., Blake, W.T., and Rubenstein, P.A. 1992. Removal of the amino-terminal acidic residues of yeast actin. Studies in vitro and in vivo. J. Biol. Chem. 267: 9430-9436. PMID: 1349604.
7. Feng, L., Kim, E., Lee, W.L., Miller, C.J., Kuang, B., Reisler, E., and Rubenstein, P.A. 1997. Fluorescence probing of yeast actin subdomain 3/4 hydrophobic loop 262-274. Actin-actin and actin-myosin interactions in actin filaments. J. Biol. Chem. 272: 16829-16837. doi: 10.1074/jbc.272.27.16829. PMID: 9201989.
8. Hennessey, E.S., Harrison, A., Drummond, D.R., and Sparrow, J.C. 1992. Mutant actin: a dead end? J. Muscle Res. Cell Motil. 13: 127-131. doi: 10.1007/BF01874149. PMID: 1597510.
9. Holmes, K.C., Popp, D., Gebhard, W., and Kabsch, W. 1990. Atomic model of the actin filament. Nature, 347: 44-49. doi: 10.1038/347044a0. PMID: 2395461.
10. Joel, P.B., Fagnant, P.M., and Trybus, K.M. 2004. Expression of a nonpolymerizable actin mutant in sf9 cells. Biochemistry, 43: 11554-11559. doi: 10.1021/bi048899a. PMID: 15350141.
11. Kuang, B., and Rubenstein, P.A. 1997. Beryllium fluoride and phalloidin restore polymerizability of a mutant yeast actin (v266g,1267g) with severely decreased hydrophobicity in a subdomain 3/4 loop. J. Biol. Chem. 272: 1237-1247. PMID: 8995427.
12. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage t4. Nature, 227: 680-685. doi: 10.1038/227680a0. PMID: 5432063.
13. Lotka, A.J. 1924. Elements of physical biology. Williams & Wilkins. Baltimore, MD.
14. Marston, S.B., and Hodgkinson, J.L. 2001. Cardiac and skeletal myopathies: can genotype explain phenotype? J. Muscle Res. Cell Motil. 22: 1-4. doi: 10.1023/A:1010355716511. PMID: 11563546.
15. Mogensen, J., Klausen, I.C., Pedersen, A.K., Egeblad, H., Bross, P., Kruse, T.A., et al. 1999. Alpha-cardiac actin is a novel disease gene in familial hypertrophic cardiomyopathy. J. Clin. Invest. 103: R39-R43. PMID: 10330430.
16. Musib, R., Wang, G., Geng, L., and Rubenstein, P.A. 2002. Effect of polymerization on the subdomain 3/4 loop of yeast actin. J. Biol. Chem. 277: 22699-22709. PMID: 11959868.
17. Olson, T.M., Michels, V.V., Thibodeau, S.N., Tai, Y.S., and Keating, M.T. 1998. Actin mutations in dilated cardiomyopathy, a heritable form of heart failure. Science, 280: 750-752. doi: 10.1126/science.280.5364.750. PMID: 9563954.
18. Olson, T.M., Doan, T.P., Kishimoto, N.Y., Whitby, F.G., Ackerman, M.J., and Fananapazir, L. 2000. Inherited and de novo mutations in the cardiac actin gene cause hypertrophic cardiomyopathy. J. Mol. Cell. Cardiol. 32: 1687-1694. doi: 10.1006/jmcc.2000. 1204. PMID: 10966831.
19. O'Reilly, D.R. 1997. Use of baculovirus expression vectors. Methods Mol. Biol. 62: 235-246. PMID: 9108525.
20. Privalov, P.L., and Gill, S.J. 1988. Stability of protein structure and hydrophobic interaction. Adv. Protein Chem. 39: 191-234. PMID: 3072868.
21. Spiess, C., Meyer, A.S., Reissmann, S., and Frydman, J. 2004. Mechanism of the eukaryotic chaperonin: protein folding in the chamber of secrets. Trends Cell Biol. 14: 598-604. doi: 10.1016/j.tcb.2004.09.015. PMID: 15519848.
22. Spudich, J.A. 2001. The myosin swinging cross-bridge model. Nat. Rev. Mol. Cell Biol. 2: 387-392. doi: 10.1038/35073086. PMID: 11331913.
23. Steinmetz, M.O., Stoffler, D., Hoenger, A., Bremer, A., and Aebi, U. 1997. Actin: from cell biology to atomic detail. J. Struct. Biol. 119: 295-320. doi: 10.1006/jsbi.1997.3873. PMID: 9245769.
24. Thulasiraman, V., Ferreyra, R.G., and Frydman, J. 2000. Monitoring actin folding. Purification protocols for labeled proteins and binding to dnase i-sepharose beads. Methods Mol Biol. 140: 161-167. PMID: 11484485.
25. Vang, S., Corydon, T.J., Borglum, A.D., Scott, M.D., Frydman, J., Mogensen, J., Gregersen, N., and Bross, P. 2005. Actin mutations in hypertrophic and dilated cardiomyopathy cause inefficient protein folding and perturbed filament formation. FEBS J. 272: 2037-2049. doi: 10.1111/J.1742-4658.2005.04630. x. PMID: 15819894.
26. Wong, W.W., Doyle, T.C., Cheung, P., Olson, T.M., and Reisler, E. 2001. Functional studies of yeast actin mutants corresponding to human cardiomyopathy mutations. J. Muscle Res. Cell Motil. 22: 665-674. doi: 10.1023/A:1016354308436. PMID: 12222827.