Actin mutations in dilated cardiomyopathy, a heritable form of heart failure

Science

Volumn 280, Issue 5364, 1998, Pages 750-752

  Olson, Timothy M ^a   Michels, Virginia V ^b   Thibodeau, Stephen N ^b   Tai, Yin Shan ^a   Keating, Mark T ^c  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b MAYO CLINIC   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN;

ARTICLE; GENETIC LINKAGE; HEART DILATATION; HEART MUSCLE CELL; HUMAN; MISSENSE MUTATION; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DETERMINATION;

EID: 0032076955     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5364.750     Document Type: Article

References (39)

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19. Primers were designed according to the published genomic DNA sequence tor ACTC [H. Hamada, M. G. Petrino, T. Kakunaga, Proc. Natl. Acad. Sci. U.S.A. 79, 5901 (1982)] and OLIGO 4.03 Primer Analysis Software (National Biosciences, Plymouth, MN). The forward primer for exon 5 overlaps 5 base pairs (bp) of coding sequence to avoid a repetitive dinucleotide sequence near the intron-exon boundary. Primer sequences are as follows: exon1F, 5′-CCCCTGAAGCTGTGCCAAGA-3′; exon1R, 5′-GGCTCGGCGGGAAGTTTAC-3′; exon2F, 5′-TAAATGGACAAGACACTGATTAT-3′; exon2R, 5′-CAGCAAGGTCGGTGACTT-3′; exon3F, 5′-GCTAGAGCAGTGGTGTTGTC-3′; exon3R, 5′-AGGTAGGCGGATTCAGTG-3′; exon4F, 5′-CTCACTGAATCCGCCTACCT-3′; exon4R, 5′-CTACACCAGACCCTACAACTC-3′; exon5F, 5′-GACTCGTTCCCAGGTATG-3′; exon5R, 5′-GATCTCCCACTCACAAAAG-3′; exon 6F, 5′-AAGTTTTTGTTTTCTTCTGC-3′; exon 6R, 5′-CATAATACCGTCATCCTGA-3′. Polymerase chain reaction (PCR) product sizes for exons 1 to 6 are 167, 457, 292, 291, 222, and 214 bp, respectively.
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39. We thank D. Schaid, F. Miller, D. Driscoll, G. Orsmond, R. Shaddy, and H. Holtzer for support and helpful discussions; W. Edwards (print for Fig. 2); and J. Ross, D. Renlund, J. Mason, J. Anderson, M. Karst, and N. Kishimoto for assistance. Supported by Clinician Scientist Award 96004630 from the American Heart Association (T.M.O.), NIH SCOR grant 5-P50-HL-53773, Project 6 (M.T.K.), the John Patrick Albright Foundation, Public Health Services Research Grant M01-RR00064 from the National Center for Research Resources, and the Technology Access Section of the Utah Genome Center.